Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AV939_RS15465 Genome accession   NZ_CP013932
Coordinates   3615096..3616139 (-) Length   347 a.a.
NCBI ID   WP_057789891.1    Uniprot ID   -
Organism   Alteromonas sp. Mac1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3610096..3621139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AV939_RS20200 - 3610177..3610341 (+) 165 WP_156454644.1 hypothetical protein -
  AV939_RS15435 (AV939_15435) - 3610494..3611222 (+) 729 WP_057789901.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AV939_RS15440 (AV939_15440) gshB 3611437..3612390 (+) 954 WP_057789899.1 glutathione synthase -
  AV939_RS15445 (AV939_15445) - 3612433..3612999 (+) 567 WP_082604836.1 YqgE/AlgH family protein -
  AV939_RS15450 (AV939_15450) ruvX 3612992..3613426 (+) 435 WP_057789897.1 Holliday junction resolvase RuvX -
  AV939_RS15455 (AV939_15455) - 3613654..3613968 (-) 315 WP_057789895.1 DUF2007 domain-containing protein -
  AV939_RS15460 (AV939_15460) pilU 3613974..3615086 (-) 1113 WP_057789893.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AV939_RS15465 (AV939_15465) pilT 3615096..3616139 (-) 1044 WP_057789891.1 type IV pilus twitching motility protein PilT Machinery gene
  AV939_RS15470 (AV939_15470) - 3616164..3616850 (+) 687 WP_057789889.1 YggS family pyridoxal phosphate-dependent enzyme -
  AV939_RS15475 (AV939_15475) proC 3616907..3617728 (+) 822 WP_057789887.1 pyrroline-5-carboxylate reductase -
  AV939_RS15480 (AV939_15480) - 3617750..3618289 (+) 540 WP_057789885.1 YggT family protein -
  AV939_RS15485 (AV939_15485) - 3618312..3618752 (+) 441 WP_057789883.1 DUF4426 domain-containing protein -
  AV939_RS15490 (AV939_15490) - 3618953..3619891 (+) 939 WP_057789880.1 transposase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38344.07 Da        Isoelectric Point: 6.5064

>NTDB_id=166251 AV939_RS15465 WP_057789891.1 3615096..3616139(-) (pilT) [Alteromonas sp. Mac1]
MDITELLAFSVKNKASDLHLSAGLPPLIRVDGEMRKLNVPALDHKQVHALIYEIMNDMQRKEYEENLETDFSFEISGLSR
FRVNAFVQNRGAAAVLRTIPNTVLTLDDLGAPEIFKEIINQPTGIVLVTGATGSGKSTTLAAMVDHINSHKREHILTIED
PIEFVHENKLSLVNQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKAMVRSMLSESLRAVISQTLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQATGMQTMDQCL
QRLLALGAISKEDAAAKSKDKQSMSNF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=166251 AV939_RS15465 WP_057789891.1 3615096..3616139(-) (pilT) [Alteromonas sp. Mac1]
GTGGATATTACCGAACTTTTGGCTTTCAGCGTTAAAAATAAAGCGTCAGATTTGCACTTATCAGCAGGTCTGCCACCTCT
TATCCGCGTAGACGGAGAAATGCGTAAGCTTAATGTACCCGCATTAGATCATAAACAAGTCCATGCACTTATTTATGAAA
TAATGAATGACATGCAACGTAAAGAATACGAAGAGAACTTGGAAACCGATTTCTCATTCGAAATTAGTGGCTTGTCGAGG
TTTCGTGTAAATGCTTTTGTACAAAATCGCGGTGCAGCAGCAGTGCTTCGAACTATCCCCAACACGGTCCTTACCCTTGA
TGATTTAGGTGCACCTGAAATATTCAAAGAAATAATCAACCAACCTACCGGAATAGTTCTAGTCACCGGCGCCACGGGGT
CGGGTAAGAGTACAACCCTTGCCGCGATGGTTGATCATATCAATTCGCACAAGCGCGAGCACATTCTCACTATTGAAGAT
CCTATCGAGTTTGTACACGAAAATAAATTGAGTTTAGTAAATCAACGAGAAGTCCATCGAGACACCCACTCTTTTAGTAA
CGCACTTCGCTCAGCATTACGTGAAGATCCGGACGTGATATTAGTGGGTGAGCTACGTGATTTAGAAACAATTCGCTTGG
CCATTTCAGCTGCGGAAACGGGGCATCTAGTCTTTGGCACCTTGCATACTAACTCTGCCCCTAAAACCATAGACCGTATT
ATCGATGTGTTCCCTGCCGAAGAAAAAGCGATGGTGCGCTCTATGTTATCTGAATCACTGAGGGCAGTTATTTCTCAAAC
CCTGCTGAAAAAAGTGGGGGGAGGGCGAATAGCAGCGCATGAAATCATGGTAGGTATTCCAGCAATTCGAAATCTTATTC
GTGAAGATAAAGTACCTCAAATGTATTCCGTTATTCAGACGGGGCAAGCTACGGGTATGCAAACTATGGATCAGTGCTTA
CAACGTTTGTTGGCACTAGGTGCAATTTCTAAAGAAGACGCCGCTGCTAAATCGAAAGACAAGCAATCGATGAGTAATTT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

75

99.135

0.744
  pilT Pseudomonas aeruginosa PAK

75.221

97.695

0.735
  pilT Acinetobacter baylyi ADP1

73.547

99.135

0.729
  pilT Acinetobacter baumannii D1279779

73.547

99.135

0.729
  pilT Acinetobacter baumannii strain A118

73.547

99.135

0.729
  pilT Acinetobacter nosocomialis M2

73.256

99.135

0.726
  pilT Legionella pneumophila strain Lp02

73.353

96.254

0.706
  pilT Legionella pneumophila strain ERS1305867

73.353

96.254

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.513

97.118

0.695
  pilT Vibrio cholerae strain A1552

71.513

97.118

0.695
  pilT Neisseria meningitidis 8013

65.029

99.712

0.648
  pilT Neisseria gonorrhoeae MS11

64.74

99.712

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.538

93.66

0.501
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Acinetobacter baylyi ADP1

41.964

96.83

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401

Multiple sequence alignment