Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AV939_RS15460 Genome accession   NZ_CP013932
Coordinates   3613974..3615086 (-) Length   370 a.a.
NCBI ID   WP_057789893.1    Uniprot ID   -
Organism   Alteromonas sp. Mac1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3608974..3620086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AV939_RS20200 - 3610177..3610341 (+) 165 WP_156454644.1 hypothetical protein -
  AV939_RS15435 (AV939_15435) - 3610494..3611222 (+) 729 WP_057789901.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AV939_RS15440 (AV939_15440) gshB 3611437..3612390 (+) 954 WP_057789899.1 glutathione synthase -
  AV939_RS15445 (AV939_15445) - 3612433..3612999 (+) 567 WP_082604836.1 YqgE/AlgH family protein -
  AV939_RS15450 (AV939_15450) ruvX 3612992..3613426 (+) 435 WP_057789897.1 Holliday junction resolvase RuvX -
  AV939_RS15455 (AV939_15455) - 3613654..3613968 (-) 315 WP_057789895.1 DUF2007 domain-containing protein -
  AV939_RS15460 (AV939_15460) pilU 3613974..3615086 (-) 1113 WP_057789893.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AV939_RS15465 (AV939_15465) pilT 3615096..3616139 (-) 1044 WP_057789891.1 type IV pilus twitching motility protein PilT Machinery gene
  AV939_RS15470 (AV939_15470) - 3616164..3616850 (+) 687 WP_057789889.1 YggS family pyridoxal phosphate-dependent enzyme -
  AV939_RS15475 (AV939_15475) proC 3616907..3617728 (+) 822 WP_057789887.1 pyrroline-5-carboxylate reductase -
  AV939_RS15480 (AV939_15480) - 3617750..3618289 (+) 540 WP_057789885.1 YggT family protein -
  AV939_RS15485 (AV939_15485) - 3618312..3618752 (+) 441 WP_057789883.1 DUF4426 domain-containing protein -
  AV939_RS15490 (AV939_15490) - 3618953..3619891 (+) 939 WP_057789880.1 transposase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40842.58 Da        Isoelectric Point: 5.4057

>NTDB_id=166250 AV939_RS15460 WP_057789893.1 3613974..3615086(-) (pilU) [Alteromonas sp. Mac1]
MLDSFLEKMVEKSASDLFVTAGFPVSAKVHGKLTPISENSFNDEDALALVHEAMNEKQKEAFHSTKECNFAIVREGLGRF
RCSAFWQRDQAGMVVRRIVTEIPKVDDLGLPTVLKDIIMSKRGLVLFVGGTGTGKSTSLAALIGHRNSNSFGHILTIEDP
IEFVHEHKSCVITQREVGIDTHSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSQNIKAIVAQQLLPTKDGEGRVAAIEILLNSPLVSDLIQRNEIGSLKEAMKKGKELGMQSFDMAL
YDLYREGSIDLDQALHHADSPNDLRLMIKLDSNDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=166250 AV939_RS15460 WP_057789893.1 3613974..3615086(-) (pilU) [Alteromonas sp. Mac1]
ATGTTAGATAGCTTTTTAGAAAAAATGGTAGAGAAATCTGCTTCTGACTTATTCGTTACCGCGGGCTTTCCCGTTAGCGC
GAAAGTGCACGGTAAGCTAACCCCCATTTCGGAAAATTCTTTTAATGACGAAGATGCATTAGCACTTGTTCATGAAGCTA
TGAATGAAAAGCAAAAAGAGGCTTTTCATAGTACCAAGGAATGTAATTTTGCCATTGTACGAGAAGGCCTAGGTCGTTTT
CGTTGTTCAGCGTTTTGGCAAAGAGATCAAGCTGGGATGGTGGTACGTCGTATTGTTACCGAAATACCTAAGGTTGATGA
CCTGGGTTTACCCACGGTGCTAAAAGATATCATCATGTCAAAACGTGGCTTGGTGCTTTTTGTAGGAGGTACCGGTACAG
GTAAATCGACCTCTTTGGCGGCCTTAATCGGCCACCGAAATAGTAATTCTTTCGGACATATTCTAACTATTGAAGATCCT
ATTGAGTTTGTGCACGAGCATAAATCTTGCGTGATAACTCAGCGTGAAGTAGGCATAGACACCCACTCATTTGACGATGC
ATTGAAAAGCTCGCTGAGACAAGCGCCTGACGTCATTCTTATTGGTGAAATTCGTTCAATGGAGACAATGGAATACGCCA
TGTCGTTCGCTGATACGGGTCATTTGTGCGTAGCAACCCTCCATGCCAACAATGCTAACCAAGCAATTGAGCGTATTATG
CACTTAGCGCCAAAAGATCAGCACGACAAGTTACGTTTTGACCTAAGCCAAAATATAAAAGCTATCGTGGCGCAACAGCT
TCTTCCAACCAAAGACGGTGAGGGACGGGTAGCGGCAATTGAAATTCTATTAAACTCTCCCTTAGTGAGCGATCTTATTC
AACGTAATGAAATAGGTAGCTTGAAAGAAGCGATGAAAAAGGGCAAAGAGTTAGGAATGCAAAGCTTTGATATGGCGTTG
TACGACTTATATCGCGAGGGAAGTATTGATTTAGACCAAGCATTACATCACGCTGATTCTCCTAATGATTTGCGCCTAAT
GATTAAGCTAGATTCCAACGATGGGTCAAGCTTAGGCACATTGTCGAATGTCTCGATAGATATGGATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(105-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.299

100

0.595
  pilU Vibrio cholerae strain A1552

55.707

99.459

0.554
  pilU Acinetobacter baylyi ADP1

56.164

98.649

0.554
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.21

95.405

0.403
  pilT Acinetobacter baumannii D1279779

41.265

89.73

0.37
  pilT Acinetobacter nosocomialis M2

41.265

89.73

0.37
  pilT Acinetobacter baumannii strain A118

41.265

89.73

0.37
  pilT Pseudomonas stutzeri DSM 10701

40.12

90.27

0.362
  pilT Legionella pneumophila strain Lp02

41.875

86.486

0.362
  pilT Legionella pneumophila strain ERS1305867

41.875

86.486

0.362
  pilT Acinetobacter baylyi ADP1

40.361

89.73

0.362

Multiple sequence alignment