Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ASZ85_RS02490 Genome accession   NZ_CP013317
Coordinates   499895..500932 (+) Length   345 a.a.
NCBI ID   WP_000350196.1    Uniprot ID   A0A0K9UNT8
Organism   Vibrio cholerae strain NCTC5395     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 494895..505932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASZ85_RS02455 (ASZ85_00485) hemW 495069..496244 (-) 1176 WP_000957338.1 radical SAM family heme chaperone HemW -
  ASZ85_RS02460 (ASZ85_00486) - 496238..496840 (-) 603 WP_000725017.1 XTP/dITP diphosphatase -
  ASZ85_RS02465 (ASZ85_00487) - 496910..497341 (-) 432 WP_001233676.1 DUF4426 domain-containing protein -
  ASZ85_RS02470 (ASZ85_00488) yggU 497387..497677 (-) 291 WP_000741918.1 DUF167 family protein YggU -
  ASZ85_RS02475 (ASZ85_00489) - 497677..498234 (-) 558 WP_001087261.1 YggT family protein -
  ASZ85_RS02480 (ASZ85_00490) proC 498288..499106 (-) 819 WP_000437958.1 pyrroline-5-carboxylate reductase -
  ASZ85_RS02485 (ASZ85_00491) - 499159..499869 (-) 711 WP_001256646.1 YggS family pyridoxal phosphate-dependent enzyme -
  ASZ85_RS02490 (ASZ85_00492) pilT 499895..500932 (+) 1038 WP_000350196.1 type IV pilus twitching motility protein PilT Machinery gene
  ASZ85_RS02495 (ASZ85_00493) pilU 500947..502053 (+) 1107 WP_000422572.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ASZ85_RS02500 (ASZ85_00494) - 502171..502968 (+) 798 WP_001123226.1 helix-turn-helix transcriptional regulator -
  ASZ85_RS02505 (ASZ85_00495) tyrS 503077..504357 (+) 1281 WP_001088155.1 tyrosine--tRNA ligase -
  ASZ85_RS02510 (ASZ85_00496) ruvX 504395..504817 (-) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  ASZ85_RS02515 (ASZ85_00497) - 504898..505461 (-) 564 WP_001054768.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38249.86 Da        Isoelectric Point: 6.6672

>NTDB_id=161993 ASZ85_RS02490 WP_000350196.1 499895..500932(+) (pilT) [Vibrio cholerae strain NCTC5395]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=161993 ASZ85_RS02490 WP_000350196.1 499895..500932(+) (pilT) [Vibrio cholerae strain NCTC5395]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTACCTGCTTTTACCCATTCTGATGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTTATCCCGACGTTAGA
GCAACTGGATGCTCCTGAGATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGGT
CGGGTAAGTCGACAACCTTGGCGGCGATGGTGAACTATGTCAATGCCCATCACAATAAGCATATTTTGACTATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATTTTGGTTGGTGAGCTGCGTGACCAAGAAACCATTAGCTTGG
CGCTTACTGCGGCAGAAACCGGTCACTTGGTGTTTGGCACTCTACACACCAGCTCGGCGGCGAAAACCATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGTTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
ACTCCTAAAACGCGTCGGGGGTGGCCGTGTCGCGTGTCATGAAATCATGCTGGCGACGCCAGCGATCCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACCATGGAGCAAAATGCC
AAACAGCTGATGGCACGTGGTGTGGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGATTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K9UNT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

99.71

100

0.997
  pilT Vibrio cholerae strain A1552

99.71

100

0.997
  pilT Acinetobacter baumannii D1279779

71.726

97.391

0.699
  pilT Acinetobacter baumannii strain A118

71.726

97.391

0.699
  pilT Acinetobacter nosocomialis M2

71.429

97.391

0.696
  pilT Acinetobacter baylyi ADP1

72.424

95.652

0.693
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

66.765

98.551

0.658
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

64.545

95.652

0.617
  pilT Neisseria gonorrhoeae MS11

64.242

95.652

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.988

94.783

0.493
  pilU Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.828

97.971

0.4
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment