Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   A3224_RS00885 Genome accession   NZ_CP014864
Coordinates   201973..203124 (-) Length   383 a.a.
NCBI ID   WP_067150236.1    Uniprot ID   A0A143HHY2
Organism   Microbulbifer thermotolerans strain DAU221     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 196973..208124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3224_RS17590 (A3224_00860) lptM 197871..198083 (-) 213 WP_067150223.1 LPS translocon maturation chaperone LptM -
  A3224_RS00865 (A3224_00865) cyaY 198173..198502 (+) 330 WP_067150226.1 iron donor protein CyaY -
  A3224_RS00870 (A3224_00870) argH 198519..199928 (-) 1410 WP_067150229.1 argininosuccinate lyase -
  A3224_RS00875 (A3224_00875) - 200019..201149 (+) 1131 WP_067150233.1 sensor histidine kinase -
  A3224_RS00880 (A3224_00880) - 201155..201886 (+) 732 WP_067157233.1 LytTR family DNA-binding domain-containing protein -
  A3224_RS00885 (A3224_00885) pilU 201973..203124 (-) 1152 WP_067150236.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A3224_RS00890 (A3224_00890) pilT 203269..204303 (-) 1035 WP_067150239.1 type IV pilus twitching motility protein PilT Machinery gene
  A3224_RS00895 (A3224_00895) - 204413..205108 (+) 696 WP_067150242.1 YggS family pyridoxal phosphate-dependent enzyme -
  A3224_RS00900 (A3224_00900) proC 205125..205952 (+) 828 WP_231902426.1 pyrroline-5-carboxylate reductase -
  A3224_RS00905 (A3224_00905) - 206092..206700 (+) 609 WP_067150245.1 YggT family protein -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42481.79 Da        Isoelectric Point: 6.3724

>NTDB_id=174805 A3224_RS00885 WP_067150236.1 201973..203124(-) (pilU) [Microbulbifer thermotolerans strain DAU221]
MDIERLLQLVVEKGASDLFVTAGVPPSIKVHGRVVPASSTSLTPEKARELVVGIMNAKQRKEFADKKELNFAIAVRNVGR
FRVSAFFQRNLVGMVLRRIETKIPTIDDLGLPDVLKDLAMTKRGLIIFVGATGTGKSTSLASMIGHRNENAKGHIITIED
PIEFIHPHRGCIVTQREVGLDTESFEVALKNTLRQAPDVILIGEVRSRETMEHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHQQLWMDLSLNLKAMVAQQLVPTPDGDGRRACLEIMINTPLMADLIRKGEVHKMKELMSRSNEQGMQTFDQA
LYELYDRGEITYEDALAHADSANDLRLMIKLKSESDAEYLNSAAGGLSLQNDSEYGDGGPQLY

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=174805 A3224_RS00885 WP_067150236.1 201973..203124(-) (pilU) [Microbulbifer thermotolerans strain DAU221]
ATGGATATTGAAAGACTTTTGCAACTGGTTGTAGAAAAAGGGGCTTCCGACCTCTTCGTCACCGCCGGTGTGCCTCCTTC
CATCAAAGTTCACGGCCGGGTAGTGCCCGCCAGCAGCACTTCGCTGACACCGGAAAAGGCGCGGGAGCTGGTGGTCGGCA
TTATGAATGCCAAACAGCGCAAGGAGTTCGCAGACAAAAAAGAACTCAACTTCGCCATCGCCGTGCGCAATGTAGGCCGT
TTCCGGGTCTCGGCCTTCTTCCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGTATCGAGACAAAAATCCCCACCATCGA
TGACCTGGGTCTGCCGGATGTGCTCAAGGATCTGGCGATGACCAAGCGCGGCCTGATTATTTTCGTCGGCGCCACCGGTA
CTGGTAAATCCACCTCCCTGGCCTCGATGATCGGCCACCGCAATGAAAACGCCAAAGGGCACATCATCACCATCGAAGAC
CCGATCGAATTCATCCACCCGCACCGCGGCTGTATCGTTACGCAGCGCGAAGTGGGCCTGGATACCGAGTCTTTCGAGGT
GGCTCTGAAAAACACCCTGCGGCAGGCACCCGACGTCATACTGATCGGTGAGGTCCGCTCCCGTGAAACCATGGAACACG
CCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTCTGCACTCTGCACGCCAACAACGCCAACCAGGCCCTGGACCGCATC
ATTCACTTCTTCCCGGCGGACCGCCACCAACAATTGTGGATGGACCTGTCGCTCAACCTGAAAGCCATGGTGGCACAACA
ACTGGTGCCGACTCCCGACGGCGATGGCCGCCGCGCTTGTCTCGAGATCATGATCAATACGCCACTGATGGCAGATCTGA
TCCGCAAAGGCGAAGTGCACAAAATGAAAGAGCTGATGAGCCGCTCCAATGAGCAGGGTATGCAGACCTTTGACCAGGCT
CTGTACGAGCTCTACGACCGCGGCGAAATCACCTACGAAGACGCCCTCGCCCACGCCGATTCCGCCAACGATCTGCGCCT
GATGATCAAACTCAAATCCGAATCGGACGCCGAGTACTTAAACAGCGCGGCGGGAGGGTTGAGCCTGCAAAACGATTCCG
AATACGGAGATGGCGGGCCGCAATTGTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A143HHY2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

75.135

96.606

0.726
  pilU Acinetobacter baylyi ADP1

66.947

93.211

0.624
  pilU Vibrio cholerae strain A1552

56.065

96.867

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.428

88.512

0.402
  pilT Acinetobacter nosocomialis M2

43.881

87.467

0.384
  pilT Acinetobacter baumannii D1279779

43.881

87.467

0.384
  pilT Acinetobacter baumannii strain A118

43.881

87.467

0.384
  pilT Pseudomonas aeruginosa PAK

42.687

87.467

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.515

87.206

0.371
  pilT Vibrio cholerae strain A1552

42.515

87.206

0.371
  pilT Legionella pneumophila strain ERS1305867

42.388

87.467

0.371
  pilT Legionella pneumophila strain Lp02

42.388

87.467

0.371
  pilT Acinetobacter baylyi ADP1

41.642

89.034

0.371
  pilT Pseudomonas stutzeri DSM 10701

42.09

87.467

0.368

Multiple sequence alignment