Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A9404_RS06690 Genome accession   NZ_CP016027
Coordinates   1500924..1501973 (+) Length   349 a.a.
NCBI ID   WP_066099474.1    Uniprot ID   A0A191ZGY3
Organism   Halothiobacillus diazotrophicus strain LS2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1495924..1506973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9404_RS06665 (A9404_06665) metW 1496622..1497224 (-) 603 WP_066099470.1 methionine biosynthesis protein MetW -
  A9404_RS06670 (A9404_06670) - 1497221..1498342 (-) 1122 WP_322098977.1 homoserine O-acetyltransferase -
  A9404_RS06675 (A9404_06675) - 1498524..1499075 (-) 552 WP_066099471.1 YggT family protein -
  A9404_RS06680 (A9404_06680) proC 1499072..1499923 (-) 852 WP_066099472.1 pyrroline-5-carboxylate reductase -
  A9404_RS06685 (A9404_06685) - 1499978..1500682 (-) 705 WP_066099473.1 YggS family pyridoxal phosphate-dependent enzyme -
  A9404_RS06690 (A9404_06690) pilT 1500924..1501973 (+) 1050 WP_066099474.1 type IV pilus twitching motility protein PilT Machinery gene
  A9404_RS06695 (A9404_06695) pilU 1502022..1503167 (+) 1146 WP_066099475.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A9404_RS06700 (A9404_06700) - 1503288..1504448 (+) 1161 WP_066099476.1 PilT/PilU family type 4a pilus ATPase -
  A9404_RS06710 (A9404_06710) thiO 1504766..1505869 (+) 1104 WP_197490290.1 glycine oxidase ThiO -
  A9404_RS06715 (A9404_06715) - 1505893..1506231 (-) 339 WP_066099478.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38840.53 Da        Isoelectric Point: 7.3031

>NTDB_id=185421 A9404_RS06690 WP_066099474.1 1500924..1501973(+) (pilT) [Halothiobacillus diazotrophicus strain LS2]
MAEQRGIDSLLNFSVKNGASDLHLSAGEQPRVRIDGDIRKVNVPVMEHKEVHAMVYDIMNDKQRKEYEDNLEVDFSFELP
GVARFRVNAFNQNRGAAAVFRTIPSEILSLEQLAAPNVFKDLCLFPRGIVLVTGPTGSGKSTTLAAMINHINENRNDHIL
TIEDPIEFVHQSKKCLINQREVHRDTHSFNAALRSALREDPDVVLVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKT
IDRVVDVFPAAEKDMVRAMLSESLRAVISQTLLKKIGGGRMAAHEIMLGTPAIRNLIRENKIAQMYSAIQTGQTQGMQTL
DQNLLQLVKRGLIAKDEARARAQNKNDFM

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=185421 A9404_RS06690 WP_066099474.1 1500924..1501973(+) (pilT) [Halothiobacillus diazotrophicus strain LS2]
ATGGCAGAACAACGTGGCATTGATTCTCTCCTCAATTTCTCCGTAAAGAACGGCGCGTCGGATCTGCACCTCTCCGCGGG
CGAGCAACCGCGCGTACGGATTGACGGCGACATTCGGAAAGTGAACGTCCCCGTGATGGAGCACAAGGAAGTGCACGCCA
TGGTGTACGACATCATGAACGACAAGCAGCGCAAGGAGTACGAGGACAATCTCGAAGTCGACTTCTCCTTCGAACTGCCT
GGCGTCGCGCGTTTCCGTGTCAACGCCTTCAACCAGAATCGCGGCGCCGCGGCCGTATTCCGGACGATTCCCAGCGAGAT
CCTGAGCCTGGAACAGCTCGCCGCGCCGAATGTTTTCAAGGATCTTTGCCTGTTCCCCCGTGGCATCGTTCTGGTCACCG
GACCGACCGGTTCCGGTAAGTCGACCACGCTCGCAGCCATGATCAACCACATCAACGAGAATCGGAACGACCACATTCTG
ACGATCGAAGACCCGATCGAATTCGTGCATCAGAGCAAGAAGTGCCTGATCAACCAACGGGAAGTCCATCGGGATACCCA
CAGTTTCAATGCCGCGCTGCGATCTGCACTGCGTGAAGACCCGGACGTCGTGCTCGTCGGCGAGATGCGCGATCTGGAAA
CCATTCGCCTGGCGTTGACCGCCGCCGAAACGGGCCACCTCGTTTTCGGCACGCTGCACACCAGTTCTGCCGCCAAAACC
ATCGACCGGGTTGTCGACGTCTTCCCCGCCGCCGAAAAGGACATGGTGCGCGCCATGCTGTCGGAGTCGCTGCGCGCCGT
CATTTCGCAAACCCTGCTCAAGAAAATCGGCGGCGGCCGGATGGCAGCCCACGAAATCATGCTGGGCACACCCGCAATTC
GAAATCTGATTCGCGAAAACAAGATCGCCCAGATGTACTCAGCCATCCAGACCGGCCAGACCCAAGGGATGCAAACCCTC
GACCAGAACCTACTGCAACTGGTCAAGCGCGGGTTGATCGCCAAGGACGAAGCTCGGGCGCGCGCGCAAAACAAGAACGA
TTTCATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A191ZGY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.259

98.281

0.759
  pilT Acinetobacter baumannii D1279779

77.259

98.281

0.759
  pilT Acinetobacter baumannii strain A118

77.259

98.281

0.759
  pilT Pseudomonas aeruginosa PAK

73.977

97.994

0.725
  pilT Acinetobacter baylyi ADP1

73.392

97.994

0.719
  pilT Pseudomonas stutzeri DSM 10701

73.099

97.994

0.716
  pilT Legionella pneumophila strain Lp02

71.637

97.994

0.702
  pilT Legionella pneumophila strain ERS1305867

71.637

97.994

0.702
  pilT Neisseria meningitidis 8013

70.796

97.135

0.688
  pilT Neisseria gonorrhoeae MS11

70.501

97.135

0.685
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

96.562

0.679
  pilT Vibrio cholerae strain A1552

70.326

96.562

0.679
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.711

99.14

0.493
  pilU Pseudomonas stutzeri DSM 10701

41.399

98.281

0.407
  pilU Acinetobacter baylyi ADP1

39.535

98.567

0.39
  pilU Vibrio cholerae strain A1552

39.359

98.281

0.387

Multiple sequence alignment