Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PG2T_RS08330 Genome accession   NZ_CP014671
Coordinates   1755888..1756925 (+) Length   345 a.a.
NCBI ID   WP_068804148.1    Uniprot ID   A0A1B1YTM6
Organism   Immundisolibacter cernigliae strain TR3.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1750888..1761925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG2T_RS08305 (PG2T_08320) yrfG 1752676..1753332 (-) 657 WP_068804138.1 GMP/IMP nucleotidase -
  PG2T_RS08310 (PG2T_08325) - 1753329..1753634 (-) 306 WP_068804140.1 DUF167 domain-containing protein -
  PG2T_RS08315 (PG2T_08330) - 1753631..1754224 (-) 594 WP_068804142.1 YggT family protein -
  PG2T_RS08320 (PG2T_08335) proC 1754228..1755049 (-) 822 WP_068804144.1 pyrroline-5-carboxylate reductase -
  PG2T_RS08325 (PG2T_08340) - 1755075..1755815 (-) 741 WP_418268535.1 YggS family pyridoxal phosphate-dependent enzyme -
  PG2T_RS08330 (PG2T_08345) pilT 1755888..1756925 (+) 1038 WP_068804148.1 type IV pilus twitching motility protein PilT Machinery gene
  PG2T_RS08335 (PG2T_08350) pilU 1756973..1758169 (+) 1197 WP_083215031.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PG2T_RS08340 (PG2T_08355) - 1758340..1759302 (-) 963 WP_068804152.1 NAD(P)H-dependent flavin oxidoreductase -
  PG2T_RS08345 (PG2T_08360) - 1759311..1759805 (-) 495 WP_068804154.1 aromatic-ring-hydroxylating dioxygenase subunit beta -
  PG2T_RS08350 (PG2T_08365) - 1759802..1761163 (-) 1362 WP_068804156.1 aromatic ring-hydroxylating oxygenase subunit alpha -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38491.15 Da        Isoelectric Point: 6.5062

>NTDB_id=173192 PG2T_RS08330 WP_068804148.1 1755888..1756925(+) (pilT) [Immundisolibacter cernigliae strain TR3.2]
MDITELLAFTVTNKASDLHLSAGMPPMIRVDGDVRRLDLPALDHSQVHDMVYDIMNDRQRKDYEEFLETDFSFEIPGLAR
FRVNAFNQHRGAAAAFRNVPNKILNLEELEAPQAFAELAMLPRGIVLVTGPTGSGKSTTLAAMLDEKNRAEYGHVVTIED
PIEFVHESKRCLINQREVHRDTLGFSEALRSALREDPDTILVGEMRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRI
IDVFPSGEKDMVRAMLSESLRAVIAQTLLKRIGGGRIAAHEIMMGTPAIRNLIRENKVAQIYSAIQTGQAHGMQTLDQCL
QKMVTHRKVSREDAAVRAVNKELFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=173192 PG2T_RS08330 WP_068804148.1 1755888..1756925(+) (pilT) [Immundisolibacter cernigliae strain TR3.2]
ATGGACATCACTGAACTGCTGGCATTCACGGTTACCAACAAGGCGTCCGATCTGCATCTGTCGGCGGGCATGCCGCCGAT
GATCCGCGTCGATGGCGATGTGCGCCGGCTCGACCTGCCGGCGCTGGACCACTCGCAGGTCCACGACATGGTGTACGACA
TCATGAACGACCGCCAGCGCAAGGACTACGAGGAGTTTCTGGAAACCGACTTCTCGTTCGAGATCCCCGGCCTGGCGCGT
TTTCGGGTCAACGCCTTCAACCAGCACCGCGGCGCGGCGGCGGCCTTCCGCAACGTGCCCAACAAGATCCTGAACCTGGA
GGAGCTCGAAGCACCGCAGGCCTTTGCCGAACTGGCCATGCTGCCGCGCGGCATCGTGCTGGTGACAGGACCCACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCCATGCTCGACGAGAAGAACCGGGCCGAATACGGCCATGTGGTCACCATCGAGGAT
CCGATCGAGTTCGTGCACGAGAGCAAGCGCTGCCTGATCAACCAGCGCGAGGTGCACCGCGATACGCTGGGCTTCAGCGA
GGCCCTGCGTTCGGCCCTGCGTGAGGACCCCGACACCATCCTGGTCGGCGAAATGCGCGACCTGGAAACGATCCGCCTGG
CGCTGACTGCGGCCGAAACCGGGCATCTGGTGTTCGCCACCCTGCACACCAGCTCGGCCGCCAAGACCATCGACCGCATC
ATCGACGTGTTTCCGTCGGGCGAGAAGGACATGGTGCGCGCCATGCTGTCCGAATCGCTGCGCGCGGTGATCGCGCAGAC
CCTGCTCAAGCGCATCGGCGGCGGACGCATCGCCGCGCACGAGATCATGATGGGCACGCCAGCGATTCGAAACCTGATCC
GCGAGAACAAGGTGGCGCAGATTTACTCCGCCATCCAGACCGGCCAGGCGCACGGCATGCAGACCCTGGACCAGTGTCTG
CAAAAGATGGTCACACATCGCAAAGTGAGCCGGGAAGACGCCGCCGTGCGTGCCGTCAACAAGGAACTGTTCCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B1YTM6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

75

99.71

0.748
  pilT Acinetobacter baumannii strain A118

75

99.71

0.748
  pilT Acinetobacter nosocomialis M2

75

99.71

0.748
  pilT Pseudomonas aeruginosa PAK

74.709

99.71

0.745
  pilT Pseudomonas stutzeri DSM 10701

73.837

99.71

0.736
  pilT Acinetobacter baylyi ADP1

73.547

99.71

0.733
  pilT Legionella pneumophila strain Lp02

70.64

99.71

0.704
  pilT Legionella pneumophila strain ERS1305867

70.64

99.71

0.704
  pilT Neisseria meningitidis 8013

65.598

99.42

0.652
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.444

93.913

0.652
  pilT Vibrio cholerae strain A1552

69.444

93.913

0.652
  pilT Neisseria gonorrhoeae MS11

65.306

99.42

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.116

100

0.481
  pilU Vibrio cholerae strain A1552

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

39.412

98.551

0.388
  pilU Pseudomonas stutzeri DSM 10701

38.938

98.261

0.383

Multiple sequence alignment