Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   A3Q33_RS02255 Genome accession   NZ_CP014943
Coordinates   555264..556391 (-) Length   375 a.a.
NCBI ID   WP_081178478.1    Uniprot ID   -
Organism   Colwellia sp. PAMC 21821     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 550264..561391
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3Q33_RS02225 (A3Q33_02215) rsmE 550453..551199 (+) 747 WP_081178470.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  A3Q33_RS02230 (A3Q33_02220) gshB 551379..552332 (+) 954 WP_081178472.1 glutathione synthase -
  A3Q33_RS02235 (A3Q33_02225) - 552408..552962 (+) 555 WP_081178474.1 YqgE/AlgH family protein -
  A3Q33_RS02240 (A3Q33_02230) ruvX 552962..553399 (+) 438 WP_081149408.1 Holliday junction resolvase RuvX -
  A3Q33_RS02245 (A3Q33_02235) - 553493..554446 (+) 954 WP_081178476.1 AEC family transporter -
  A3Q33_RS02250 (A3Q33_02240) - 554443..554988 (-) 546 WP_231295768.1 DsrE family protein -
  A3Q33_RS02255 (A3Q33_02245) pilU 555264..556391 (-) 1128 WP_081178478.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A3Q33_RS02260 (A3Q33_02250) pilT 556418..557461 (-) 1044 WP_081149400.1 type IV pilus twitching motility protein PilT Machinery gene
  A3Q33_RS02265 (A3Q33_02255) - 557531..558205 (+) 675 WP_081178480.1 YggS family pyridoxal phosphate-dependent enzyme -
  A3Q33_RS02270 (A3Q33_02260) proC 558342..559154 (+) 813 WP_081178482.1 pyrroline-5-carboxylate reductase -
  A3Q33_RS02275 (A3Q33_02265) - 559259..559807 (+) 549 WP_081178484.1 YggT family protein -
  A3Q33_RS02280 (A3Q33_02270) - 559879..560319 (+) 441 WP_081178487.1 DUF4426 domain-containing protein -
  A3Q33_RS02285 (A3Q33_02275) - 560680..561279 (+) 600 WP_081178489.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41011.92 Da        Isoelectric Point: 5.5439

>NTDB_id=175221 A3Q33_RS02255 WP_081178478.1 555264..556391(-) (pilU) [Colwellia sp. PAMC 21821]
MNFHDLLQKMVHHEASDMFVTAKLPVSAKVNGELVPIDDQALSAAESLGLVHDAMSDKQKLEFDEEKECNFAISIDGIGR
FRVSAFWQRDMAGMVIRRIVTEIPEADDLGLPSVLKDVVMSKRGLVLFVGGTGTGKSTSMAALIGYRNKNSRGHILTIED
PVEFVHEHAKCMVTQREVGLDTESFGAALKSSLRQAPDVILIGEIRNQETMEHALSFAETGHLCIATLHANNANQAIDRI
MHLVPADQHGKLLFDLALNLRGIVAQQLIPTRDGNGRLAAIEILLNSPYIAELIKKGDIGSIKEVMEKSTDQGMQTFDQA
LFNLYQQGLINYADALHHADSPNDLRLMIKLRSNDQGGTGSLSGVTIDGLAPKEV

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=175221 A3Q33_RS02255 WP_081178478.1 555264..556391(-) (pilU) [Colwellia sp. PAMC 21821]
ATGAATTTTCATGATTTATTACAAAAAATGGTGCATCACGAAGCATCAGATATGTTTGTTACGGCTAAATTGCCGGTAAG
TGCAAAAGTTAATGGTGAGCTTGTTCCTATTGATGATCAGGCTTTATCTGCAGCCGAGTCTTTAGGTTTGGTTCATGATG
CGATGTCGGATAAACAAAAACTTGAATTTGACGAAGAGAAAGAATGTAACTTCGCAATTTCAATTGACGGTATTGGCCGA
TTTCGTGTTTCAGCATTTTGGCAACGTGATATGGCAGGCATGGTTATTCGTCGTATTGTGACCGAAATACCAGAAGCGGA
TGATCTTGGTTTACCTTCGGTACTCAAAGACGTGGTGATGTCAAAACGTGGCTTAGTCTTATTTGTTGGTGGCACGGGTA
CGGGTAAATCAACCTCGATGGCCGCGCTAATTGGCTATCGTAATAAAAATTCTCGCGGCCATATTTTGACCATAGAAGAT
CCTGTTGAATTTGTTCATGAACACGCTAAATGTATGGTAACGCAACGCGAAGTAGGGCTAGATACAGAAAGTTTTGGTGC
TGCGCTAAAAAGCTCTTTACGCCAAGCCCCTGACGTTATATTGATTGGTGAAATTCGAAACCAAGAAACGATGGAGCATG
CATTGAGTTTCGCAGAAACGGGTCACTTGTGTATCGCTACCTTGCATGCCAATAATGCAAACCAAGCTATTGATCGTATT
ATGCATTTAGTACCTGCAGACCAGCATGGTAAGCTGCTGTTTGATCTTGCGTTAAACCTACGTGGCATAGTTGCTCAACA
ATTAATTCCAACGCGTGATGGGAATGGTCGCTTAGCCGCGATTGAAATCTTACTAAACTCGCCTTATATCGCTGAGTTAA
TTAAAAAAGGTGATATTGGTAGCATTAAAGAAGTGATGGAAAAATCTACCGACCAAGGTATGCAAACATTTGACCAAGCA
CTTTTTAATTTATATCAACAAGGTCTGATCAATTACGCTGATGCCTTACATCATGCAGATTCACCAAATGATTTACGCTT
AATGATCAAACTGCGTAGTAATGATCAAGGTGGAACAGGCTCATTGTCTGGTGTCACTATTGATGGTTTAGCGCCGAAAG
AAGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.025

95.2

0.6
  pilU Vibrio cholerae strain A1552

56.793

98.133

0.557
  pilU Acinetobacter baylyi ADP1

58.807

93.867

0.552
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.785

99.2

0.395
  pilT Pseudomonas aeruginosa PAK

40.58

92

0.373
  pilT Legionella pneumophila strain ERS1305867

41.692

88.267

0.368
  pilT Legionella pneumophila strain Lp02

41.692

88.267

0.368
  pilT Acinetobacter baylyi ADP1

40.351

91.2

0.368
  pilT Pseudomonas stutzeri DSM 10701

39.71

92

0.365
  pilT Acinetobacter nosocomialis M2

40.058

91.2

0.365
  pilT Acinetobacter baumannii D1279779

40.058

91.2

0.365
  pilT Acinetobacter baumannii strain A118

40.058

91.2

0.365

Multiple sequence alignment