Detailed information NTG0168915

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PcP3B5_RS02655
• Genome accession: NZ_CP014158
• Coordinates: 586009..587154 (+)
• Length: 381 a.a.
• NCBI ID: WP_009615238.1
• Uniprot ID: A0A1A5D0E6
• Organism: Pseudomonas citronellolis strain P3B5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 581009..592154

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PcP3B5_RS02630 (PcP3B5_05330)	-	582232..582528 (-)	297	WP_058487695.1	DUF167 domain-containing protein	-
PcP3B5_RS02635 (PcP3B5_05340)	-	582668..583261 (-)	594	WP_009615234.1	YggT family protein	-
PcP3B5_RS02640 (PcP3B5_05350)	proC	583272..584090 (-)	819	WP_061560515.1	pyrroline-5-carboxylate reductase	-
PcP3B5_RS02645 (PcP3B5_05360)	-	584107..584796 (-)	690	WP_061563194.1	YggS family pyridoxal phosphate-dependent enzyme	-
PcP3B5_RS02650 (PcP3B5_05370)	pilT	584870..585904 (+)	1035	WP_009615237.1	type IV pilus twitching motility protein PilT	Machinery gene
PcP3B5_RS02655 (PcP3B5_05380)	pilU	586009..587154 (+)	1146	WP_009615238.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PcP3B5_RS02660 (PcP3B5_05390)	-	587448..587852 (+)	405	WP_043269499.1	NINE protein	-
PcP3B5_RS02665 (PcP3B5_05400)	-	587996..589369 (+)	1374	WP_061560516.1	pyridoxal-phosphate dependent enzyme	-
PcP3B5_RS02670 (PcP3B5_05410)	-	589366..590538 (+)	1173	WP_043269495.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42685.06 Da
• Isoelectric Point: 6.7792

>NTDB_id=168915 PcP3B5_RS02655 WP_009615238.1 586009..587154(+) (pilU) [Pseudomonas citronellolis strain P3B5]
MEFEKLLRLMVEKGASDLFVTAGVPPSMKVNGKVLPVTKTPLSPEQTRETVLAVMNEQQRRDFAEHHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTIEDLKLPEILKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDTFEVALKNTLRQAPDVIMIGEVRSRETMEHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHQQIWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYSQGEITYEDALAYADSANDLRLMIKLGSETDADHLSSMTQGLTLELSEDDPNRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=168915 PcP3B5_RS02655 WP_009615238.1 586009..587154(+) (pilU) [Pseudomonas citronellolis strain P3B5]
ATGGAATTCGAGAAGCTTCTGCGGCTGATGGTGGAGAAGGGCGCCTCGGACCTCTTCGTCACCGCCGGCGTGCCCCCGTC
GATGAAGGTCAACGGCAAGGTGCTGCCGGTGACCAAGACCCCGCTGTCGCCCGAGCAGACCCGCGAGACGGTGCTGGCGG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGCACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGGCGC
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGTTGCGCCGCATCGAGACCAACATCCCGACCATCGA
GGACCTCAAGCTGCCGGAGATCCTCAAGAAACTGGCCATGACCAAGCGCGGGCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGATACCGACACCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGCGAGACCATGGAGCACG
CCGTGGCCTTCGCCGAGACCGGCCACTTGTGTCTGGCCACCCTGCATGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGGCACCAGCAGATCTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGCTGAATACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGTGAGGTGCATGAGCTGAAGCCGTTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGATCAGGCG
CTGTACCAGCTCTATTCCCAGGGTGAGATCACCTATGAGGATGCGCTGGCCTATGCCGACTCGGCCAACGATCTGCGGTT
GATGATCAAGCTTGGGTCGGAGACCGATGCGGATCATCTGTCGAGCATGACTCAGGGGTTGACCCTGGAGCTGAGCGAAG
ACGATCCGAATCGCCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-270)

3D structure

• AlphaFold DB: A0A1A5D0E6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	87.664	100	0.877
pilU	Acinetobacter baylyi ADP1	63.032	98.688	0.622
pilU	Vibrio cholerae strain A1552	60.571	91.864	0.556
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.363	88.976	0.386