Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ASZ85_RS02495 Genome accession   NZ_CP013317
Coordinates   500947..502053 (+) Length   368 a.a.
NCBI ID   WP_000422572.1    Uniprot ID   -
Organism   Vibrio cholerae strain NCTC5395     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 495947..507053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASZ85_RS02460 (ASZ85_00486) - 496238..496840 (-) 603 WP_000725017.1 XTP/dITP diphosphatase -
  ASZ85_RS02465 (ASZ85_00487) - 496910..497341 (-) 432 WP_001233676.1 DUF4426 domain-containing protein -
  ASZ85_RS02470 (ASZ85_00488) yggU 497387..497677 (-) 291 WP_000741918.1 DUF167 family protein YggU -
  ASZ85_RS02475 (ASZ85_00489) - 497677..498234 (-) 558 WP_001087261.1 YggT family protein -
  ASZ85_RS02480 (ASZ85_00490) proC 498288..499106 (-) 819 WP_000437958.1 pyrroline-5-carboxylate reductase -
  ASZ85_RS02485 (ASZ85_00491) - 499159..499869 (-) 711 WP_001256646.1 YggS family pyridoxal phosphate-dependent enzyme -
  ASZ85_RS02490 (ASZ85_00492) pilT 499895..500932 (+) 1038 WP_000350196.1 type IV pilus twitching motility protein PilT Machinery gene
  ASZ85_RS02495 (ASZ85_00493) pilU 500947..502053 (+) 1107 WP_000422572.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ASZ85_RS02500 (ASZ85_00494) - 502171..502968 (+) 798 WP_001123226.1 helix-turn-helix transcriptional regulator -
  ASZ85_RS02505 (ASZ85_00495) tyrS 503077..504357 (+) 1281 WP_001088155.1 tyrosine--tRNA ligase -
  ASZ85_RS02510 (ASZ85_00496) ruvX 504395..504817 (-) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  ASZ85_RS02515 (ASZ85_00497) - 504898..505461 (-) 564 WP_001054768.1 YqgE/AlgH family protein -
  ASZ85_RS02520 (ASZ85_00498) gshB 505497..506453 (-) 957 WP_000593313.1 glutathione synthase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41307.27 Da        Isoelectric Point: 6.5076

>NTDB_id=161994 ASZ85_RS02495 WP_000422572.1 500947..502053(+) (pilU) [Vibrio cholerae strain NCTC5395]
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=161994 ASZ85_RS02495 WP_000422572.1 500947..502053(+) (pilU) [Vibrio cholerae strain NCTC5395]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCACAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAAGCGAACTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTCAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAACT
GAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTTGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCCATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAAGTCGGGCTTGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGTGAAATCCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACGGGGCATTTGTGTATGGCCACGCTGCACGCTAACAATGCTAACCAAGCGTTAGAGCGGATTTTGCAT
TTAGTGCCGAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAGTGTTGCTCAATAGCCCGCGTATCGCGGATTTAATTCGCC
GCGGTGAACTGCATGAGCTGAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACTTTCGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

100

100

1
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Vibrio cholerae strain A1552

42.216

90.761

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

90.761

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

42.462

88.315

0.375
  pilT Neisseria gonorrhoeae MS11

38.329

94.293

0.361
  pilT Neisseria meningitidis 8013

38.551

93.75

0.361

Multiple sequence alignment