Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A3224_RS00890 Genome accession   NZ_CP014864
Coordinates   203269..204303 (-) Length   344 a.a.
NCBI ID   WP_067150239.1    Uniprot ID   A0A143HHX7
Organism   Microbulbifer thermotolerans strain DAU221     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 198269..209303
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3224_RS00870 (A3224_00870) argH 198519..199928 (-) 1410 WP_067150229.1 argininosuccinate lyase -
  A3224_RS00875 (A3224_00875) - 200019..201149 (+) 1131 WP_067150233.1 sensor histidine kinase -
  A3224_RS00880 (A3224_00880) - 201155..201886 (+) 732 WP_067157233.1 LytTR family DNA-binding domain-containing protein -
  A3224_RS00885 (A3224_00885) pilU 201973..203124 (-) 1152 WP_067150236.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A3224_RS00890 (A3224_00890) pilT 203269..204303 (-) 1035 WP_067150239.1 type IV pilus twitching motility protein PilT Machinery gene
  A3224_RS00895 (A3224_00895) - 204413..205108 (+) 696 WP_067150242.1 YggS family pyridoxal phosphate-dependent enzyme -
  A3224_RS00900 (A3224_00900) proC 205125..205952 (+) 828 WP_231902426.1 pyrroline-5-carboxylate reductase -
  A3224_RS00905 (A3224_00905) - 206092..206700 (+) 609 WP_067150245.1 YggT family protein -
  A3224_RS00910 (A3224_00910) - 207030..209000 (+) 1971 WP_067150248.1 dynamin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38357.00 Da        Isoelectric Point: 6.5769

>NTDB_id=174806 A3224_RS00890 WP_067150239.1 203269..204303(-) (pilT) [Microbulbifer thermotolerans strain DAU221]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHSLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMDDLGMGTVFRQICDTPRGLVLVTGPTGSGKSTTLAAMIDYINDNKYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQAVISQTLLKRNGGGRVAAHEIMRGTPAIRNLIREDKVAQMYSAIQTGANVGMQTMDQCL
QDLVGKRIISREAAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=174806 A3224_RS00890 WP_067150239.1 203269..204303(-) (pilT) [Microbulbifer thermotolerans strain DAU221]
ATGGACATTACAGAACTCCTCGCCTTCAGCGCCAAACAAAACGCCTCCGACCTGCACCTGTCCGCGGGACTGCCGCCCAT
GATCCGTGTGGACGGTGACGTGCGCCGCATCAACCTGCCGCCTATGGAGCACAAGCAGGTGCACTCTCTGATCTACGAGA
TCATGAACGACAAACAGCGCAAGGATTACGAGGAGTTCCTGGAGACCGACTTCTCCTTCGAGGTGCCCGGTGTGGCGCGC
TTCCGTGTCAACGCCTTCAACCACAACCGCGGTGCCGGCGCGGTTTTTCGGACCATTCCGTCCAAGGTCCTGACCATGGA
TGACCTGGGGATGGGCACGGTATTCCGTCAGATCTGCGACACGCCCCGCGGCCTGGTTCTGGTAACCGGCCCCACCGGCT
CCGGTAAGTCCACCACCCTGGCGGCGATGATCGACTACATCAACGACAACAAATACGAACACATCCTCACCATTGAGGAC
CCGATCGAATTTGTGCACGAATCCAAAAAGTGTCTGGTCAACCAGCGCGAGGTGCACCGGGACACCCACGGTTTCGCCGA
AGCCCTGCGCTCGGCCCTGCGGGAGGACCCGGACATTATTCTGGTGGGTGAGATGCGGGACCTGGAAACCATCCGCCTGG
CGCTCACCGCCGCCGAAACCGGCCACTTGGTATTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGCGTC
GTGGACGTTTTCCCGGCGGAAGAAAAGGCCATGGTGCGCTCCATGCTGTCGGAATCCCTGCAGGCGGTCATCTCGCAAAC
ACTGCTGAAAAGAAACGGCGGCGGCCGCGTGGCCGCCCACGAAATCATGCGCGGCACGCCGGCGATCCGGAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCCGCCATTCAAACCGGTGCCAACGTCGGCATGCAGACCATGGACCAGTGCCTC
CAAGACCTGGTGGGCAAGCGCATCATCAGTCGAGAGGCCGCGCGCGAGAAGGCCAAAATGCCGGAAAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A143HHX7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.953

100

0.89
  pilT Pseudomonas stutzeri DSM 10701

88.372

100

0.884
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

81.105

100

0.811
  pilT Legionella pneumophila strain Lp02

75.148

98.256

0.738
  pilT Legionella pneumophila strain ERS1305867

75.148

98.256

0.738
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.68
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.235

98.837

0.674
  pilT Vibrio cholerae strain A1552

68.235

98.837

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

40.118

98.547

0.395
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39

Multiple sequence alignment