Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A3Q34_RS04430 Genome accession   NZ_CP014944
Coordinates   1037051..1038094 (-) Length   347 a.a.
NCBI ID   WP_070374253.1    Uniprot ID   A0A1D8RST4
Organism   Colwellia sp. PAMC 20917     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1032051..1043094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3Q34_RS04405 (A3Q34_04405) rsmE 1032470..1033231 (+) 762 WP_070374248.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  A3Q34_RS04410 (A3Q34_04410) gshB 1033256..1034212 (+) 957 WP_070374249.1 glutathione synthase -
  A3Q34_RS04415 (A3Q34_04415) - 1034353..1034907 (+) 555 WP_070374250.1 YqgE/AlgH family protein -
  A3Q34_RS04420 (A3Q34_04420) ruvX 1034927..1035361 (+) 435 WP_070374251.1 Holliday junction resolvase RuvX -
  A3Q34_RS04425 (A3Q34_04425) pilU 1035481..1036608 (-) 1128 WP_070374252.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A3Q34_RS04430 (A3Q34_04430) pilT 1037051..1038094 (-) 1044 WP_070374253.1 type IV pilus twitching motility protein PilT Machinery gene
  A3Q34_RS04435 (A3Q34_04435) - 1038148..1038843 (+) 696 WP_331710945.1 YggS family pyridoxal phosphate-dependent enzyme -
  A3Q34_RS04440 (A3Q34_04440) proC 1038935..1039750 (+) 816 WP_070374255.1 pyrroline-5-carboxylate reductase -
  A3Q34_RS04445 (A3Q34_04445) - 1039793..1040341 (+) 549 WP_070374256.1 YggT family protein -
  A3Q34_RS04450 (A3Q34_04450) - 1040436..1040876 (+) 441 WP_070374257.1 DUF4426 domain-containing protein -
  A3Q34_RS04455 (A3Q34_04455) - 1040961..1041554 (+) 594 WP_070374258.1 XTP/dITP diphosphatase -
  A3Q34_RS04460 (A3Q34_04460) hemW 1041557..1042705 (+) 1149 WP_070374259.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38658.21 Da        Isoelectric Point: 5.4508

>NTDB_id=175244 A3Q34_RS04430 WP_070374253.1 1037051..1038094(-) (pilT) [Colwellia sp. PAMC 20917]
MDITELLAFSVENNASDLHLSAGTPPSIRVDGDVRKLNIPAFDEKDVNALVYDIMNDRQRKEYEENLEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSKILSLDDLGCPDIFREISDIPRGLVLVTGPTGSGKSTTLAAMVDYINNNKHDHILTIED
PIEFVHENKKCLINQREVHRDTLSFEAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKSMVRSMLSESLRAVISQTLIKKVGGGRVAAHEIMLGVPAIRNLIREDKIAQMYSAIQTGMSHGMQTMDQCL
QGLVNRGLITKQDAMEKAVDKNQFRGF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=175244 A3Q34_RS04430 WP_070374253.1 1037051..1038094(-) (pilT) [Colwellia sp. PAMC 20917]
ATGGATATTACCGAATTACTTGCCTTTAGTGTGGAAAATAATGCATCTGATCTACATTTATCTGCAGGCACTCCGCCTTC
AATTCGTGTTGACGGTGACGTACGTAAACTCAATATCCCAGCATTTGACGAGAAAGATGTTAATGCTCTGGTCTATGATA
TTATGAATGATCGTCAACGGAAAGAGTATGAAGAAAATTTAGAAGTCGATTTCTCTTTTGAAGTGCCAAATTTAGCACGT
TTTCGTGTCAATGCTTTTAATCAAAATCGTGGCCCTGCAGCAGTATTTCGTACTATTCCAAGCAAGATATTGTCCTTAGA
TGATTTAGGCTGTCCTGATATCTTTCGTGAAATTTCTGATATACCTCGAGGCTTAGTTTTAGTGACGGGTCCAACAGGCT
CAGGTAAATCAACCACTTTAGCGGCTATGGTCGATTATATTAATAACAACAAACACGATCATATTCTTACCATTGAAGAT
CCTATTGAGTTTGTTCACGAAAACAAAAAATGTTTAATAAACCAACGTGAGGTCCATCGCGATACACTAAGTTTTGAAGC
TGCTCTGCGCAGTGCTTTACGTGAAGACCCTGATGTTATTCTTGTTGGCGAAATGCGCGACTTAGAAACTATTCGTTTAG
CGATGACAGCGGCAGAAACCGGTCACTTAGTTTTTGGTACTTTACATACAACCTCTGCACCAAAAACCATCGACCGTATC
ATTGATGTTTTCCCCGGTGAAGAGAAATCTATGGTGCGTTCAATGTTGTCAGAATCACTTCGTGCGGTTATTTCACAAAC
CTTGATTAAAAAAGTGGGTGGGGGTCGTGTTGCCGCTCATGAGATAATGTTAGGTGTTCCTGCTATAAGAAATTTAATTC
GTGAAGACAAAATAGCGCAGATGTACAGTGCCATTCAAACTGGTATGTCTCATGGCATGCAAACCATGGATCAGTGCTTA
CAAGGTCTAGTGAATAGAGGCTTAATAACTAAGCAAGATGCGATGGAAAAAGCGGTTGATAAAAATCAATTTAGAGGTTT
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D8RST4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.135

0.775
  pilT Acinetobacter nosocomialis M2

77.616

99.135

0.769
  pilT Acinetobacter baumannii D1279779

77.616

99.135

0.769
  pilT Acinetobacter baumannii strain A118

77.616

99.135

0.769
  pilT Pseudomonas aeruginosa PAK

78.402

97.406

0.764
  pilT Acinetobacter baylyi ADP1

76.744

99.135

0.761
  pilT Legionella pneumophila strain Lp02

74.709

99.135

0.741
  pilT Legionella pneumophila strain ERS1305867

74.709

99.135

0.741
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.118

0.706
  pilT Vibrio cholerae strain A1552

72.7

97.118

0.706
  pilT Neisseria meningitidis 8013

68.732

97.695

0.671
  pilT Neisseria gonorrhoeae MS11

68.437

97.695

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.623

99.424

0.533
  pilU Vibrio cholerae strain A1552

42.197

99.712

0.421
  pilU Pseudomonas stutzeri DSM 10701

41.329

99.712

0.412
  pilU Acinetobacter baylyi ADP1

40.922

100

0.409

Multiple sequence alignment