Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A0O31_RS03510 Genome accession   NZ_CP016312
Coordinates   690144..691229 (+) Length   361 a.a.
NCBI ID   WP_071676687.1    Uniprot ID   -
Organism   Thermus brockianus strain GE-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 685144..696229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A0O31_RS03485 (A0O31_00720) ribH 685287..685760 (+) 474 WP_071676682.1 6,7-dimethyl-8-ribityllumazine synthase -
  A0O31_RS03490 (A0O31_00721) - 685747..686151 (-) 405 WP_071676683.1 DUF4395 domain-containing protein -
  A0O31_RS03495 (A0O31_00722) pgeF 686223..686948 (+) 726 WP_071676684.1 peptidoglycan editing factor PgeF -
  A0O31_RS03500 (A0O31_00723) - 686984..687466 (+) 483 WP_071676685.1 YqeG family HAD IIIA-type phosphatase -
  A0O31_RS03505 (A0O31_00724) pilF 687463..690132 (+) 2670 WP_071676686.1 type IV pilus assembly ATPase PilB Machinery gene
  A0O31_RS03510 (A0O31_00725) pilT 690144..691229 (+) 1086 WP_071676687.1 type IV pilus twitching motility protein PilT Machinery gene
  A0O31_RS03515 (A0O31_00726) gatB 691247..692656 (+) 1410 WP_071676688.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  A0O31_RS03520 (A0O31_00727) purM 692662..693663 (+) 1002 WP_071676689.1 phosphoribosylformylglycinamidine cyclo-ligase -
  A0O31_RS03525 (A0O31_00728) - 693660..694289 (+) 630 WP_071676690.1 histidine phosphatase family protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 40013.38 Da        Isoelectric Point: 7.2368

>NTDB_id=186781 A0O31_RS03510 WP_071676687.1 690144..691229(+) (pilT) [Thermus brockianus strain GE-1]
MPKNPDIVDLLTLAVERGASDLVITVGLPPMIKVDGEFHPTEYEPLSPQDTRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPAVIKSFEELGLPKNIAEIAMAPRGLVLVTGPTGSGKSTTLASMIDYINERKPVHIV
TIEDPIEFFHRHKKAIINQREIGADTHGFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIIDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKSHQLRSVIQTGGQYGMIT
MDACLADLYKRKLITYELGLARAVDPKEFMRLAGVQEGAKR

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=186781 A0O31_RS03510 WP_071676687.1 690144..691229(+) (pilT) [Thermus brockianus strain GE-1]
ATGCCCAAGAATCCGGATATCGTGGACCTTCTGACCCTGGCCGTGGAACGGGGGGCGAGCGACCTGGTCATCACCGTAGG
GCTTCCCCCCATGATCAAGGTGGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTAAGCCCCCAGGACACCCGGCGCC
TCATGTACGCCCTGATGGACGAGAAGCAACAGCGCATCTTTGAGGAGGAGAAGGAGCTGGACTTCTCCTTTAGCCTCCCC
GGCAAAGGGCGCTACCGGGTGAACGTCTTCCTCCAGCGGGGAAGCGTGGGCGGGGTCCTCCGGGTGGTGCCCGCCGTCAT
CAAGAGCTTTGAGGAGCTGGGCCTGCCCAAGAACATCGCCGAGATCGCCATGGCCCCAAGGGGCCTGGTCCTCGTGACGG
GCCCCACGGGAAGCGGTAAGAGCACCACCTTGGCCTCCATGATCGACTACATCAACGAGCGCAAGCCGGTGCACATCGTC
ACCATTGAGGACCCCATAGAGTTTTTCCACCGCCACAAGAAGGCCATCATCAACCAGCGGGAGATCGGCGCCGACACCCA
CGGCTTCCACAAGGCCCTACGGAGCGTCCTGCGCCAGGCCCCGGACGTGATCCTGGTGGGGGAGATGCGGGACTACGAAA
CCATCGCCGCCGCCATCACCGCCGCCGAAACCGGCCACCTGGTCATGGGCACCCTGCATACCAACTCCGCCCCGGAAACC
ATTGACCGCATCATCGACGTCTTCCCGGAAAACCAGCAGGAGCAGGTGCGGGTGCAGCTTTCCAACAACCTGGTGGCCGT
GCTCACCCAGCAACTCCTCCCCAAGGCCTTTGGCGGGGGGCGGGTCCTGGCCTACGAGCTCATGATCGCCACCCCAGCGG
TGCGGGCCCTCATCCGCGAGGGCAAGAGCCACCAACTGAGGAGCGTGATCCAAACGGGGGGCCAGTACGGCATGATCACC
ATGGACGCCTGCCTGGCCGACCTCTACAAGCGCAAGCTCATCACCTACGAGCTAGGCCTCGCCCGGGCCGTGGACCCCAA
GGAGTTCATGCGCCTCGCTGGGGTGCAGGAAGGGGCGAAGCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

67.989

97.784

0.665
  pilT Acinetobacter baumannii D1279779

54.493

95.568

0.521
  pilT Acinetobacter nosocomialis M2

54.493

95.568

0.521
  pilT Acinetobacter baumannii strain A118

54.493

95.568

0.521
  pilT Acinetobacter baylyi ADP1

54.651

95.291

0.521
  pilT Pseudomonas aeruginosa PAK

54.07

95.291

0.515
  pilT Pseudomonas stutzeri DSM 10701

53.779

95.291

0.512
  pilT Neisseria meningitidis 8013

52.367

93.629

0.49
  pilT Legionella pneumophila strain ERS1305867

51.453

95.291

0.49
  pilT Legionella pneumophila strain Lp02

51.453

95.291

0.49
  pilT Vibrio cholerae strain A1552

53.191

91.136

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.191

91.136

0.485
  pilT Neisseria gonorrhoeae MS11

51.632

93.352

0.482
  pilU Pseudomonas stutzeri DSM 10701

47.23

95.014

0.449
  pilU Acinetobacter baylyi ADP1

41.457

98.892

0.41
  pilU Vibrio cholerae strain A1552

46.129

85.873

0.396

Multiple sequence alignment