Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A3Q33_RS02260 Genome accession   NZ_CP014943
Coordinates   556418..557461 (-) Length   347 a.a.
NCBI ID   WP_081149400.1    Uniprot ID   A0A222G503
Organism   Colwellia sp. PAMC 21821     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 551418..562461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3Q33_RS02235 (A3Q33_02225) - 552408..552962 (+) 555 WP_081178474.1 YqgE/AlgH family protein -
  A3Q33_RS02240 (A3Q33_02230) ruvX 552962..553399 (+) 438 WP_081149408.1 Holliday junction resolvase RuvX -
  A3Q33_RS02245 (A3Q33_02235) - 553493..554446 (+) 954 WP_081178476.1 AEC family transporter -
  A3Q33_RS02250 (A3Q33_02240) - 554443..554988 (-) 546 WP_231295768.1 DsrE family protein -
  A3Q33_RS02255 (A3Q33_02245) pilU 555264..556391 (-) 1128 WP_081178478.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A3Q33_RS02260 (A3Q33_02250) pilT 556418..557461 (-) 1044 WP_081149400.1 type IV pilus twitching motility protein PilT Machinery gene
  A3Q33_RS02265 (A3Q33_02255) - 557531..558205 (+) 675 WP_081178480.1 YggS family pyridoxal phosphate-dependent enzyme -
  A3Q33_RS02270 (A3Q33_02260) proC 558342..559154 (+) 813 WP_081178482.1 pyrroline-5-carboxylate reductase -
  A3Q33_RS02275 (A3Q33_02265) - 559259..559807 (+) 549 WP_081178484.1 YggT family protein -
  A3Q33_RS02280 (A3Q33_02270) - 559879..560319 (+) 441 WP_081178487.1 DUF4426 domain-containing protein -
  A3Q33_RS02285 (A3Q33_02275) - 560680..561279 (+) 600 WP_081178489.1 XTP/dITP diphosphatase -
  A3Q33_RS02290 (A3Q33_02280) hemW 561280..562425 (+) 1146 WP_081178492.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38642.11 Da        Isoelectric Point: 5.7804

>NTDB_id=175222 A3Q33_RS02260 WP_081149400.1 556418..557461(-) (pilT) [Colwellia sp. PAMC 21821]
MDITELLAFSVENNASDLHLSTGTPPSIRVDGDVRKLNIPAFDEKDVNALVYDIMNDRQRKEYEENLEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSKILSLDDLGCPDIFRDISELPRGLVLVTGPTGSGKSTTLAAMVDHINKTKHDHILTIED
PIEFVHQNHSCLINQREVHRDTLSFSAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKAMVRSMLSESLRAVISQTLVKKVGGGRVAAHEIMMGVPAIRNLIREDKIAQMYSVIQTGMSHGMQTMDQCL
QNLVSRGMITRQDAMDKAQDKNQFSGY

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=175222 A3Q33_RS02260 WP_081149400.1 556418..557461(-) (pilT) [Colwellia sp. PAMC 21821]
ATGGATATTACCGAATTACTCGCATTTAGTGTGGAAAATAATGCTTCAGATTTACATTTATCAACGGGTACACCCCCATC
TATTCGCGTCGATGGTGACGTACGTAAGCTAAATATACCAGCATTTGACGAAAAAGATGTCAACGCTTTGGTCTATGATA
TTATGAATGATCGCCAACGCAAAGAGTATGAAGAAAATTTAGAGGTCGATTTCTCTTTTGAAGTGCCAAATTTAGCGCGC
TTCAGGGTCAATGCTTTTAACCAAAACCGTGGTCCTGCCGCTGTATTTCGAACCATTCCTAGTAAAATATTGTCATTAGA
CGACTTAGGTTGTCCTGATATTTTTCGGGATATTTCGGAATTACCTCGTGGCTTAGTTTTGGTTACTGGTCCTACGGGTT
CAGGTAAATCCACAACGCTTGCGGCCATGGTTGATCATATTAACAAAACTAAACACGATCACATTTTAACCATTGAAGAC
CCTATCGAATTTGTTCATCAAAACCATTCATGTTTAATTAACCAACGTGAAGTACACCGTGACACATTAAGTTTTAGCGC
GGCATTGCGCTCAGCGTTACGTGAAGATCCCGATGTTATTTTAGTTGGTGAAATGCGTGACTTAGAAACCATACGTTTAG
CGATGACCGCCGCGGAAACTGGCCATTTAGTTTTTGGTACTTTGCATACAACTTCAGCGCCAAAAACTATTGACCGTATT
ATTGATGTATTCCCAGGTGAAGAAAAAGCCATGGTGCGTTCAATGCTTTCAGAATCATTACGTGCGGTAATTTCTCAAAC
ACTGGTTAAAAAAGTTGGTGGTGGTCGTGTAGCTGCGCACGAAATTATGATGGGTGTGCCGGCCATTCGTAACCTTATCC
GTGAAGATAAAATTGCGCAAATGTATTCTGTTATTCAAACCGGTATGTCTCATGGTATGCAAACTATGGATCAATGTTTA
CAAAACCTTGTTAGTCGGGGCATGATCACCAGACAAGATGCCATGGACAAAGCACAAGATAAAAACCAATTTAGTGGTTA
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A222G503

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.163

99.135

0.755
  pilT Pseudomonas stutzeri DSM 10701

76.163

99.135

0.755
  pilT Acinetobacter baumannii strain A118

75.872

99.135

0.752
  pilT Acinetobacter baumannii D1279779

75.872

99.135

0.752
  pilT Pseudomonas aeruginosa PAK

75

99.135

0.744
  pilT Acinetobacter baylyi ADP1

75

99.135

0.744
  pilT Legionella pneumophila strain Lp02

72.674

99.135

0.72
  pilT Legionella pneumophila strain ERS1305867

72.674

99.135

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.091

97.695

0.695
  pilT Vibrio cholerae strain A1552

71.091

97.695

0.695
  pilT Neisseria meningitidis 8013

67.919

99.712

0.677
  pilT Neisseria gonorrhoeae MS11

67.63

99.712

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.89

99.712

0.527
  pilU Pseudomonas stutzeri DSM 10701

41.399

98.847

0.409
  pilU Vibrio cholerae strain A1552

40.816

98.847

0.403
  pilU Acinetobacter baylyi ADP1

39.826

99.135

0.395

Multiple sequence alignment