Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AX767_RS08510 Genome accession   NZ_CP014517
Coordinates   1706430..1707566 (+) Length   378 a.a.
NCBI ID   WP_068630400.1    Uniprot ID   -
Organism   Variovorax sp. PAMC 28711 strain PAMC28711     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1701430..1712566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AX767_RS08480 (AX767_08480) - 1701779..1702231 (+) 453 WP_068630392.1 PaaI family thioesterase -
  AX767_RS08485 (AX767_08485) - 1702228..1703388 (+) 1161 WP_068630394.1 CoA transferase -
  AX767_RS08490 (AX767_08490) - 1703424..1704089 (+) 666 WP_068633488.1 XRE family transcriptional regulator -
  AX767_RS08495 (AX767_08495) - 1704086..1704634 (+) 549 WP_068630395.1 GNAT family N-acetyltransferase -
  AX767_RS08500 (AX767_08500) - 1704597..1705325 (-) 729 WP_068630396.1 YggS family pyridoxal phosphate-dependent enzyme -
  AX767_RS08505 (AX767_08505) pilT 1705373..1706416 (+) 1044 WP_068630398.1 type IV pilus twitching motility protein PilT Machinery gene
  AX767_RS08510 (AX767_08510) pilU 1706430..1707566 (+) 1137 WP_068630400.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AX767_RS08515 (AX767_08515) - 1707601..1708524 (+) 924 WP_068630402.1 NAD(P)-dependent oxidoreductase -
  AX767_RS08520 (AX767_08520) - 1708538..1709305 (-) 768 WP_068630405.1 BON domain-containing protein -
  AX767_RS08525 (AX767_08525) - 1709302..1709922 (-) 621 WP_068630408.1 SIS domain-containing protein -
  AX767_RS08530 (AX767_08530) - 1709977..1710384 (-) 408 WP_068630410.1 YraN family protein -
  AX767_RS08535 (AX767_08535) rsmI 1710384..1711328 (+) 945 WP_068630413.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  AX767_RS08540 (AX767_08540) - 1711338..1712066 (-) 729 WP_068630415.1 glutathione S-transferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41852.01 Da        Isoelectric Point: 6.5064

>NTDB_id=172028 AX767_RS08510 WP_068630400.1 1706430..1707566(+) (pilU) [Variovorax sp. PAMC 28711 strain PAMC28711]
MERDQASQFINELLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSGQALGAQHTLALTRSVMNDRQTAEFERTKECNFAI
SPTGVGRFRVNAFVQQGKVGMVMRTIPAKLPTIDDLGMPQVLKEVAMTKRGLTIMVGATGSGKSTTLAAMIDWRNANSYG
HIVTVEDPVEFVHPHKNCVITQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGQGRIAAVEILLNTPLISDMIFKGEVGEIKEIMRKSRNLG
MQTFDQALFDLFENNAISFEEAIRNADSANDLRLQIKLNSRRAKSADLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=172028 AX767_RS08510 WP_068630400.1 1706430..1707566(+) (pilU) [Variovorax sp. PAMC 28711 strain PAMC28711]
ATGGAACGCGATCAGGCCAGCCAGTTCATCAACGAATTGCTCAAGCTCATGGTGAGCCGCAACGGGAGCGACTTGTTCAT
CACCGCCGACTTTCCGCCCGCCATCAAGGTCGACGGCAAGGTGACCAAGGTGTCGGGGCAGGCACTGGGCGCGCAGCACA
CGCTGGCGCTCACGCGCTCGGTCATGAACGACCGCCAGACCGCCGAATTCGAGCGCACCAAGGAGTGCAACTTCGCGATT
TCCCCGACCGGCGTCGGCCGCTTTCGCGTGAACGCGTTCGTGCAGCAGGGCAAGGTCGGCATGGTGATGCGGACGATCCC
GGCGAAACTGCCCACCATCGACGATCTGGGCATGCCGCAGGTGCTCAAGGAAGTGGCCATGACCAAGCGCGGCCTGACCA
TCATGGTCGGCGCGACGGGCTCGGGCAAATCGACCACGCTGGCCGCGATGATCGACTGGCGCAATGCGAACTCCTACGGT
CACATCGTGACGGTCGAAGACCCGGTCGAATTCGTGCATCCGCACAAGAACTGCGTGATCACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCTGCACTAAAAAACACGTTGCGTCAGGCGCCCGACGTGATCCTGATGGGTGAAATCCGCG
ATCGCGAGACCATGGAACACGCCGTGGCCTTCGCCGAGACCGGCCACCTCTGCATGGCGACGCTGCACGCGAACAGCGCC
AATCAGGCGCTCGACCGGATCATCAACTTCTTCCCCGAAGAGCGTCGCGCGCAATTGCTGATGGACCTGTCGCTCAACCT
GCGCTCGCTGGTGTCGCAACGCCTCGTGCCGACCGAGGACGGCCAGGGTCGTATCGCCGCGGTCGAGATTTTGCTGAACA
CGCCGCTGATCTCCGACATGATCTTCAAGGGCGAGGTCGGCGAGATCAAGGAGATCATGCGAAAGAGCCGGAACCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGAACAACGCGATCAGTTTCGAAGAAGCGATTCGCAACGCCGA
CTCGGCCAACGACCTGCGCCTGCAGATCAAGCTGAACAGCCGGCGCGCGAAATCCGCCGATCTGTCCGCCGGCACCGAGC
ACTTCGCGATTGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.751

92.328

0.579
  pilU Acinetobacter baylyi ADP1

58.38

94.709

0.553
  pilU Vibrio cholerae strain A1552

54.023

92.063

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

44.807

89.153

0.399
  pilT Legionella pneumophila strain Lp02

44.681

87.037

0.389
  pilT Legionella pneumophila strain ERS1305867

44.681

87.037

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.47

87.831

0.373
  pilT Vibrio cholerae strain A1552

42.47

87.831

0.373
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373

Multiple sequence alignment