Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AX767_RS08505 Genome accession   NZ_CP014517
Coordinates   1705373..1706416 (+) Length   347 a.a.
NCBI ID   WP_068630398.1    Uniprot ID   A0A126ZB91
Organism   Variovorax sp. PAMC 28711 strain PAMC28711     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1700373..1711416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AX767_RS08475 (AX767_08475) - 1700532..1701782 (+) 1251 WP_068630390.1 CoA transferase -
  AX767_RS08480 (AX767_08480) - 1701779..1702231 (+) 453 WP_068630392.1 PaaI family thioesterase -
  AX767_RS08485 (AX767_08485) - 1702228..1703388 (+) 1161 WP_068630394.1 CoA transferase -
  AX767_RS08490 (AX767_08490) - 1703424..1704089 (+) 666 WP_068633488.1 XRE family transcriptional regulator -
  AX767_RS08495 (AX767_08495) - 1704086..1704634 (+) 549 WP_068630395.1 GNAT family N-acetyltransferase -
  AX767_RS08500 (AX767_08500) - 1704597..1705325 (-) 729 WP_068630396.1 YggS family pyridoxal phosphate-dependent enzyme -
  AX767_RS08505 (AX767_08505) pilT 1705373..1706416 (+) 1044 WP_068630398.1 type IV pilus twitching motility protein PilT Machinery gene
  AX767_RS08510 (AX767_08510) pilU 1706430..1707566 (+) 1137 WP_068630400.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AX767_RS08515 (AX767_08515) - 1707601..1708524 (+) 924 WP_068630402.1 NAD(P)-dependent oxidoreductase -
  AX767_RS08520 (AX767_08520) - 1708538..1709305 (-) 768 WP_068630405.1 BON domain-containing protein -
  AX767_RS08525 (AX767_08525) - 1709302..1709922 (-) 621 WP_068630408.1 SIS domain-containing protein -
  AX767_RS08530 (AX767_08530) - 1709977..1710384 (-) 408 WP_068630410.1 YraN family protein -
  AX767_RS08535 (AX767_08535) rsmI 1710384..1711328 (+) 945 WP_068630413.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38075.80 Da        Isoelectric Point: 6.8884

>NTDB_id=172027 AX767_RS08505 WP_068630398.1 1705373..1706416(+) (pilT) [Variovorax sp. PAMC 28711 strain PAMC28711]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIEGLAR
FRVNAFNQSRGAAAVFRTIPSKILSLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKDMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREGKVAQMYSSIQTGQGQGMQTLDQN
LTDLVRRSVISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=172027 AX767_RS08505 WP_068630398.1 1705373..1706416(+) (pilT) [Variovorax sp. PAMC 28711 strain PAMC28711]
GTGGACATTACCCAATTGCTGGCGTTCAGCGTCAAGAACAAGGCTTCGGACTTGCACCTTTCGGCGGGTCTGCCGCCGAT
GATCCGGGTCCACGGGGACGTGCGGCGCATCAACGTCGATGCGCTCGACCACAAGGCGGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTTCTGGAAGTCGACTTTTCGTTCGAGATCGAAGGCCTGGCGCGC
TTCCGGGTGAACGCGTTCAACCAGTCGCGCGGCGCGGCGGCGGTGTTCCGGACGATTCCCTCGAAGATCCTGTCGCTCGA
GCAGCTCAACGCGCCCAAGATCTTCGGCGACCTGGCGCTCAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCAACTACCTCAACGAAACCGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTCCACGAATCCAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGGCCGATGACGCTGAGCTTCGCGGC
GGCGTTGCGCTCCGCGCTGCGCGAAGACCCGGACGCGATCCTGGTTGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCATGACCGCGGCCGAAACCGGCCATCTGGTGTTCGGCACCCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAAGAGAAAGACATGATCCGCGCGATGCTGTCGGAATCGCTGCAGGCGGTGATTTCACAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTCGGCACGCCGGCCATCCGGAATTTGA
TCCGCGAAGGCAAGGTTGCGCAAATGTATTCGTCGATCCAGACTGGCCAGGGCCAGGGCATGCAGACGCTCGACCAGAAC
CTGACCGACCTGGTACGCCGCAGCGTGATCAGCGCGGCCGAGGCACGCGGCAAGGCCAAGATTCCCGAAAACTTCCCCGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A126ZB91

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.328

98.271

0.671
  pilT Vibrio cholerae strain A1552

68.328

98.271

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

43.284

96.542

0.418
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.542

0.392

Multiple sequence alignment