Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   A0O31_RS03505 Genome accession   NZ_CP016312
Coordinates   687463..690132 (+) Length   889 a.a.
NCBI ID   WP_071676686.1    Uniprot ID   A0A1J0LTZ5
Organism   Thermus brockianus strain GE-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 682463..695132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A0O31_RS03470 (A0O31_00717) - 683466..684431 (-) 966 WP_071676679.1 LysM peptidoglycan-binding domain-containing M23 family metallopeptidase -
  A0O31_RS03475 (A0O31_00718) aroQ 684461..684898 (+) 438 WP_071676680.1 type II 3-dehydroquinate dehydratase -
  A0O31_RS03480 (A0O31_00719) crcB 684895..685266 (-) 372 WP_071676681.1 fluoride efflux transporter CrcB -
  A0O31_RS03485 (A0O31_00720) ribH 685287..685760 (+) 474 WP_071676682.1 6,7-dimethyl-8-ribityllumazine synthase -
  A0O31_RS03490 (A0O31_00721) - 685747..686151 (-) 405 WP_071676683.1 DUF4395 domain-containing protein -
  A0O31_RS03495 (A0O31_00722) pgeF 686223..686948 (+) 726 WP_071676684.1 peptidoglycan editing factor PgeF -
  A0O31_RS03500 (A0O31_00723) - 686984..687466 (+) 483 WP_071676685.1 YqeG family HAD IIIA-type phosphatase -
  A0O31_RS03505 (A0O31_00724) pilF 687463..690132 (+) 2670 WP_071676686.1 type IV pilus assembly ATPase PilB Machinery gene
  A0O31_RS03510 (A0O31_00725) pilT 690144..691229 (+) 1086 WP_071676687.1 type IV pilus twitching motility protein PilT Machinery gene
  A0O31_RS03515 (A0O31_00726) gatB 691247..692656 (+) 1410 WP_071676688.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  A0O31_RS03520 (A0O31_00727) purM 692662..693663 (+) 1002 WP_071676689.1 phosphoribosylformylglycinamidine cyclo-ligase -
  A0O31_RS03525 (A0O31_00728) - 693660..694289 (+) 630 WP_071676690.1 histidine phosphatase family protein -

Sequence

Protein


Download         Length: 889 a.a.        Molecular weight: 97875.86 Da        Isoelectric Point: 5.1698

>NTDB_id=186780 A0O31_RS03505 WP_071676686.1 687463..690132(+) (pilF) [Thermus brockianus strain GE-1]
MSVLTIGDKRLGAILLDAGLLTDEELQLALEKHREVGGSLAEVIVESGLLSERRIAQAIEDHFGIPLVELHQLEIPPKVK
ALLPAEKAKELGAIPFALDEEAGVVRVAFVNPLDTLALEEVEDLTGLVVEPYQTTKSAFQYALAKNYPELGLPLPPPPTG
PSQGELRLGEFLLEKGLIDRETLEEALVEQERTGDLLGQILVRKGLSEEVLYRTLAEQKGLEFLPSTEGLTPDPAATALL
LRSDALRYSAVPVAIREGKVEVVLADPRHKQAVEELLGRPARFYLTLSKAWEALFHQAYPEKGRIGEVLVQEGRLSREHL
REALEVQKRLPKAKPLGEILVELGLARPEDVEEALKKQRQGGGRLEDTLVQSGKLKPEALAQAVAHQLGYPYIDPQENPP
DPGAALLLPEDLARRYGVFPHHLEGKTLVLLMKDPRNILALDDVRLALKRKGLGYEVTPAVATEAAIVKLIERFYGKEEL
GELAKELSKGYQAEEEALTTELDESAAQRFVKQVIREAYLQDASDIHIEPRQSDVLVRLRIDGTLRQYTTLSKGALGPVI
SVIKIMGGLNIAEKRLPQDGRVRYREGAIDADLRLSTLPTVYGEKAVMRLLKKAADIPEIEGLGFAPGVFERFQEVISKP
YGIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIR
DPETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVELFNISAALIGVLSQRLVRRICEHCKVEVKPDPEVLRRLGI
PEEEMQGAKLYKGTGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARKKGMKTLREDGVYKALQGITTL
EEVLARTIE

