Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RD2015_RS04550 Genome accession   NZ_CP013729
Coordinates   1035617..1036660 (+) Length   347 a.a.
NCBI ID   WP_058933888.1    Uniprot ID   -
Organism   Roseateles depolymerans strain KCTC 42856     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1030617..1041660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RD2015_RS04520 - 1030747..1031397 (-) 651 WP_058933882.1 hypothetical protein -
  RD2015_RS04525 (RD2015_914) - 1031532..1031837 (+) 306 WP_232309925.1 hypothetical protein -
  RD2015_RS04530 (RD2015_915) - 1031844..1032458 (+) 615 WP_147307102.1 hypothetical protein -
  RD2015_RS04535 (RD2015_916) - 1032565..1032906 (-) 342 WP_058933885.1 energy transducer TonB -
  RD2015_RS04540 (RD2015_917) - 1032975..1034519 (-) 1545 WP_058933886.1 methyl-accepting chemotaxis protein -
  RD2015_RS04545 (RD2015_918) - 1034857..1035591 (-) 735 WP_058933887.1 YggS family pyridoxal phosphate-dependent enzyme -
  RD2015_RS04550 (RD2015_919) pilT 1035617..1036660 (+) 1044 WP_058933888.1 type IV pilus twitching motility protein PilT Machinery gene
  RD2015_RS04555 (RD2015_920) pilU 1036821..1037957 (+) 1137 WP_058933889.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RD2015_RS04560 (RD2015_921) - 1038029..1038931 (+) 903 WP_058933890.1 NAD(P)-dependent oxidoreductase -
  RD2015_RS04565 (RD2015_922) - 1039021..1039641 (-) 621 WP_058933891.1 BON domain-containing protein -
  RD2015_RS04570 (RD2015_923) - 1039745..1040314 (-) 570 WP_058933892.1 SIS domain-containing protein -
  RD2015_RS04575 (RD2015_924) - 1040340..1040723 (-) 384 WP_058937196.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38175.81 Da        Isoelectric Point: 7.0222

>NTDB_id=164524 RD2015_RS04550 WP_058933888.1 1035617..1036660(+) (pilT) [Roseateles depolymerans strain KCTC 42856]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEPLEHRQVHDMVYDIMSDAQRKAYEETLECDFSFEIQGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLENLNAPKVFAELALKPRGMVLVTGPTGSGKSTTLAGMVNHLNENEYGHILTIED
PIEFVHESKKCLVNQREVGPQTMSFSNALRSALREDPDAILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
VDVFPAAEKDMVRAMLSESLVAVISQTLCKTKDGSGRVAAHEIMLGTSAIRNLIRENKVAQMYSAIQTGNSLGMQTLDQN
LTELVRRNVISPAEARAKAKFPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=164524 RD2015_RS04550 WP_058933888.1 1035617..1036660(+) (pilT) [Roseateles depolymerans strain KCTC 42856]
ATGGACATCACTCAACTGCTGGCATTCTCGGTCAAGAACAAGGCCTCTGACCTCCACCTCTCGGCCGGACTGCCGCCCAT
GATCCGGGTGCATGGAGATGTGCGCCGCATCAATGTCGAGCCGCTCGAGCACCGTCAGGTGCACGACATGGTGTACGACA
TCATGAGCGATGCACAGCGCAAGGCCTATGAAGAGACGCTGGAATGCGACTTCTCCTTCGAGATCCAGGGCCTGGCCCGC
TTTCGCGTCAACGCGTTCAACCAGAACCGCGGTGCCGGCGCGGTCTTCCGGACCATTCCGTCCAAGATCCTGACGCTGGA
GAACCTGAACGCGCCCAAGGTGTTCGCTGAGCTGGCCCTCAAGCCGCGTGGCATGGTGCTGGTGACTGGCCCGACGGGCT
CCGGCAAGAGCACGACGCTGGCCGGCATGGTGAACCACCTCAATGAAAACGAATACGGCCACATCCTCACCATTGAGGAC
CCGATCGAATTCGTGCATGAATCCAAGAAGTGCCTGGTCAACCAGCGGGAAGTCGGTCCGCAGACCATGAGCTTCTCCAA
CGCCCTGCGCTCTGCGCTGCGCGAAGACCCGGACGCGATCCTGGTCGGTGAAATGCGGGACCTGGAAACCATCCGCCTGG
CCCTGACCGCCGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCCTCGGCCGCCAAGACGGTTGACCGGATC
GTGGACGTGTTCCCCGCCGCAGAAAAAGACATGGTCCGCGCGATGCTGTCGGAATCGCTGGTGGCGGTGATCTCCCAGAC
CCTGTGCAAGACCAAGGACGGCAGCGGCCGTGTCGCCGCCCACGAGATCATGCTGGGCACCTCGGCCATCCGCAACCTGA
TCCGTGAGAACAAGGTCGCCCAGATGTATTCGGCCATCCAGACCGGCAACAGCCTGGGCATGCAGACGCTGGACCAGAAC
CTGACCGAATTGGTGCGCCGCAATGTCATCTCGCCGGCCGAGGCCCGCGCCAAGGCCAAGTTCCCCGAGAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

72.754

99.424

0.723
  pilT Legionella pneumophila strain Lp02

71.091

97.695

0.695
  pilT Legionella pneumophila strain ERS1305867

71.091

97.695

0.695
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Neisseria gonorrhoeae MS11

67.536

99.424

0.671
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.16

97.406

0.654
  pilT Vibrio cholerae strain A1552

67.16

97.406

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409
  pilU Vibrio cholerae strain A1552

42.09

96.542

0.406

Multiple sequence alignment