Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AYJ58_RS09655 Genome accession   NZ_CP015194
Coordinates   2173311..2174348 (+) Length   345 a.a.
NCBI ID   WP_006082517.1    Uniprot ID   A0AA50Q7P7
Organism   Shewanella sp. Pdp11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2168311..2179348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYJ58_RS09625 (AYJ58_09465) rdgB 2169326..2169943 (-) 618 WP_106649926.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  AYJ58_RS09630 (AYJ58_09470) - 2170083..2170517 (-) 435 WP_106649927.1 DUF4426 domain-containing protein -
  AYJ58_RS09635 (AYJ58_09475) yggU 2170643..2170942 (-) 300 WP_106649928.1 DUF167 family protein YggU -
  AYJ58_RS09640 (AYJ58_09480) - 2170942..2171490 (-) 549 WP_106649929.1 YggT family protein -
  AYJ58_RS09645 (AYJ58_09485) proC 2171544..2172362 (-) 819 WP_106649930.1 pyrroline-5-carboxylate reductase -
  AYJ58_RS09650 (AYJ58_09490) - 2172577..2173275 (-) 699 WP_106651795.1 YggS family pyridoxal phosphate-dependent enzyme -
  AYJ58_RS09655 (AYJ58_09495) pilT 2173311..2174348 (+) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  AYJ58_RS09660 (AYJ58_09500) pilU 2174358..2175470 (+) 1113 WP_106649931.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AYJ58_RS09665 (AYJ58_09505) - 2175658..2176197 (+) 540 WP_106649932.1 glutathione peroxidase -
  AYJ58_RS09670 (AYJ58_09510) hemH 2176230..2177243 (+) 1014 WP_106649933.1 ferrochelatase -
  AYJ58_RS09675 (AYJ58_09515) ruvX 2177330..2177755 (-) 426 WP_006082513.1 Holliday junction resolvase RuvX -
  AYJ58_RS09680 (AYJ58_09520) - 2177785..2178348 (-) 564 WP_106649934.1 YqgE/AlgH family protein -
  AYJ58_RS09685 (AYJ58_09525) yciH 2178415..2178744 (-) 330 WP_106649935.1 stress response translation initiation inhibitor YciH -
  AYJ58_RS09690 (AYJ58_09530) - 2178923..2179240 (-) 318 WP_106649936.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38297.94 Da        Isoelectric Point: 6.9095

>NTDB_id=177392 AYJ58_RS09655 WP_006082517.1 2173311..2174348(+) (pilT) [Shewanella sp. Pdp11]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=177392 AYJ58_RS09655 WP_006082517.1 2173311..2174348(+) (pilT) [Shewanella sp. Pdp11]
ATGGAAATCACTGAGTTATTGGCATTTAGTGTAAAACACAAAGCCTCGGATCTACACCTTTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAAATTAACCTGCCCGCGCTCGATCACCAAGGCGTACACAGCCTAGTGTACGACA
TAATGAATGATAAGCAGCGCAAGGACTACGAAGAACATCTAGAAATCGATTTCTCGTTCGAAGTCCCTAATCTTGCCCGC
TTTCGTGTTAACGCATTCAATCAATCCCGTGGTGCTGCGGCCGTATTTCGTACCATTCCTAGCGACATCTTGTCACTTGA
GCAATTAGGTGCACCGGAGATTTTTAAAAAAATCGCCAGTTTCCCTCGGGGATTAGTGCTAGTGACTGGGCCAACGGGTT
CGGGTAAGAGTACCACACTTGCCGCTATGGTGGATTACGTTAACGAAAATCGACATGATCACATCTTAACCATTGAAGAT
CCTATCGAATTCGTACACCAAAATAAGCAATGTTTGATTAACCAGCGAGAAGTTCACCGCCATACCCACAGCTTTAATGC
GGCGCTTAGAAGTGCACTACGTGAAGATCCGGATGTGATTTTAGTCGGTGAGATGCGTGACCTTGAAACTATTCGTTTGG
CTATGACTGCCGCTGAAACAGGCCACTTAGTGTTTGGTACCTTGCATACCACCTCAGCCGCGAAAACCATTGACCGTGTG
GTCGACGTGTTCCCCGCCGGTGAAAAAGACATGGTGCGCACTATGTTGTCGGAATCGCTGCAAGCGGTTATTTCACAAAC
CCTGATTAAAAAAGTCGGCGGAGGCCGTGTTGCCGCCCATGAAATCATGATGGGCACGCCCGCTATTCGTAACCTTATTC
GTGAAGACAAAGTGGCGCAGATGTATTCGGCGATTCAAACCGGGATGGCCCACGGAATGCAGACCTTAGAGCAATGTTTA
CAGAACTTAGTCAATCGCGGTCTTATTACCCGTGAAGATGCAATGGCGAAGAGTTCGAATAAACAAGCGACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.409

99.13

0.797
  pilT Acinetobacter baumannii D1279779

80.409

99.13

0.797
  pilT Acinetobacter baumannii strain A118

80.409

99.13

0.797
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas aeruginosa PAK

78.698

97.971

0.771
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.294

97.681

0.716
  pilT Vibrio cholerae strain A1552

73.294

97.681

0.716
  pilT Neisseria meningitidis 8013

69.767

99.71

0.696
  pilT Neisseria gonorrhoeae MS11

69.477

99.71

0.693
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.558

98.261

0.487
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.399

99.42

0.412
  pilU Acinetobacter baylyi ADP1

39.763

97.681

0.388

Multiple sequence alignment