Nucleotide


Download         Length: 2670 bp        

>NTDB_id=186780 A0O31_RS03505 WP_071676686.1 687463..690132(+) (pilF) [Thermus brockianus strain GE-1]
ATGAGCGTGCTGACCATTGGCGACAAGCGGCTTGGGGCCATCCTTTTGGACGCCGGGCTCCTCACGGACGAGGAACTCCA
GCTGGCCCTGGAGAAGCACCGGGAGGTGGGGGGAAGCCTGGCGGAGGTCATCGTGGAATCGGGCCTCCTCTCGGAAAGGC
GCATCGCCCAGGCCATTGAGGACCACTTCGGCATCCCCCTGGTGGAACTCCACCAGCTGGAAATCCCCCCGAAGGTGAAG
GCCCTATTACCGGCGGAGAAGGCCAAGGAGCTGGGCGCCATCCCCTTTGCCCTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCGTGAACCCCTTGGACACCTTGGCCCTCGAGGAGGTGGAAGACCTCACGGGCCTGGTGGTGGAGCCCTACCAAA
CCACCAAGAGCGCCTTCCAGTACGCCCTGGCCAAGAACTACCCTGAGCTCGGCCTACCCCTTCCGCCCCCACCCACGGGG
CCAAGCCAAGGGGAACTCCGCCTAGGGGAGTTCCTTCTGGAAAAGGGCCTCATTGACCGGGAAACCTTGGAAGAGGCCTT
GGTGGAACAGGAGCGGACGGGGGACCTCTTAGGCCAGATCCTGGTGCGAAAGGGGCTTTCCGAGGAGGTCCTCTATCGGA
CCCTGGCCGAGCAAAAGGGGCTAGAATTCCTGCCCTCCACCGAGGGGCTAACCCCCGACCCCGCCGCCACCGCCCTCCTC
CTGCGCTCGGATGCCCTGCGCTATAGCGCCGTTCCCGTGGCCATCCGCGAGGGCAAAGTGGAGGTGGTCCTGGCCGACCC
CCGGCACAAGCAGGCGGTGGAGGAGCTTCTGGGCCGCCCGGCCCGTTTCTACCTCACCCTGTCCAAGGCCTGGGAAGCCC
TCTTCCACCAGGCCTACCCAGAGAAGGGCCGTATAGGAGAGGTCTTGGTTCAGGAAGGCCGCCTGAGCCGGGAACACCTG
CGGGAAGCCTTGGAGGTGCAAAAGCGCCTCCCCAAAGCGAAGCCCTTGGGGGAGATCCTGGTGGAGCTTGGCCTGGCCCG
CCCCGAGGACGTGGAGGAGGCCCTCAAGAAGCAACGCCAAGGGGGAGGCCGCCTGGAAGACACCTTGGTCCAGTCGGGCA
AGCTCAAGCCCGAGGCCCTGGCCCAGGCGGTGGCCCACCAGCTGGGCTACCCCTATATTGACCCCCAGGAAAATCCTCCA
GACCCCGGCGCCGCCCTCCTCCTCCCCGAGGACCTAGCCCGGCGCTACGGCGTCTTCCCCCACCACCTGGAGGGGAAAAC
CTTGGTCCTTCTCATGAAGGACCCCCGGAACATCCTGGCCCTGGACGACGTGCGCCTGGCCTTGAAGCGGAAGGGCCTGG
GGTACGAGGTGACCCCCGCCGTGGCCACCGAGGCGGCCATCGTCAAGCTCATTGAGCGCTTCTACGGCAAGGAGGAGCTG
GGCGAGCTGGCCAAGGAGCTCTCCAAGGGGTACCAGGCGGAGGAAGAAGCCCTCACCACGGAGCTGGACGAAAGCGCCGC
CCAGCGCTTCGTCAAGCAGGTGATCCGGGAGGCCTACCTTCAGGACGCCTCCGACATTCACATAGAACCCCGCCAGTCGG
ACGTCCTGGTGCGCCTACGCATTGACGGTACCCTGCGCCAATACACCACCTTGTCTAAAGGGGCCTTGGGCCCGGTGATC
AGCGTCATCAAGATCATGGGCGGGCTCAACATCGCGGAAAAGCGCCTCCCCCAGGACGGCCGCGTCCGCTACCGGGAAGG
GGCCATTGACGCGGACCTCAGGCTTTCCACCCTCCCCACGGTCTACGGGGAAAAGGCGGTGATGCGCCTTTTGAAGAAGG
CGGCGGACATCCCCGAGATAGAGGGGCTGGGCTTCGCCCCCGGGGTCTTTGAGCGCTTCCAGGAAGTGATCTCCAAGCCC
TACGGCATCTTCCTCATCACCGGCCCCACGGGAAGCGGCAAGAGCTTCACCACCTTCTCCATCCTGAAGCGCATCGCCAC
CCCCGACAAGAACACCCAGACCATTGAAGACCCCGTGGAGTACGAGATCCCCGGCATCAACCAGACCCAGGTGAACCCCC
AAGCCGGGCTCACCTTCGCCCGGGCCTTGCGCGCCTTCCTGCGCCAGGACCCGGACATCATCATGGTGGGGGAGATCCGC
GACCCGGAGACCGCCAAGATCGCCACGGAGGCGGCCCTCACCGGCCACCTGGTCATCGCTACCCTGCACACCAACGACGC
CGCCCAGGCCATCACCCGCTTGGACGAAATGGGGGTGGAGCTTTTCAACATCTCCGCCGCCCTCATCGGCGTCCTTTCCC
AGCGCTTGGTGCGCAGGATCTGCGAGCACTGCAAGGTGGAGGTGAAGCCCGACCCCGAGGTCCTCCGCCGGCTCGGTATC
CCCGAGGAGGAGATGCAAGGAGCAAAGCTCTACAAGGGCACGGGGTGCGAGCGCTGCGGGGGCACGGGGTACAAGGGCCG
CTACGCCATCCACGAGCTGTTGGTGGTGGACGACGAGATCCGGCACGCCATCGTGGCGGGCAAGTCGGCCACGGAAATCA
AGGAGATCGCCCGCAAGAAGGGCATGAAGACCTTGCGGGAAGACGGCGTCTACAAGGCCCTCCAGGGGATTACCACCCTC
GAGGAGGTCCTGGCGCGTACCATTGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(513-779)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-148)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(201-303)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(369-479)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J0LTZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

86.502

100

0.865
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.18

100

0.562

Multiple sequence alignment