Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   A3Q34_RS04425 Genome accession   NZ_CP014944
Coordinates   1035481..1036608 (-) Length   375 a.a.
NCBI ID   WP_070374252.1    Uniprot ID   A0A1D8RSU0
Organism   Colwellia sp. PAMC 20917     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1030481..1041608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3Q34_RS04400 (A3Q34_04400) - 1030948..1031928 (-) 981 WP_070377004.1 rhodanese-related sulfurtransferase -
  A3Q34_RS04405 (A3Q34_04405) rsmE 1032470..1033231 (+) 762 WP_070374248.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  A3Q34_RS04410 (A3Q34_04410) gshB 1033256..1034212 (+) 957 WP_070374249.1 glutathione synthase -
  A3Q34_RS04415 (A3Q34_04415) - 1034353..1034907 (+) 555 WP_070374250.1 YqgE/AlgH family protein -
  A3Q34_RS04420 (A3Q34_04420) ruvX 1034927..1035361 (+) 435 WP_070374251.1 Holliday junction resolvase RuvX -
  A3Q34_RS04425 (A3Q34_04425) pilU 1035481..1036608 (-) 1128 WP_070374252.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A3Q34_RS04430 (A3Q34_04430) pilT 1037051..1038094 (-) 1044 WP_070374253.1 type IV pilus twitching motility protein PilT Machinery gene
  A3Q34_RS04435 (A3Q34_04435) - 1038148..1038843 (+) 696 WP_331710945.1 YggS family pyridoxal phosphate-dependent enzyme -
  A3Q34_RS04440 (A3Q34_04440) proC 1038935..1039750 (+) 816 WP_070374255.1 pyrroline-5-carboxylate reductase -
  A3Q34_RS04445 (A3Q34_04445) - 1039793..1040341 (+) 549 WP_070374256.1 YggT family protein -
  A3Q34_RS04450 (A3Q34_04450) - 1040436..1040876 (+) 441 WP_070374257.1 DUF4426 domain-containing protein -
  A3Q34_RS04455 (A3Q34_04455) - 1040961..1041554 (+) 594 WP_070374258.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41144.15 Da        Isoelectric Point: 5.7366

>NTDB_id=175243 A3Q34_RS04425 WP_070374252.1 1035481..1036608(-) (pilU) [Colwellia sp. PAMC 20917]
MNFHDLLQKMVKEGASDMFVTAKLAVSAKVNGELQAIDDHLLTSDESLALVHNAMNDKQKKQFTEEKECNFAISIDDIGR
FRISAFWQRDMAGMVVRRIVTEIPDADELGLPPILKDVVMSKRGLVLFVGGTGTGKSTSMASLIGYRNKNSRGHILTIED
PVEFVHEHARCMVTQREVGLDTESFDAALKSSLRQAPDVILIGEIRNQETMEHALSFAETGHLCIATLHANNANQAIDRI
MHLVPADQHGKLLFDLALNLRGIIAQQLIPTCDGNGRVAAIEILLNSPYIAELIKKGDIGSIKEVMEKSKELGMQTFDGA
LFELYQKGSINYADALHHADSPNDLRLMIKLRSNEQGGTGALSGVTIDGFGPKDV

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=175243 A3Q34_RS04425 WP_070374252.1 1035481..1036608(-) (pilU) [Colwellia sp. PAMC 20917]
ATGAATTTTCATGACTTATTACAAAAGATGGTAAAAGAAGGTGCCTCTGATATGTTTGTTACCGCTAAGCTTGCGGTAAG
CGCCAAAGTAAATGGCGAGTTACAAGCAATCGATGATCACCTTTTAACAAGCGATGAATCGTTAGCGCTTGTTCATAACG
CAATGAACGATAAACAGAAAAAGCAATTTACTGAAGAAAAAGAATGTAACTTTGCCATTTCAATTGACGACATTGGCCGC
TTCCGTATTTCAGCCTTTTGGCAACGCGATATGGCAGGTATGGTTGTCCGACGTATCGTCACAGAAATTCCTGATGCTGA
CGAACTCGGTTTACCGCCAATCTTAAAAGATGTAGTCATGTCAAAGCGCGGTTTAGTACTTTTTGTTGGTGGCACGGGTA
CGGGTAAATCAACATCAATGGCTTCATTGATTGGCTATCGTAATAAAAATTCGCGTGGCCATATTCTTACCATTGAAGAC
CCTGTTGAATTTGTTCATGAACATGCGCGTTGTATGGTAACGCAACGAGAGGTTGGCTTAGACACTGAAAGTTTCGATGC
GGCCTTAAAAAGCTCATTACGCCAAGCACCCGATGTTATCTTAATTGGTGAGATCCGTAACCAAGAAACCATGGAACATG
CGTTAAGCTTTGCTGAAACCGGGCACTTATGTATCGCAACCTTGCATGCAAATAATGCTAACCAAGCGATTGACCGTATT
ATGCATTTAGTGCCTGCGGATCAACATGGCAAGTTATTGTTTGATTTAGCGTTAAATTTACGCGGTATTATAGCTCAGCA
ACTGATCCCTACCTGCGACGGTAACGGGCGTGTTGCGGCGATTGAGATCTTACTTAACTCGCCTTATATCGCTGAACTTA
TTAAAAAAGGTGATATTGGTAGTATTAAAGAAGTGATGGAAAAGTCTAAAGAACTTGGCATGCAGACCTTTGATGGTGCT
TTGTTTGAGTTGTATCAAAAAGGCTCAATAAACTATGCTGATGCATTGCATCATGCTGATTCACCTAATGATTTACGCTT
GATGATTAAGTTGCGCAGTAATGAGCAAGGAGGCACAGGTGCATTAAGTGGCGTGACCATTGACGGTTTTGGCCCTAAAG
ATGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D8RSU0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.649

98.667

0.579
  pilU Acinetobacter baylyi ADP1

59.091

93.867

0.555
  pilU Vibrio cholerae strain A1552

55.163

98.133

0.541
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.047

96.8

0.397
  pilT Pseudomonas aeruginosa PAK

41.159

92

0.379
  pilT Legionella pneumophila strain ERS1305867

41.39

88.267

0.365
  pilT Legionella pneumophila strain Lp02

41.39

88.267

0.365
  pilT Acinetobacter baylyi ADP1

40.058

91.2

0.365
  pilT Pseudomonas stutzeri DSM 10701

39.42

92

0.363
  pilT Acinetobacter nosocomialis M2

39.474

91.2

0.36
  pilT Acinetobacter baumannii D1279779

39.474

91.2

0.36
  pilT Acinetobacter baumannii strain A118

39.474

91.2

0.36

Multiple sequence alignment