Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A4W93_RS28220 Genome accession   NZ_CP015118
Coordinates   6160483..6161526 (-) Length   347 a.a.
NCBI ID   WP_085753772.1    Uniprot ID   A0A1W6LGT2
Organism   Piscinibacter gummiphilus strain NS21     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6155483..6166526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W93_RS28190 (A4W93_28070) - 6155531..6155887 (+) 357 WP_085753766.1 hypothetical protein -
  A4W93_RS28195 (A4W93_28075) - 6155992..6157296 (+) 1305 WP_085753767.1 hemolysin family protein -
  A4W93_RS28200 (A4W93_28080) - 6157286..6157726 (+) 441 WP_085753768.1 hypothetical protein -
  A4W93_RS28205 (A4W93_28085) - 6157707..6158600 (-) 894 WP_085753769.1 NAD(P)-dependent oxidoreductase -
  A4W93_RS28210 (A4W93_28090) pilU 6158600..6159736 (-) 1137 WP_085753770.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A4W93_RS28215 (A4W93_28095) - 6159764..6160423 (-) 660 WP_085753771.1 cyclic nucleotide-binding domain-containing protein -
  A4W93_RS28220 (A4W93_28100) pilT 6160483..6161526 (-) 1044 WP_085753772.1 type IV pilus twitching motility protein PilT Machinery gene
  A4W93_RS28225 (A4W93_28105) - 6161600..6162301 (+) 702 WP_085753773.1 YggS family pyridoxal phosphate-dependent enzyme -
  A4W93_RS28230 (A4W93_28110) - 6162355..6163035 (+) 681 WP_099960009.1 helix-turn-helix transcriptional regulator -
  A4W93_RS28235 (A4W93_28115) - 6163032..6163571 (+) 540 WP_099960010.1 DUF6036 family nucleotidyltransferase -
  A4W93_RS28240 (A4W93_28120) - 6163568..6164437 (-) 870 WP_085753775.1 LysR family transcriptional regulator -
  A4W93_RS28245 (A4W93_28125) - 6164443..6165471 (-) 1029 WP_085753776.1 MBL fold metallo-hydrolase -
  A4W93_RS28250 (A4W93_28130) - 6165571..6165963 (-) 393 WP_085753777.1 tautomerase family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38135.78 Da        Isoelectric Point: 7.0742

>NTDB_id=176854 A4W93_RS28220 WP_085753772.1 6160483..6161526(-) (pilT) [Piscinibacter gummiphilus strain NS21]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLEHKQVHEMVYDIMNDSQRKAYEETLEVDFSFEIQGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLNAPKIFAELSLKPRGLVLVTGPTGSGKSTTLAGMVNHLNENEYGHVLTVED
PIEFVHESKKCLVNQREVGPHTLSFANALRSALREDPDAILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
VDVFPAAEKDMVRAMLSESLVAVISQTLCKLKDGTGRVAAHEIMLGTPAIRNLIRENKIAQMYSSIQTGNSVGMQTLDQN
LVDLVRRNIISPAEARGKAKSPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=176854 A4W93_RS28220 WP_085753772.1 6160483..6161526(-) (pilT) [Piscinibacter gummiphilus strain NS21]
ATGGACATCACCCAACTGCTGGCGTTTTCGGTCAAGAACAAGGCTTCCGACCTTCACCTCTCCGCCGGTCTGCCGCCGAT
GATCCGTGTGCACGGCGACGTGCGGCGCATCAACGTCGACCCCCTCGAGCACAAGCAGGTGCACGAGATGGTCTACGACA
TCATGAACGACTCGCAGCGCAAGGCCTACGAGGAAACGCTCGAAGTCGACTTCTCGTTCGAGATCCAGGGTCTCGCCCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGGACCATTCCGTCGAAGATCCTCACGCTCGA
GCAGCTCAACGCGCCGAAGATCTTCGCCGAGCTGTCGCTCAAGCCGCGCGGCCTCGTGCTGGTCACCGGCCCCACGGGTT
CGGGCAAGTCCACCACGCTCGCGGGCATGGTGAACCACCTCAACGAAAACGAGTACGGCCACGTGCTGACCGTGGAAGAC
CCGATCGAGTTCGTGCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAAGTCGGCCCGCACACGCTGAGCTTCGCCAA
CGCGCTGCGCTCCGCGCTTCGTGAAGACCCGGACGCCATCCTCGTGGGCGAAATGCGCGACCTGGAGACCATCCGCCTCG
CGCTGACCGCCGCCGAGACGGGCCACCTGGTGTTCGGCACGCTGCACACGTCCAGCGCCGCGAAGACCGTCGACCGCATC
GTCGACGTGTTCCCCGCGGCCGAGAAGGACATGGTGCGCGCGATGCTGTCGGAGTCGCTCGTCGCGGTGATCTCGCAGAC
GCTGTGCAAGCTCAAGGACGGCACCGGCCGCGTGGCCGCGCACGAGATCATGCTCGGCACGCCCGCGATCCGGAACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTATTCGTCCATCCAGACCGGCAACAGCGTGGGCATGCAGACGCTCGACCAGAAC
CTGGTCGACCTGGTGCGCCGCAACATCATCTCGCCCGCGGAAGCCCGCGGCAAGGCGAAGTCGCCGGAGAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W6LGT2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.101

99.424

0.767
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

72.566

97.695

0.709
  pilT Legionella pneumophila strain ERS1305867

72.566

97.695

0.709
  pilT Neisseria meningitidis 8013

68.696

99.424

0.683
  pilT Neisseria gonorrhoeae MS11

68.406

99.424

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.16

97.406

0.654
  pilT Vibrio cholerae strain A1552

67.16

97.406

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

41.159

99.424

0.409
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.58

99.424

0.403

Multiple sequence alignment