Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AL543_RS12290 Genome accession   NZ_CP014043
Coordinates   1138572..1139609 (+) Length   345 a.a.
NCBI ID   WP_000350205.1    Uniprot ID   D2YE46
Organism   Vibrio mimicus strain FDAARGOS_112     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1133572..1144609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL543_RS12255 (AL543_11515) hemW 1133689..1134864 (-) 1176 WP_061052035.1 radical SAM family heme chaperone HemW -
  AL543_RS12260 (AL543_11520) - 1134858..1135460 (-) 603 WP_061052036.1 XTP/dITP diphosphatase -
  AL543_RS12265 (AL543_11525) - 1135530..1135961 (-) 432 WP_061052037.1 DUF4426 domain-containing protein -
  AL543_RS12270 (AL543_11530) yggU 1136055..1136348 (-) 294 WP_000741913.1 DUF167 family protein YggU -
  AL543_RS12275 (AL543_11535) - 1136348..1136905 (-) 558 WP_005509546.1 YggT family protein -
  AL543_RS12280 (AL543_11540) proC 1136963..1137781 (-) 819 WP_061052038.1 pyrroline-5-carboxylate reductase -
  AL543_RS12285 (AL543_11545) - 1137836..1138546 (-) 711 WP_061052039.1 YggS family pyridoxal phosphate-dependent enzyme -
  AL543_RS12290 (AL543_11550) pilT 1138572..1139609 (+) 1038 WP_000350205.1 type IV pilus twitching motility protein PilT Machinery gene
  AL543_RS12295 (AL543_11555) pilU 1139624..1140730 (+) 1107 WP_061052040.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AL543_RS12300 (AL543_11560) - 1140818..1141615 (+) 798 WP_061052041.1 helix-turn-helix transcriptional regulator -
  AL543_RS12305 (AL543_11565) tyrS 1141725..1143005 (+) 1281 WP_061052042.1 tyrosine--tRNA ligase -
  AL543_RS12310 (AL543_11570) ruvX 1143030..1143452 (-) 423 WP_061052043.1 Holliday junction resolvase RuvX -
  AL543_RS12315 (AL543_11575) - 1143533..1144096 (-) 564 WP_001054773.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38248.81 Da        Isoelectric Point: 6.5572

>NTDB_id=167883 AL543_RS12290 WP_000350205.1 1138572..1139609(+) (pilT) [Vibrio mimicus strain FDAARGOS_112]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLVFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQSRGCSAVFRTIPTVIPTLDQLEAPEIFTKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=167883 AL543_RS12290 WP_000350205.1 1138572..1139609(+) (pilT) [Vibrio mimicus strain FDAARGOS_112]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGTGAAGTTAGGAAGCTCGGCGTGCCTGCTTTTACTCATTCTGACGTACATCGCTTGGTATTTGAAA
TAATGAATGATGCCCAGCGCAGTGAATATGAAGAAAAATTAGAAGTCGATTTTTCTTTTGAATTACCCAACGTTGGCCGC
TTCCGGGTTAACGCGTTTCATCAATCGCGCGGATGCTCTGCGGTATTTCGTACCATTCCGACGGTGATCCCGACGTTAGA
TCAGCTTGAAGCTCCTGAGATTTTTACCAAGATCGCCAATTATGAAAAAGGCTTAGTGCTGGTCACTGGACCTACCGGTT
CGGGTAAATCCACCACGCTCGCGGCGATGGTCGACTACGTGAATGCTCATCACAACAAGCATATTTTGACCATTGAAGAT
CCGATTGAATTTGTACACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
CGCACTGCGCTCTGCATTGCGTGAAGACCCAGATGTGATTCTGGTTGGTGAGCTGCGTGACCAAGAAACCATTAGCTTGG
CGCTCACCGCGGCAGAAACGGGTCACTTGGTGTTTGGTACTCTGCACACCAGCTCGGCGGCGAAAACCATCGACCGTATT
ATCGACGTGTTTCCCGGTAGCGATAAAGACATGGTGCGCTCAATGCTTTCTGAATCTCTGCGTGCAGTTATCGCACAAAA
GCTACTGAAACGAGTTGGTGGTGGCCGTGTTGCTTGCCATGAAATCATGCTGGCGACTCCGGCAATCCGTAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACTGGTGCAGCACATGGTATGCAGACGATGGAGCAAAATGCG
AAGCAGCTGATTGCGCGTGGCGTAGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGATTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D2YE46

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

98.261

100

0.983
  pilT Vibrio cholerae strain A1552

98.261

100

0.983
  pilT Acinetobacter baumannii D1279779

72.024

97.391

0.701
  pilT Acinetobacter baumannii strain A118

72.024

97.391

0.701
  pilT Acinetobacter nosocomialis M2

71.726

97.391

0.699
  pilT Acinetobacter baylyi ADP1

72.121

95.652

0.69
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

66.471

98.551

0.655
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

64.242

95.652

0.614
  pilT Neisseria gonorrhoeae MS11

63.939

95.652

0.612
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.294

94.783

0.496
  pilU Vibrio cholerae strain A1552

41.916

96.812

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment