Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AL543_RS12295 Genome accession   NZ_CP014043
Coordinates   1139624..1140730 (+) Length   368 a.a.
NCBI ID   WP_061052040.1    Uniprot ID   -
Organism   Vibrio mimicus strain FDAARGOS_112     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1134624..1145730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL543_RS12260 (AL543_11520) - 1134858..1135460 (-) 603 WP_061052036.1 XTP/dITP diphosphatase -
  AL543_RS12265 (AL543_11525) - 1135530..1135961 (-) 432 WP_061052037.1 DUF4426 domain-containing protein -
  AL543_RS12270 (AL543_11530) yggU 1136055..1136348 (-) 294 WP_000741913.1 DUF167 family protein YggU -
  AL543_RS12275 (AL543_11535) - 1136348..1136905 (-) 558 WP_005509546.1 YggT family protein -
  AL543_RS12280 (AL543_11540) proC 1136963..1137781 (-) 819 WP_061052038.1 pyrroline-5-carboxylate reductase -
  AL543_RS12285 (AL543_11545) - 1137836..1138546 (-) 711 WP_061052039.1 YggS family pyridoxal phosphate-dependent enzyme -
  AL543_RS12290 (AL543_11550) pilT 1138572..1139609 (+) 1038 WP_000350205.1 type IV pilus twitching motility protein PilT Machinery gene
  AL543_RS12295 (AL543_11555) pilU 1139624..1140730 (+) 1107 WP_061052040.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AL543_RS12300 (AL543_11560) - 1140818..1141615 (+) 798 WP_061052041.1 helix-turn-helix transcriptional regulator -
  AL543_RS12305 (AL543_11565) tyrS 1141725..1143005 (+) 1281 WP_061052042.1 tyrosine--tRNA ligase -
  AL543_RS12310 (AL543_11570) ruvX 1143030..1143452 (-) 423 WP_061052043.1 Holliday junction resolvase RuvX -
  AL543_RS12315 (AL543_11575) - 1143533..1144096 (-) 564 WP_001054773.1 YqgE/AlgH family protein -
  AL543_RS12320 (AL543_11580) gshB 1144132..1145088 (-) 957 WP_061052044.1 glutathione synthase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41306.38 Da        Isoelectric Point: 7.3350

>NTDB_id=167884 AL543_RS12295 WP_061052040.1 1139624..1140730(+) (pilU) [Vibrio mimicus strain FDAARGOS_112]
MELNQYLDGMLSHKASDLYITVGAPILYRVDGELRAQGEVLSLANVTTLLHAMMDDARQAEFKQTREANFAVVRESGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPAVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMQYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIGQQLLRDKNGKGRHGVFEVLLNSPRVADLIRRGDLHELKTTMARSKEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDNYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=167884 AL543_RS12295 WP_061052040.1 1139624..1140730(+) (pilU) [Vibrio mimicus strain FDAARGOS_112]
ATGGAGTTGAATCAATATCTGGATGGGATGCTGAGCCATAAAGCATCGGATCTTTACATCACCGTTGGAGCGCCGATTTT
ATATCGGGTCGATGGTGAATTGCGCGCGCAAGGTGAAGTGCTCAGTTTGGCGAATGTGACGACCTTACTGCATGCGATGA
TGGATGACGCGAGACAAGCGGAATTTAAGCAGACGCGTGAAGCGAACTTTGCTGTAGTACGCGAAAGTGGACGTTTCCGG
GTTAGTGCGTTTTTCCAACGTGAATTGCCGGGAGCGGTGATCCGCCGGATTGAAACGCGTATTCCCACCTTTGAAGAGCT
GAAATTACCCGCGGTACTACAAAATTTAGCGATCGCGAAACGCGGTTTAGTCTTAGTAGTGGGTGCTACGGGGTCAGGTA
AATCGACCACTATGGCGGCAATGACGGGGTATCGTAACCAGCACCGTACTGGACATATTTTGACGGTGGAGGATCCGATT
GAGTTTGTGCATGAACATAAGCGCTGTATCGTCACCCAACGTGAAGTTGGCTTAGATACAGAAAGCTATGAAGTGGCGTT
GAAAAACTCGCTGCGCCAAGCACCGGATATGATTTTGATTGGTGAAATTCGCAGCCGAGAAACCATGCAATACGCGATGA
CCTTTGCGGAAACGGGGCATTTGTGTATGGCCACGCTGCATGCCAACAACGCCAACCAAGCTTTAGAGCGGATTTTGCAT
TTAGTGCCGAAAGAACAGCGTGAGCAGTTCTTGCTCGACTTGTCACTCAACCTAAAAGGGGTGATTGGCCAGCAACTGTT
GCGCGATAAAAACGGCAAAGGGCGACATGGGGTGTTTGAAGTTCTGCTCAATAGTCCAAGAGTCGCGGATTTGATTCGCC
GCGGCGATCTGCATGAGCTTAAAACGACGATGGCGCGTTCCAAAGAAGTAGGCATGCAGACCTTCGACCAAGCCTTGTAC
CAACTGGTGGTGGACGACAAAATCAGCGAGCAAGATGCCCTGCACAGTGCGGATTCGGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATAACTACGGCAGCGGTTCCTTGCAGAATGTGAAGATTGATATGGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

96.196

100

0.962
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.484

84.239

0.383
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

40.708

92.12

0.375
  pilT Acinetobacter baumannii D1279779

40.708

92.12

0.375
  pilT Acinetobacter baumannii strain A118

40.708

92.12

0.375
  pilT Pseudomonas aeruginosa PAK

40.413

92.12

0.372
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37
  pilT Acinetobacter baylyi ADP1

41.846

88.315

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.823

92.12

0.367
  pilT Neisseria gonorrhoeae MS11

38.329

94.293

0.361
  pilT Neisseria meningitidis 8013

38.551

93.75

0.361

Multiple sequence alignment