Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   dqs_RS17805 Genome accession   NZ_CP016210
Coordinates   3890964..3892004 (+) Length   346 a.a.
NCBI ID   WP_011767190.1    Uniprot ID   -
Organism   Azoarcus olearius strain DQS-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3885964..3897004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  dqs_RS17775 (dqs_3605) - 3886005..3886964 (+) 960 WP_011767184.1 aspartate carbamoyltransferase catalytic subunit -
  dqs_RS17780 (dqs_3606) - 3887035..3888318 (+) 1284 WP_065341293.1 dihydroorotase -
  dqs_RS17785 (dqs_3607) - 3888325..3888621 (-) 297 WP_011767186.1 DUF167 domain-containing protein -
  dqs_RS17790 (dqs_3608) - 3888626..3889207 (-) 582 WP_011767187.1 YggT family protein -
  dqs_RS17795 (dqs_3609) proC 3889226..3890050 (-) 825 WP_065341294.1 pyrroline-5-carboxylate reductase -
  dqs_RS17800 (dqs_3610) - 3890124..3890837 (-) 714 WP_065341295.1 YggS family pyridoxal phosphate-dependent enzyme -
  dqs_RS17805 (dqs_3611) pilT 3890964..3892004 (+) 1041 WP_011767190.1 type IV pilus twitching motility protein PilT Machinery gene
  dqs_RS17810 (dqs_3612) pilU 3892091..3893224 (+) 1134 WP_011767191.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  dqs_RS17815 (dqs_3613) - 3893413..3896130 (+) 2718 WP_065341296.1 type VI secretion system Vgr family protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38473.05 Da        Isoelectric Point: 7.0158

>NTDB_id=186201 dqs_RS17805 WP_011767190.1 3890964..3892004(+) (pilT) [Azoarcus olearius strain DQS-4]
MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEHKDVHAMVYDIMNDGQRKQFEESWECDFSFAVPNLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEELNCPKIFKDIANQPRGIVLVTGPTGSGKSTTLAAMVDYINENEYGHILTVED
PIEFVHESKRCLINQREVHRDTQSFNNALRASLREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGSGRVAAHEIMIGSPAIRNLIRENKIAQMYSAIQTGQNLGMQTLDQC
LADLVRRNVVSAAEARVRAQNKDNFA

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=186201 dqs_RS17805 WP_011767190.1 3890964..3892004(+) (pilT) [Azoarcus olearius strain DQS-4]
ATGGACATCACCGAACTGCTCGCCTTCGCGGTCAAGAACAAGGCTTCCGACCTGCATCTCTCCGCCGGGCTGCCGCCCAT
GATCCGGGTGCACGGCGACGTGCGCCGCATCAATCTGCCGCCCATGGAGCACAAGGACGTGCACGCCATGGTGTACGACA
TCATGAACGACGGCCAGCGCAAGCAGTTCGAGGAAAGCTGGGAGTGCGACTTCTCGTTCGCGGTGCCCAATCTGGCCCGC
TTCCGGGTCAACGCGTTCAACCAGAACCGTGGCGCCGGCGCCGTGTTCCGGACCATTCCGTCCAAGGTGCTGACGCTCGA
AGAACTGAACTGCCCGAAGATCTTCAAGGACATCGCGAACCAGCCGCGCGGCATCGTGCTGGTCACCGGGCCGACGGGCT
CGGGCAAATCAACCACGCTCGCGGCGATGGTGGACTACATCAACGAGAACGAATACGGCCACATCCTCACGGTCGAGGAC
CCGATCGAATTCGTCCATGAATCCAAGCGCTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCAGTCGTTCAACAA
CGCGCTGCGCGCTTCCCTGCGCGAAGATCCGGACGTGATCCTGGTTGGCGAAATGCGCGACCTCGAAACCATCCGTCTCG
CGCTCACCGCGGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCGGCCGCGGAAAAGGACATGGTGCGCTCGATGCTTTCCGAGTCGCTGCGCGCTGTCATCTCGCAGAC
CCTGCTGAAGACCAAGGACGGCAGCGGTCGCGTGGCCGCCCACGAGATCATGATCGGCTCGCCCGCCATCCGTAACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGAATCTGGGCATGCAGACGCTGGACCAGTGC
CTCGCCGACCTCGTCCGCCGCAACGTCGTCTCCGCGGCAGAAGCGCGCGTGCGTGCGCAGAACAAGGACAACTTCGCCTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.29

99.711

0.801
  pilT Acinetobacter baumannii strain A118

80.29

99.711

0.801
  pilT Acinetobacter nosocomialis M2

80.29

99.711

0.801
  pilT Pseudomonas aeruginosa PAK

77.681

99.711

0.775
  pilT Acinetobacter baylyi ADP1

76.812

99.711

0.766
  pilT Pseudomonas stutzeri DSM 10701

76.522

99.711

0.763
  pilT Legionella pneumophila strain Lp02

72.754

99.711

0.725
  pilT Legionella pneumophila strain ERS1305867

72.754

99.711

0.725
  pilT Neisseria meningitidis 8013

70.809

100

0.708
  pilT Neisseria gonorrhoeae MS11

70.52

100

0.705
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.235

98.266

0.671
  pilT Vibrio cholerae strain A1552

68.235

98.266

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.757

100

0.538
  pilU Acinetobacter baylyi ADP1

41.667

100

0.419
  pilU Vibrio cholerae strain A1552

42.985

96.821

0.416
  pilU Pseudomonas stutzeri DSM 10701

41.159

99.711

0.41

Multiple sequence alignment