Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PcP3B5_RS02650 Genome accession   NZ_CP014158
Coordinates   584870..585904 (+) Length   344 a.a.
NCBI ID   WP_009615237.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain P3B5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 579870..590904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PcP3B5_RS02620 (PcP3B5_05310) - 579899..580090 (-) 192 WP_009615231.1 hypothetical protein -
  PcP3B5_RS02625 (PcP3B5_05320) - 580153..582120 (-) 1968 WP_061560514.1 dynamin-like GTPase family protein -
  PcP3B5_RS02630 (PcP3B5_05330) - 582232..582528 (-) 297 WP_058487695.1 DUF167 domain-containing protein -
  PcP3B5_RS02635 (PcP3B5_05340) - 582668..583261 (-) 594 WP_009615234.1 YggT family protein -
  PcP3B5_RS02640 (PcP3B5_05350) proC 583272..584090 (-) 819 WP_061560515.1 pyrroline-5-carboxylate reductase -
  PcP3B5_RS02645 (PcP3B5_05360) - 584107..584796 (-) 690 WP_061563194.1 YggS family pyridoxal phosphate-dependent enzyme -
  PcP3B5_RS02650 (PcP3B5_05370) pilT 584870..585904 (+) 1035 WP_009615237.1 type IV pilus twitching motility protein PilT Machinery gene
  PcP3B5_RS02655 (PcP3B5_05380) pilU 586009..587154 (+) 1146 WP_009615238.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PcP3B5_RS02660 (PcP3B5_05390) - 587448..587852 (+) 405 WP_043269499.1 NINE protein -
  PcP3B5_RS02665 (PcP3B5_05400) - 587996..589369 (+) 1374 WP_061560516.1 pyridoxal-phosphate dependent enzyme -
  PcP3B5_RS02670 (PcP3B5_05410) - 589366..590538 (+) 1173 WP_043269495.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37852.73 Da        Isoelectric Point: 6.7752

>NTDB_id=168914 PcP3B5_RS02650 WP_009615237.1 584870..585904(+) (pilT) [Pseudomonas citronellolis strain P3B5]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFKRVSDVPRGLVLVTGPTGSGKSTSLAAMLDYLNNTKYSHILTVED
PIEFVHESKKCLVNQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAEEKAMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDMCL
KGLVAKGLVTRDAAKEKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=168914 PcP3B5_RS02650 WP_009615237.1 584870..585904(+) (pilT) [Pseudomonas citronellolis strain P3B5]
ATGGATATTACCGAGTTGCTCGCCTTCAGCGCCAAGCAAGGCGCTTCGGACCTGCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGCCGCATCAACCTGCCGCCGCTGGAGCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAATTCCTGGAAACCGACTTCTCCTTCGAAGTGCCGGGCGTGGCGCGT
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTGCTGACCATGGA
GGATCTGGGCATGGGCGAAGTGTTCAAGCGGGTTTCCGACGTGCCGCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGTT
CGGGCAAGTCCACCTCGCTCGCGGCGATGCTCGACTACCTGAACAACACCAAGTACAGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCTCGGCTTCAACGA
GGCGCTGCGCTCGGCCCTGCGTGAGGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCCGCCGAGGAGAAGGCCATGGTCCGTTCCATGCTCTCCGAATCGCTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCGGCGCGCTGGGCATGCAGACCCTCGACATGTGCCTC
AAGGGCCTGGTCGCCAAGGGCCTGGTGACCCGCGACGCGGCGAAGGAAAAGGCGAAGATTCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

95.93

100

0.959
  pilT Pseudomonas stutzeri DSM 10701

93.314

100

0.933
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

75.74

98.256

0.744
  pilT Legionella pneumophila strain ERS1305867

75.74

98.256

0.744
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.529

98.837

0.677
  pilT Vibrio cholerae strain A1552

68.529

98.837

0.677
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395

Multiple sequence alignment