Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   A9404_RS06695 Genome accession   NZ_CP016027
Coordinates   1502022..1503167 (+) Length   381 a.a.
NCBI ID   WP_066099475.1    Uniprot ID   A0A191ZGX9
Organism   Halothiobacillus diazotrophicus strain LS2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1497022..1508167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9404_RS06670 (A9404_06670) - 1497221..1498342 (-) 1122 WP_322098977.1 homoserine O-acetyltransferase -
  A9404_RS06675 (A9404_06675) - 1498524..1499075 (-) 552 WP_066099471.1 YggT family protein -
  A9404_RS06680 (A9404_06680) proC 1499072..1499923 (-) 852 WP_066099472.1 pyrroline-5-carboxylate reductase -
  A9404_RS06685 (A9404_06685) - 1499978..1500682 (-) 705 WP_066099473.1 YggS family pyridoxal phosphate-dependent enzyme -
  A9404_RS06690 (A9404_06690) pilT 1500924..1501973 (+) 1050 WP_066099474.1 type IV pilus twitching motility protein PilT Machinery gene
  A9404_RS06695 (A9404_06695) pilU 1502022..1503167 (+) 1146 WP_066099475.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A9404_RS06700 (A9404_06700) - 1503288..1504448 (+) 1161 WP_066099476.1 PilT/PilU family type 4a pilus ATPase -
  A9404_RS06710 (A9404_06710) thiO 1504766..1505869 (+) 1104 WP_197490290.1 glycine oxidase ThiO -
  A9404_RS06715 (A9404_06715) - 1505893..1506231 (-) 339 WP_066099478.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42637.60 Da        Isoelectric Point: 6.0977

>NTDB_id=185422 A9404_RS06695 WP_066099475.1 1502022..1503167(+) (pilU) [Halothiobacillus diazotrophicus strain LS2]
MDRQQAANYVQGLLRKAVELEASDLFVTVGAPPSAKINGEITHLADTSLSTSHTQMLVRSIMNDKQIASFEETREANFAI
SLPGIARFRVNAFMQRGSVGMVLRMIRSDVPKFSELLLPKILEDISMTKRGLVIFVGATSSGKSTSLAAMLDYRNEHSAG
HIVTIEDPIEYVHRHKKCLVTQREVGVDTDSFEAALKNTLRQAPDVILIGEIRDRDTMDHAIAFAETGHLCLATLHANNT
NQALDRIINFFPEERRQQLLMDLSLNLKSVISQRLLRKEEGSGRVPAVEVLINSPLIGDLIFKGDVHEIKEVMKRSRELG
MQTFDQALLDLYDSGKVSFQEALRNADSQNDLRLNIKLHSNRGLPKDHDEDDDGLRVEKDD

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=185422 A9404_RS06695 WP_066099475.1 1502022..1503167(+) (pilU) [Halothiobacillus diazotrophicus strain LS2]
ATGGATCGTCAACAAGCGGCCAACTACGTCCAGGGATTGCTCCGAAAGGCAGTCGAACTGGAAGCCTCAGATCTGTTCGT
CACCGTCGGCGCACCGCCGAGCGCCAAGATCAACGGTGAAATCACGCACCTTGCCGATACGAGCCTGTCCACCAGCCACA
CGCAAATGCTGGTGCGCTCCATCATGAATGACAAGCAGATTGCCTCGTTCGAGGAAACCCGCGAGGCGAACTTCGCCATT
TCACTGCCGGGTATCGCACGATTCCGCGTCAATGCGTTCATGCAACGAGGCAGTGTCGGTATGGTCCTGCGGATGATCCG
TTCCGACGTCCCGAAGTTTTCCGAGCTGCTTCTCCCCAAGATCCTGGAAGACATCTCGATGACGAAACGCGGGCTCGTCA
TCTTCGTCGGGGCGACGAGTTCGGGTAAATCGACCTCCCTGGCCGCCATGCTTGATTACCGCAACGAACATTCCGCCGGT
CACATCGTGACCATCGAAGATCCCATCGAATACGTGCATCGGCATAAGAAGTGTCTCGTGACGCAACGAGAGGTCGGGGT
CGACACGGATAGCTTCGAGGCCGCCCTCAAGAACACGCTGCGGCAGGCGCCGGACGTCATCCTCATTGGCGAGATCCGGG
ATCGAGACACCATGGATCACGCCATTGCGTTCGCGGAAACGGGCCACCTGTGTCTGGCAACGCTCCACGCCAACAACACC
AACCAGGCCCTGGACCGCATCATCAACTTCTTCCCAGAGGAACGTCGCCAGCAACTGCTGATGGATCTGTCTCTGAACCT
CAAGTCCGTCATCTCACAGCGACTGCTGCGCAAGGAGGAAGGCTCCGGTCGGGTGCCCGCCGTCGAGGTACTGATCAACT
CCCCCCTGATCGGCGACCTGATCTTCAAGGGGGATGTGCATGAGATCAAGGAAGTCATGAAACGCTCCCGAGAACTCGGC
ATGCAGACCTTCGACCAAGCCCTGCTGGATCTTTACGACTCCGGGAAAGTCAGCTTTCAGGAAGCCTTGCGCAATGCCGA
CTCGCAAAACGATCTACGACTGAATATCAAGCTGCACAGCAACCGCGGTCTCCCGAAGGACCACGACGAGGACGACGACG
GACTGCGTGTAGAAAAGGACGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A191ZGX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.946

97.638

0.585
  pilU Acinetobacter baylyi ADP1

58.774

94.226

0.554
  pilU Vibrio cholerae strain A1552

56.18

93.438

0.525
  pilT Acinetobacter baylyi ADP1

45.238

88.189

0.399
  pilT Acinetobacter baumannii D1279779

44.807

88.451

0.396
  pilT Acinetobacter nosocomialis M2

44.807

88.451

0.396
  pilT Acinetobacter baumannii strain A118

44.807

88.451

0.396
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.91

87.664

0.394
  pilT Pseudomonas aeruginosa PAK

44.012

87.664

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.413

87.664

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.739

90.551

0.378
  pilT Vibrio cholerae strain A1552

41.739

90.551

0.378
  pilT Neisseria meningitidis 8013

40.762

89.501

0.365

Multiple sequence alignment