Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   A3K93_RS02730 Genome accession   NZ_CP015594
Coordinates   586061..587182 (-) Length   373 a.a.
NCBI ID   WP_067728710.1    Uniprot ID   A0A172YM85
Organism   Acinetobacter sp. NCu2D-2     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 581061..592182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3K93_RS02700 (A3K93_02700) - 581817..582665 (+) 849 WP_067728697.1 class II glutamine amidotransferase -
  A3K93_RS02705 (A3K93_02705) - 582936..583391 (+) 456 WP_067728699.1 bacteriohemerythrin -
  A3K93_RS02710 (A3K93_02710) - 583534..584613 (+) 1080 WP_067728701.1 hypothetical protein -
  A3K93_RS02715 (A3K93_02715) - 584613..584957 (+) 345 WP_067728703.1 RnfH family protein -
  A3K93_RS02720 (A3K93_02720) bamE 585046..585444 (-) 399 WP_067728705.1 outer membrane protein assembly factor BamE -
  A3K93_RS02725 (A3K93_02725) fur 585557..585994 (+) 438 WP_067728707.1 ferric iron uptake transcriptional regulator -
  A3K93_RS02730 (A3K93_02730) pilU 586061..587182 (-) 1122 WP_067728710.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A3K93_RS02735 (A3K93_02735) pilT 587209..588246 (-) 1038 WP_067728712.1 type IV pilus twitching motility protein PilT Machinery gene
  A3K93_RS02740 (A3K93_02740) - 588369..589061 (+) 693 WP_067728714.1 YggS family pyridoxal phosphate-dependent enzyme -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41622.75 Da        Isoelectric Point: 6.7812

>NTDB_id=181235 A3K93_RS02730 WP_067728710.1 586061..587182(-) (pilU) [Acinetobacter sp. NCu2D-2]
MDFNGLLDYMIEKKASDLFVTADVEPSIKINGQIHPIGSVKLPGATVGQLLHSIMSDKQRKEFAETRECNFAISNASQTA
RFRVSAFQQRDQPGMVLRRIETTIPTMDELKLPPVLKELAMTKRGIIIFVGATGTGKSTSLASLIGYRNENSKGHIITIE
DPIEFVHQHKGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLKAMVAQQLIPTIDGTGRRAAIEILINSPLISDLIRKGDVHEIKEIMKRSRELGMQTFDQ
ALFDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGGSHLLNANSNITFDGQT

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=181235 A3K93_RS02730 WP_067728710.1 586061..587182(-) (pilU) [Acinetobacter sp. NCu2D-2]
ATGGATTTTAACGGCTTACTTGACTACATGATAGAAAAGAAAGCATCGGACTTATTCGTGACAGCCGATGTTGAACCTTC
TATTAAAATTAATGGTCAAATCCATCCAATTGGTTCAGTCAAGTTACCAGGTGCAACAGTTGGACAATTGTTGCACTCCA
TCATGAGTGATAAGCAGCGTAAAGAATTTGCTGAAACACGTGAGTGTAACTTTGCGATCAGTAATGCCAGTCAAACTGCG
CGTTTCCGTGTCAGTGCATTTCAGCAACGTGACCAACCGGGTATGGTGTTACGTCGAATTGAAACCACTATTCCAACCAT
GGATGAGCTAAAGCTACCGCCTGTACTGAAAGAATTGGCGATGACTAAACGCGGTATTATCATTTTCGTAGGTGCAACAG
GTACAGGTAAATCAACCTCCCTTGCCTCTTTAATTGGCTATCGCAATGAAAACTCAAAAGGTCATATCATTACCATTGAA
GATCCAATTGAATTTGTACACCAACACAAGGGTTGCATCATTACTCAGCGTGAAGTCGGTATCGATACCGATTCATTTGA
AGTCGCACTAAAAAATACGCTGCGTCAGGCACCTGATGTAATTTTGATTGGTGAGATTCGTTCACGCGAAACCATGGACT
ACGCGATTGCCTTTGCCGAAACAGGTCACTTGGTCTTTGCCACCCTGCATGCGAACAACGCCAACCAAGCAATTGATCGT
ATTATTCACTTCTTTGAAGCTGACCGCCATAGTCAATTGTTTATGGATTTATCGCTGAACTTAAAAGCGATGGTCGCACA
GCAATTGATTCCAACCATTGATGGTACAGGTCGTCGTGCTGCGATTGAAATCCTGATTAACTCTCCATTAATTTCTGATC
TCATCCGTAAAGGTGATGTGCATGAAATTAAAGAGATTATGAAGCGCTCACGCGAGTTGGGCATGCAAACCTTCGACCAA
GCATTGTTTGATCTGTATAAAGCAAAACAAATCACGTACAAGGACGCGCTTAAACACGCGGATTCTCCTAACGATTTGCG
TCTTCAAATCAAACTTTCCGAAGAAGGTGGTTCACATCTGCTCAATGCCAATTCGAATATTACGTTCGACGGGCAAACCT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172YM85

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

72.751

100

0.737
  pilU Pseudomonas stutzeri DSM 10701

68.194

99.464

0.678
  pilU Vibrio cholerae strain A1552

55.946

99.196

0.555
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.064

92.761

0.399
  pilT Legionella pneumophila strain Lp02

43.155

90.08

0.389
  pilT Legionella pneumophila strain ERS1305867

43.155

90.08

0.389
  pilT Acinetobacter nosocomialis M2

41.691

91.957

0.383
  pilT Acinetobacter baumannii D1279779

41.691

91.957

0.383
  pilT Acinetobacter baumannii strain A118

41.691

91.957

0.383
  pilT Pseudomonas aeruginosa PAK

41.329

92.761

0.383
  pilT Acinetobacter baylyi ADP1

40.525

91.957

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.601

94.102

0.373
  pilT Vibrio cholerae strain A1552

39.601

94.102

0.373
  pilT Neisseria gonorrhoeae MS11

40.173

92.761

0.373
  pilT Neisseria meningitidis 8013

40.173

92.761

0.373
  pilT Pseudomonas stutzeri DSM 10701

40.173

92.761

0.373

Multiple sequence alignment