Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AUP74_RS05485 Genome accession   NZ_CP014143
Coordinates   1272123..1273271 (-) Length   382 a.a.
NCBI ID   WP_069946695.1    Uniprot ID   A0A1C9W5Z1
Organism   Microbulbifer aggregans strain CCB-MM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1267123..1278271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AUP74_RS05460 (AUP74_01106) lptM 1267945..1268205 (-) 261 WP_069946691.1 LPS translocon maturation chaperone LptM -
  AUP74_RS05465 (AUP74_01107) cyaY 1268292..1268618 (+) 327 WP_069946692.1 iron donor protein CyaY -
  AUP74_RS05470 (AUP74_01108) argH 1268647..1270080 (-) 1434 WP_069946693.1 argininosuccinate lyase -
  AUP74_RS05475 (AUP74_01109) - 1270225..1271361 (+) 1137 WP_083260840.1 sensor histidine kinase -
  AUP74_RS05480 (AUP74_01110) - 1271358..1272104 (+) 747 WP_069946694.1 LytR/AlgR family response regulator transcription factor -
  AUP74_RS05485 (AUP74_01111) pilU 1272123..1273271 (-) 1149 WP_069946695.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AUP74_RS05490 (AUP74_01112) pilT 1273385..1274419 (-) 1035 WP_069946696.1 type IV pilus twitching motility protein PilT Machinery gene
  AUP74_RS05495 (AUP74_01113) - 1274541..1275236 (+) 696 WP_069946697.1 YggS family pyridoxal phosphate-dependent enzyme -
  AUP74_RS05500 (AUP74_01114) - 1275273..1277318 (-) 2046 WP_069946698.1 alpha/beta hydrolase family protein -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42561.87 Da        Isoelectric Point: 6.2401

>NTDB_id=168689 AUP74_RS05485 WP_069946695.1 1272123..1273271(-) (pilU) [Microbulbifer aggregans strain CCB-MM1]
MEIERLLQLVVEKGASDLFITAGVPPSIKVHGKVIPASSSALTPEKARELVVGVMNEKQRREFAEKKELNFAIAVRNVGR
FRVSAFFQRNLVGMVLRRIETRIPTVDGLGLPEILKDLAMTKRGLIIFVGATGTGKSTSLASMIGHRNENSKGHIISIED
PIEFVHQHRGCIVTQREVGLDTESFEVALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHQQLYMDLSLNLKAMVAQQLVPTPDGDGRRACLEIMINTPLMADLIRKGEVHKMKELMKRSNEQGMQTFDQA
LYELYDRGEITYEDALSHADSANDLRLMIKLKSESDAEYLNDAAGELSLEGDSDEYGGPKLY

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=168689 AUP74_RS05485 WP_069946695.1 1272123..1273271(-) (pilU) [Microbulbifer aggregans strain CCB-MM1]
ATGGAAATCGAAAGACTGTTACAGCTGGTTGTGGAGAAAGGGGCATCGGACCTGTTCATCACCGCCGGTGTACCGCCCTC
GATCAAGGTTCACGGTAAGGTCATCCCGGCCAGCAGCTCAGCGCTGACGCCAGAGAAGGCCCGCGAGCTGGTGGTGGGTG
TGATGAACGAGAAGCAGCGTCGCGAATTCGCCGAAAAGAAAGAGCTGAACTTCGCTATTGCCGTGCGCAATGTGGGCCGC
TTCCGGGTTTCCGCCTTCTTCCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCCGTATCCCCACGGTCGA
CGGTCTGGGACTGCCGGAGATCCTCAAGGATCTGGCGATGACCAAACGCGGCCTGATCATCTTCGTGGGTGCGACCGGTA
CCGGTAAGTCCACCTCACTGGCATCCATGATCGGCCACCGCAACGAAAACTCGAAAGGTCACATTATCTCCATCGAGGAC
CCGATCGAATTCGTCCACCAGCACCGCGGGTGTATTGTCACCCAGCGCGAGGTGGGCCTGGATACCGAATCTTTCGAGGT
CGCCCTGAAGAACACCCTGCGTCAGGCGCCCGACGTGATTCTCATTGGTGAGGTGCGCTCGCGCGAGACCATGGATCACG
CCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGTGCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGGATC
ATCCACTTCTTCCCGGCGGACCGTCACCAGCAGCTCTATATGGACCTGTCGCTGAACCTCAAGGCGATGGTCGCCCAGCA
GCTGGTACCCACACCGGATGGCGACGGCCGCCGCGCCTGTCTCGAGATCATGATCAACACCCCGCTGATGGCGGACCTGA
TCCGCAAGGGCGAGGTGCACAAGATGAAGGAGTTGATGAAACGCTCCAACGAGCAGGGCATGCAGACCTTTGACCAGGCC
CTGTACGAGCTGTACGACCGCGGCGAGATCACCTACGAGGACGCCCTCTCCCACGCCGACTCCGCTAACGACCTGCGCCT
GATGATCAAGCTCAAGTCGGAATCCGATGCCGAGTACCTGAACGATGCAGCGGGCGAGCTGAGCCTCGAGGGCGACAGCG
ACGAATACGGCGGACCGAAGCTGTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C9W5Z1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

75.332

98.691

0.743
  pilU Acinetobacter baylyi ADP1

66.387

93.455

0.62
  pilU Vibrio cholerae strain A1552

57.865

93.194

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.133

88.743

0.401
  pilT Acinetobacter nosocomialis M2

43.953

88.743

0.39
  pilT Acinetobacter baumannii D1279779

43.953

88.743

0.39
  pilT Acinetobacter baumannii strain A118

43.953

88.743

0.39
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.027

88.22

0.38
  pilT Vibrio cholerae strain A1552

43.027

88.22

0.38
  pilT Acinetobacter baylyi ADP1

41.935

89.267

0.374
  pilT Legionella pneumophila strain Lp02

42.388

87.696

0.372
  pilT Legionella pneumophila strain ERS1305867

42.388

87.696

0.372
  pilT Pseudomonas aeruginosa PAK

41.593

88.743

0.369
  pilT Pseudomonas stutzeri DSM 10701

41.194

87.696

0.361

Multiple sequence alignment