Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AYJ58_RS09660 Genome accession   NZ_CP015194
Coordinates   2174358..2175470 (+) Length   370 a.a.
NCBI ID   WP_106649931.1    Uniprot ID   -
Organism   Shewanella sp. Pdp11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2169358..2180470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYJ58_RS09630 (AYJ58_09470) - 2170083..2170517 (-) 435 WP_106649927.1 DUF4426 domain-containing protein -
  AYJ58_RS09635 (AYJ58_09475) yggU 2170643..2170942 (-) 300 WP_106649928.1 DUF167 family protein YggU -
  AYJ58_RS09640 (AYJ58_09480) - 2170942..2171490 (-) 549 WP_106649929.1 YggT family protein -
  AYJ58_RS09645 (AYJ58_09485) proC 2171544..2172362 (-) 819 WP_106649930.1 pyrroline-5-carboxylate reductase -
  AYJ58_RS09650 (AYJ58_09490) - 2172577..2173275 (-) 699 WP_106651795.1 YggS family pyridoxal phosphate-dependent enzyme -
  AYJ58_RS09655 (AYJ58_09495) pilT 2173311..2174348 (+) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  AYJ58_RS09660 (AYJ58_09500) pilU 2174358..2175470 (+) 1113 WP_106649931.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AYJ58_RS09665 (AYJ58_09505) - 2175658..2176197 (+) 540 WP_106649932.1 glutathione peroxidase -
  AYJ58_RS09670 (AYJ58_09510) hemH 2176230..2177243 (+) 1014 WP_106649933.1 ferrochelatase -
  AYJ58_RS09675 (AYJ58_09515) ruvX 2177330..2177755 (-) 426 WP_006082513.1 Holliday junction resolvase RuvX -
  AYJ58_RS09680 (AYJ58_09520) - 2177785..2178348 (-) 564 WP_106649934.1 YqgE/AlgH family protein -
  AYJ58_RS09685 (AYJ58_09525) yciH 2178415..2178744 (-) 330 WP_106649935.1 stress response translation initiation inhibitor YciH -
  AYJ58_RS09690 (AYJ58_09530) - 2178923..2179240 (-) 318 WP_106649936.1 hypothetical protein -
  AYJ58_RS09695 (AYJ58_09535) - 2179420..2179980 (+) 561 WP_006082509.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40986.13 Da        Isoelectric Point: 6.2352

>NTDB_id=177393 AYJ58_RS09660 WP_106649931.1 2174358..2175470(+) (pilU) [Shewanella sp. Pdp11]
MDVLPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSSRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=177393 AYJ58_RS09660 WP_106649931.1 2174358..2175470(+) (pilU) [Shewanella sp. Pdp11]
ATGGATGTTCTTCCGTTTTTAAAAGTGATCGTTGAGCGCAAAGCCTCCGACTTATTTATCACTGCTGGTTTTCCACCGAG
TGCCAAGATTGACGGTGAATTGCGTCCTTTGGCTGAAAGTGCTTTTACTCCAGCCCAATCACTGGATTTTGTTGAATCCT
TAATGACCGAAGCGCAGAAACAAGAGTTTCATTCGAGTCGTGAGTGTAACTTTGCTTTTGCGGCAAAGGATTTAGGTCGT
TTTCGGGTGAGCGCCTTCTGGCAACGCGAATCGCCTGGCTGCGTGATGCGCCGTATTGAAACTAAAATCCCAGATGTCGA
TGATTTAAAACTGCCGCCTATTTTAAAAGATCTGGTGATGAGCAAACGGGGTCTTATCATTATGGTGGGCGGAACGGGGA
CGGGTAAGTCAACGTCCTTAGCGGCCTTGGTAGGCTATCGCAATGCCCATGCCCGTGGTCATATTTTAACTATCGAAGAT
CCCGTCGAATTTGTTCACGACCACAGAAAAAGTATTATCACCCAACGTGAAGTCGGCATAGATACAGAGTCTTTTGATGC
GGCGCTAAAAAGCTCCCTTCGCCAAGCCCCCGATGTGATCTTGATTGGTGAAATTCGCACCCAAGAAACGATGGAGTTCG
CGTTGTCATTTGCGGAAACGGGTCACCTTTGTATGGCAACCTTGCATGCCAACAATGCCAACCAAGCATTAGACCGGATT
ATGCATCTCGTGCCTGAGAGTAAACATCAACAATTGTTGTTCGATTTGTCATTGAATTTGCGCGGTATTGTGGCTCAGCA
ATTGATCCCAAAAGTCGATGGCACAGGGCGCCGCGCCGCGATTGAAGTCTTGATTAACACCCCAAGGGTGGCAAGTTTAA
TTGCGAAAAACGAGCTGCACTTGCTCAAAGAAACCATGGCCAAATCCAACGAGCAGGGCATGCAGACGTTCGATCAGGCC
TTGCTGAAACTTTATGTCGATGGCGAGATAAGTTACGCCGATGCGCTGCACCATGCTGATTCACCAAACGATTTACGTTT
GATGATTAAATTGCAGAATAAAGAATCCACGGGCTCAGGTTTTATGGAAGGTGTCACCTTAGACATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.946

100

0.603
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

60.795

95.135

0.578
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

97.297

0.395
  pilT Vibrio cholerae strain A1552

42.727

89.189

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.727

89.189

0.381
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362

Multiple sequence alignment