Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AUP74_RS05490 Genome accession   NZ_CP014143
Coordinates   1273385..1274419 (-) Length   344 a.a.
NCBI ID   WP_069946696.1    Uniprot ID   A0A1C9W5Y9
Organism   Microbulbifer aggregans strain CCB-MM1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1268385..1279419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AUP74_RS05470 (AUP74_01108) argH 1268647..1270080 (-) 1434 WP_069946693.1 argininosuccinate lyase -
  AUP74_RS05475 (AUP74_01109) - 1270225..1271361 (+) 1137 WP_083260840.1 sensor histidine kinase -
  AUP74_RS05480 (AUP74_01110) - 1271358..1272104 (+) 747 WP_069946694.1 LytR/AlgR family response regulator transcription factor -
  AUP74_RS05485 (AUP74_01111) pilU 1272123..1273271 (-) 1149 WP_069946695.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AUP74_RS05490 (AUP74_01112) pilT 1273385..1274419 (-) 1035 WP_069946696.1 type IV pilus twitching motility protein PilT Machinery gene
  AUP74_RS05495 (AUP74_01113) - 1274541..1275236 (+) 696 WP_069946697.1 YggS family pyridoxal phosphate-dependent enzyme -
  AUP74_RS05500 (AUP74_01114) - 1275273..1277318 (-) 2046 WP_069946698.1 alpha/beta hydrolase family protein -
  AUP74_RS05505 (AUP74_01115) proC 1277523..1278335 (+) 813 WP_069946699.1 pyrroline-5-carboxylate reductase -
  AUP74_RS05510 (AUP74_01116) - 1278419..1279027 (+) 609 WP_069946700.1 YggT family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38413.02 Da        Isoelectric Point: 6.4332

>NTDB_id=168690 AUP74_RS05490 WP_069946696.1 1273385..1274419(-) (pilT) [Microbulbifer aggregans strain CCB-MM1]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHALIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMDDLGMGQVFRQICDTPRGLVLVTGPTGSGKSTSLAAMIDYINDNKYEHILTVED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQAVVSQTLLKRNGGGRVAAHEIMRGTPAIRNLIREDKVAQMYSAIQTGASVGMQTMDQCL
QDLVERRVITRDVAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=168690 AUP74_RS05490 WP_069946696.1 1273385..1274419(-) (pilT) [Microbulbifer aggregans strain CCB-MM1]
ATGGACATCACAGAACTCCTCGCCTTCAGTGCCAAGCAGAACGCCTCGGACCTCCACCTGTCCGCGGGGCTGCCGCCAAT
GATCCGTGTCGATGGCGATGTCCGCCGCATTAACCTGCCGCCGATGGAGCACAAGCAGGTACACGCGCTGATCTACGAGA
TCATGAATGACAAGCAGCGCAAGGATTACGAGGAATTCCTGGAAACCGACTTCTCCTTCGAAGTGCCCGGCGTCGCCCGC
TTCCGTGTAAATGCGTTTAACCACAACCGCGGTGCCGGTGCCGTGTTCCGGACCATTCCGTCCAAGGTACTGACCATGGA
CGATCTGGGGATGGGCCAGGTATTCCGCCAGATCTGTGACACCCCCCGCGGCCTGGTGCTGGTGACCGGGCCCACCGGCT
CCGGTAAGTCCACCAGCCTGGCGGCAATGATCGACTACATTAACGACAACAAGTACGAGCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACGAATCGAAAAAGTGCCTGGTGAACCAGCGGGAAGTACACCGGGACACCCACGGCTTTGCCGA
GGCCCTGCGTTCCGCCCTGCGTGAGGACCCGGACATCATCCTCGTTGGTGAGATGCGGGACCTGGAGACCATCCGCCTGG
CCCTGACTGCGGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGGGTG
GTGGATGTATTCCCGGCGGAAGAAAAGGCAATGGTGCGCTCGATGCTGTCAGAATCCCTGCAGGCGGTGGTTTCCCAGAC
CCTGCTGAAGCGCAATGGCGGCGGCCGGGTGGCGGCCCATGAGATCATGCGTGGCACCCCAGCAATCCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCTGCCATCCAGACCGGTGCCAGCGTGGGCATGCAGACCATGGACCAGTGCCTG
CAGGATCTGGTGGAGCGCCGGGTGATCACCCGCGATGTGGCCCGTGAGAAGGCGAAGATGCCCGAAAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C9W5Y9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

80.233

100

0.802
  pilT Legionella pneumophila strain Lp02

74.556

98.256

0.733
  pilT Legionella pneumophila strain ERS1305867

74.556

98.256

0.733
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.909

95.64

0.669
  pilT Vibrio cholerae strain A1552

69.909

95.64

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

38.15

100

0.384
  pilU Vibrio cholerae strain A1552

40.615

94.477

0.384

Multiple sequence alignment