Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   A4W93_RS28210 Genome accession   NZ_CP015118
Coordinates   6158600..6159736 (-) Length   378 a.a.
NCBI ID   WP_085753770.1    Uniprot ID   -
Organism   Piscinibacter gummiphilus strain NS21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6153600..6164736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W93_RS28180 (A4W93_28060) - 6154273..6154869 (+) 597 WP_085754361.1 phosphoheptose isomerase -
  A4W93_RS28185 (A4W93_28065) - 6154866..6155531 (+) 666 WP_085753765.1 BON domain-containing protein -
  A4W93_RS28190 (A4W93_28070) - 6155531..6155887 (+) 357 WP_085753766.1 hypothetical protein -
  A4W93_RS28195 (A4W93_28075) - 6155992..6157296 (+) 1305 WP_085753767.1 hemolysin family protein -
  A4W93_RS28200 (A4W93_28080) - 6157286..6157726 (+) 441 WP_085753768.1 hypothetical protein -
  A4W93_RS28205 (A4W93_28085) - 6157707..6158600 (-) 894 WP_085753769.1 NAD(P)-dependent oxidoreductase -
  A4W93_RS28210 (A4W93_28090) pilU 6158600..6159736 (-) 1137 WP_085753770.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A4W93_RS28215 (A4W93_28095) - 6159764..6160423 (-) 660 WP_085753771.1 cyclic nucleotide-binding domain-containing protein -
  A4W93_RS28220 (A4W93_28100) pilT 6160483..6161526 (-) 1044 WP_085753772.1 type IV pilus twitching motility protein PilT Machinery gene
  A4W93_RS28225 (A4W93_28105) - 6161600..6162301 (+) 702 WP_085753773.1 YggS family pyridoxal phosphate-dependent enzyme -
  A4W93_RS28230 (A4W93_28110) - 6162355..6163035 (+) 681 WP_099960009.1 helix-turn-helix transcriptional regulator -
  A4W93_RS28235 (A4W93_28115) - 6163032..6163571 (+) 540 WP_099960010.1 DUF6036 family nucleotidyltransferase -
  A4W93_RS28240 (A4W93_28120) - 6163568..6164437 (-) 870 WP_085753775.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42197.47 Da        Isoelectric Point: 6.5924

>NTDB_id=176853 A4W93_RS28210 WP_085753770.1 6158600..6159736(-) (pilU) [Piscinibacter gummiphilus strain NS21]
MERDQASKFVNDLLRLMVSRNGSDLFLTADFPPAIKVDGKVTKVSPQPLTGQHTIALARAVMNDKQAAEFERTKECNFAI
SPQGIGRFRVNAFVQQSNIGLVMRTIPQTLPTIDALNLPQVLKDVTMTKRGLVIFVGATGSGKSTSLAAMVDFRNENSYG
HIITIEDPVEFVHPHKNCIVTQREVGLDTEGWEQALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKSLISQRLLPRQEGKGRIAAVEILLNTPLISDLIFKGEVSEIKEIMKKSRELG
MQTFDQALFDLYENQLVTYEDALRNADSVNDLRLQIKLHSNRARNADLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=176853 A4W93_RS28210 WP_085753770.1 6158600..6159736(-) (pilU) [Piscinibacter gummiphilus strain NS21]
ATGGAAAGAGACCAGGCATCGAAATTCGTCAACGACCTGCTGCGCCTGATGGTGTCGCGCAACGGGTCCGACCTCTTCCT
GACCGCCGACTTCCCGCCGGCCATCAAGGTGGACGGCAAGGTGACGAAGGTGTCGCCGCAACCCCTCACCGGGCAGCACA
CCATCGCGCTCGCGCGGGCGGTGATGAACGACAAGCAGGCCGCCGAGTTCGAGCGCACGAAGGAATGCAACTTCGCGATC
TCGCCGCAGGGCATCGGCCGCTTCCGGGTCAACGCCTTCGTGCAGCAGAGCAACATCGGCCTCGTGATGCGGACCATCCC
GCAGACCCTGCCCACCATCGACGCGCTGAACCTGCCGCAGGTGCTCAAGGACGTCACGATGACCAAGCGCGGCCTCGTGA
TCTTCGTGGGTGCCACCGGCTCCGGCAAGAGCACGTCGCTCGCCGCGATGGTGGACTTCCGCAACGAGAACTCGTATGGC
CACATCATCACCATCGAGGACCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCATCGTCACGCAGCGCGAGGTGGGCCT
CGACACCGAGGGCTGGGAGCAGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTCATCCTGATGGGTGAGATCCGCG
ACCGCGAGACGATGGAACACGCCGTGGCCTTCGCCGAGACGGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCCCTCGACCGCATCATCAACTTCTTCCCCGAGGAACGTCGCGCCCAGCTGCTGATGGACCTCTCGCTGAACCT
GAAGTCGCTGATCTCGCAGCGCCTGCTGCCGCGCCAGGAAGGCAAGGGCCGCATCGCCGCGGTCGAGATCCTGCTGAACA
CCCCCCTCATCTCCGACCTGATCTTCAAGGGCGAGGTCAGCGAGATCAAGGAGATCATGAAGAAGTCGCGCGAGCTGGGC
ATGCAGACCTTCGACCAGGCCCTGTTCGACCTGTACGAGAACCAGCTCGTCACGTACGAGGACGCGCTGCGCAACGCCGA
CTCCGTCAACGACCTGCGCCTGCAGATCAAGCTCCACAGCAACCGTGCCCGCAACGCCGACCTGGCCGCGGGCACCGAAC
ACCTGACCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.115

96.825

0.611
  pilU Acinetobacter baylyi ADP1

59.312

92.328

0.548
  pilU Vibrio cholerae strain A1552

53.994

96.032

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

42.478

89.683

0.381
  pilT Acinetobacter baumannii D1279779

43.243

88.095

0.381
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baumannii strain A118

43.243

88.095

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.533

83.862

0.365
  pilT Vibrio cholerae strain A1552

43.533

83.862

0.365
  pilT Legionella pneumophila strain ERS1305867

41.441

88.095

0.365
  pilT Legionella pneumophila strain Lp02

41.441

88.095

0.365

Multiple sequence alignment