Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RD2015_RS04555 Genome accession   NZ_CP013729
Coordinates   1036821..1037957 (+) Length   378 a.a.
NCBI ID   WP_058933889.1    Uniprot ID   -
Organism   Roseateles depolymerans strain KCTC 42856     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1031821..1042957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RD2015_RS04530 (RD2015_915) - 1031844..1032458 (+) 615 WP_147307102.1 hypothetical protein -
  RD2015_RS04535 (RD2015_916) - 1032565..1032906 (-) 342 WP_058933885.1 energy transducer TonB -
  RD2015_RS04540 (RD2015_917) - 1032975..1034519 (-) 1545 WP_058933886.1 methyl-accepting chemotaxis protein -
  RD2015_RS04545 (RD2015_918) - 1034857..1035591 (-) 735 WP_058933887.1 YggS family pyridoxal phosphate-dependent enzyme -
  RD2015_RS04550 (RD2015_919) pilT 1035617..1036660 (+) 1044 WP_058933888.1 type IV pilus twitching motility protein PilT Machinery gene
  RD2015_RS04555 (RD2015_920) pilU 1036821..1037957 (+) 1137 WP_058933889.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RD2015_RS04560 (RD2015_921) - 1038029..1038931 (+) 903 WP_058933890.1 NAD(P)-dependent oxidoreductase -
  RD2015_RS04565 (RD2015_922) - 1039021..1039641 (-) 621 WP_058933891.1 BON domain-containing protein -
  RD2015_RS04570 (RD2015_923) - 1039745..1040314 (-) 570 WP_058933892.1 SIS domain-containing protein -
  RD2015_RS04575 (RD2015_924) - 1040340..1040723 (-) 384 WP_058937196.1 YraN family protein -
  RD2015_RS04580 (RD2015_925) rsmI 1040722..1041690 (+) 969 WP_058937197.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42116.40 Da        Isoelectric Point: 6.5937

>NTDB_id=164525 RD2015_RS04555 WP_058933889.1 1036821..1037957(+) (pilU) [Roseateles depolymerans strain KCTC 42856]
MERDQATKFINDLLRLMVSRKGSDLFLTAEFPPAIKVDGKVTKVSPQPLTAQHTLQLARSIMNDKQAADFERTKECNFAI
SPQGIGRFRVNAYIQQGAVALVLRTIPATLPTIESLELPAILKDVVQTKRGLVILVGATGSGKSTSLAAMIDHRNETTYG
HIITIEDPVEFVHPHKNCIISQREVGLDTEGWESALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKAIVSQRLLPRQEGKGRVAAVEILLNTPLISDLIFKGEVGEIKEIMKRSRELG
MQTFDQALFDLYEWGRVTYEDALRNADSVNDLRLQIKLNSERARSGDLSSGTEHLSIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=164525 RD2015_RS04555 WP_058933889.1 1036821..1037957(+) (pilU) [Roseateles depolymerans strain KCTC 42856]
ATGGAACGCGACCAGGCTACAAAGTTCATCAACGACCTGCTGCGCCTGATGGTGTCGCGCAAGGGCTCCGACCTGTTCCT
GACCGCCGAGTTCCCGCCCGCCATCAAGGTGGACGGCAAGGTGACCAAGGTGTCCCCGCAGCCGCTGACGGCCCAGCACA
CGCTGCAGCTGGCGCGTTCGATCATGAACGACAAGCAGGCGGCCGACTTCGAACGCACCAAGGAATGCAACTTCGCCATT
TCCCCGCAAGGCATTGGTCGCTTCCGGGTGAATGCCTACATCCAGCAGGGCGCGGTCGCCCTGGTGCTGCGGACCATTCC
GGCTACGTTGCCCACCATTGAGTCGCTGGAGCTGCCCGCCATCCTCAAGGACGTGGTGCAGACCAAGCGCGGCCTGGTCA
TCCTGGTGGGCGCCACCGGCTCGGGCAAGAGCACCTCGCTGGCCGCGATGATCGACCATCGCAACGAAACCACCTACGGC
CACATCATCACGATTGAAGACCCGGTGGAATTCGTGCACCCGCACAAGAACTGCATCATCAGCCAGCGGGAAGTCGGCCT
GGACACCGAGGGCTGGGAATCCGCCCTGAAGAACACGCTGCGCCAGGCGCCGGACGTCATCCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAACATGCGGTGGCCTTCGCTGAAACCGGTCACCTGTGCATGGCAACCCTGCACGCCAACAGTGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCGCCCAACTGCTGATGGACTTGTCGCTGAACCT
CAAGGCCATCGTGTCGCAGCGCCTGCTGCCGCGCCAGGAGGGCAAGGGCCGGGTGGCTGCCGTGGAGATCCTGCTGAACA
CGCCGCTGATCTCCGACCTCATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAAATCATGAAGCGCTCGCGCGAGCTGGGC
ATGCAGACCTTCGACCAAGCGCTGTTCGACCTGTACGAATGGGGCCGCGTGACCTACGAGGACGCCCTGCGCAATGCCGA
CTCGGTGAACGACCTGCGCCTGCAGATCAAGCTCAACAGCGAACGGGCGCGCAGCGGCGACCTGTCCTCCGGCACGGAGC
ATCTGTCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

57.939

94.974

0.55
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.478

89.153

0.423
  pilT Pseudomonas aeruginosa PAK

44.807

89.153

0.399
  pilT Acinetobacter nosocomialis M2

44.444

88.095

0.392
  pilT Acinetobacter baumannii D1279779

44.444

88.095

0.392
  pilT Acinetobacter baumannii strain A118

44.444

88.095

0.392
  pilT Acinetobacter baylyi ADP1

45.092

86.243

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Legionella pneumophila strain ERS1305867

44.41

85.185

0.378
  pilT Legionella pneumophila strain Lp02

44.41

85.185

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.072

87.831

0.378
  pilT Vibrio cholerae strain A1552

43.072

87.831

0.378
  pilT Neisseria gonorrhoeae MS11

40.708

89.683

0.365
  pilT Neisseria meningitidis 8013

40.708

89.683

0.365

Multiple sequence alignment