Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PG2T_RS08335 Genome accession   NZ_CP014671
Coordinates   1756973..1758169 (+) Length   398 a.a.
NCBI ID   WP_083215031.1    Uniprot ID   -
Organism   Immundisolibacter cernigliae strain TR3.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1751973..1763169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG2T_RS08305 (PG2T_08320) yrfG 1752676..1753332 (-) 657 WP_068804138.1 GMP/IMP nucleotidase -
  PG2T_RS08310 (PG2T_08325) - 1753329..1753634 (-) 306 WP_068804140.1 DUF167 domain-containing protein -
  PG2T_RS08315 (PG2T_08330) - 1753631..1754224 (-) 594 WP_068804142.1 YggT family protein -
  PG2T_RS08320 (PG2T_08335) proC 1754228..1755049 (-) 822 WP_068804144.1 pyrroline-5-carboxylate reductase -
  PG2T_RS08325 (PG2T_08340) - 1755075..1755815 (-) 741 WP_418268535.1 YggS family pyridoxal phosphate-dependent enzyme -
  PG2T_RS08330 (PG2T_08345) pilT 1755888..1756925 (+) 1038 WP_068804148.1 type IV pilus twitching motility protein PilT Machinery gene
  PG2T_RS08335 (PG2T_08350) pilU 1756973..1758169 (+) 1197 WP_083215031.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PG2T_RS08340 (PG2T_08355) - 1758340..1759302 (-) 963 WP_068804152.1 NAD(P)H-dependent flavin oxidoreductase -
  PG2T_RS08345 (PG2T_08360) - 1759311..1759805 (-) 495 WP_068804154.1 aromatic-ring-hydroxylating dioxygenase subunit beta -
  PG2T_RS08350 (PG2T_08365) - 1759802..1761163 (-) 1362 WP_068804156.1 aromatic ring-hydroxylating oxygenase subunit alpha -
  PG2T_RS08355 (PG2T_08370) - 1761377..1762174 (+) 798 WP_068804159.1 class II aldolase/adducin family protein -
  PG2T_RS08360 (PG2T_08375) - 1762204..1762599 (+) 396 WP_068804160.1 DUF4345 family protein -
  PG2T_RS08365 (PG2T_08380) - 1762665..1763045 (+) 381 WP_158513170.1 nuclear transport factor 2 family protein -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 43953.47 Da        Isoelectric Point: 6.4314

>NTDB_id=173193 PG2T_RS08335 WP_083215031.1 1756973..1758169(+) (pilU) [Immundisolibacter cernigliae strain TR3.2]
MDFATLLQMMVDQNSSDLFVTAGVAPSIKTHGAVKPACEGKFNAEQAREMCYGIMNERQRTQLENTFECQYAISARGIGR
FRVSTFIQRGNVGMVVRKIETHIPTLEELQVPMVLREISMIKRGLALMVGGTGTGKSTTLAAMVGYRNHNSTGHIITIED
PIEYLHDHAGCIVTQREVGVDTMSFEDALKNTLRQAPDVILIGEIRARETMEHAIAFAETGHLCLATLHANNANQALDRI
INFFPEDRRQQLLMDLSLNLRAIVSQRLLPTADGSGRRAAVEVMLASPLITDLIRKGLVHEIKPIMARSNELGMRTFDQA
LHALYAEGVITYDEAIANADSANDLRLLIKLERDGAATQMAQDSQKFSLKQEVKDEPAPLVFSRPLPMASRPGEGQNS

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=173193 PG2T_RS08335 WP_083215031.1 1756973..1758169(+) (pilU) [Immundisolibacter cernigliae strain TR3.2]
ATCGATTTCGCCACCCTGCTGCAGATGATGGTCGACCAGAACTCGTCGGACCTGTTCGTGACCGCCGGCGTCGCGCCCAG
CATCAAGACGCACGGCGCCGTGAAGCCGGCCTGTGAGGGAAAGTTCAACGCCGAACAGGCGCGCGAGATGTGCTACGGCA
TCATGAACGAGCGCCAGCGCACGCAGCTGGAGAACACCTTCGAGTGCCAGTACGCCATCTCGGCGCGCGGCATCGGGCGC
TTCCGCGTCAGCACCTTCATCCAGCGCGGCAACGTCGGCATGGTGGTGCGCAAGATCGAGACCCACATCCCGACCCTGGA
AGAGCTGCAGGTGCCGATGGTGCTGCGCGAGATCTCCATGATCAAGCGAGGCCTGGCGCTGATGGTGGGCGGCACCGGCA
CCGGCAAGTCGACCACCCTGGCGGCCATGGTCGGCTACCGCAACCACAACAGCACCGGCCACATCATCACCATCGAGGAT
CCGATCGAGTACCTGCACGACCACGCCGGCTGCATCGTCACCCAGCGCGAGGTGGGAGTGGACACCATGTCCTTCGAGGA
CGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCCGTGCCCGCGAAACCATGGAACACG
CCATCGCCTTTGCCGAAACCGGCCATCTGTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTTCCCGAGGACCGGCGCCAGCAGCTGCTGATGGACCTGTCGCTGAACCTGCGAGCGATCGTCTCGCAGCG
CCTGCTGCCGACTGCGGACGGCAGCGGCAGGCGAGCGGCGGTGGAGGTAATGCTGGCCAGCCCGCTGATCACGGACCTGA
TCCGCAAGGGCCTGGTGCACGAGATCAAGCCGATCATGGCGCGCTCGAACGAGCTTGGCATGCGCACCTTCGATCAGGCC
TTGCACGCCCTGTATGCCGAAGGCGTCATCACCTACGACGAGGCCATCGCCAACGCCGATTCGGCCAACGATTTGCGCCT
GCTGATCAAGCTGGAGCGCGATGGCGCGGCGACCCAGATGGCGCAGGATTCGCAGAAATTCTCACTCAAGCAGGAGGTCA
AGGACGAGCCGGCACCGCTGGTGTTCAGCCGTCCGCTGCCGATGGCCTCCCGACCGGGCGAAGGCCAGAACAGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.698

94.975

0.595
  pilU Acinetobacter baylyi ADP1

57.865

89.447

0.518
  pilU Vibrio cholerae strain A1552

52.975

88.693

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

89.698

0.377

Multiple sequence alignment