Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CPter291_RS03785 Genome accession   NZ_CP013236
Coordinates   842880..843923 (-) Length   347 a.a.
NCBI ID   WP_061937271.1    Uniprot ID   A0A127QSW7
Organism   Collimonas pratensis strain Ter291     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 837880..848923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPter291_RS03765 (CPter291_0788) glpK 838751..840247 (+) 1497 WP_061937260.1 glycerol kinase GlpK -
  CPter291_RS03770 (CPter291_0789) - 840342..841055 (+) 714 WP_061937263.1 MIP/aquaporin family protein -
  CPter291_RS03775 (CPter291_0790) - 841120..841620 (-) 501 WP_062112072.1 glutathione peroxidase -
  CPter291_RS03780 (CPter291_0791) pilU 841617..842753 (-) 1137 WP_061937269.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CPter291_RS03785 (CPter291_0792) pilT 842880..843923 (-) 1044 WP_061937271.1 type IV pilus twitching motility protein PilT Machinery gene
  CPter291_RS03790 (CPter291_0793) - 844109..844855 (+) 747 WP_062112074.1 YggS family pyridoxal phosphate-dependent enzyme -
  CPter291_RS03795 (CPter291_0794) proC 844858..845682 (+) 825 WP_062112076.1 pyrroline-5-carboxylate reductase -
  CPter291_RS03800 (CPter291_0795) - 845842..846162 (-) 321 WP_061937280.1 DUF883 family protein -
  CPter291_RS03805 (CPter291_0796) ubiA 846233..847087 (-) 855 WP_061937283.1 4-hydroxybenzoate octaprenyltransferase -
  CPter291_RS03810 (CPter291_0797) - 847185..848132 (-) 948 WP_061937286.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38055.62 Da        Isoelectric Point: 6.6929

>NTDB_id=160940 CPter291_RS03785 WP_061937271.1 842880..843923(-) (pilT) [Collimonas pratensis strain Ter291]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHSMIYDIMNDGQRKAFEEHLECDFSFEIPGLAR
FRVNAFNQDRGAAAVLRTIPSTVLTLEQLNAPRIFAELSLKPRGLVLVTGPTGSGKSTTLAAMVNHVNENEYAHILTIED
PIEFVHQSNKCLINQREVGPHTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRAMLSESLQAVISQSLLKTKDGSGRVAAHEIMLGTPAIRNLIRESKIAQMYSAIQTGSNMGMQTLDSN
LTDLVKRNVISLATARAAAKTPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=160940 CPter291_RS03785 WP_061937271.1 842880..843923(-) (pilT) [Collimonas pratensis strain Ter291]
ATGGACATTTCCGAACTTCTGGCTTTTTCGGTGAAGAACAAGGCATCCGATTTGCATCTATCCGCAGGCTTGCCGCCGAT
GATCCGGGTTCATGGAGATGTGCGCCGCATCAACCTGCCGCCGCTCGAGCACAAAGACGTGCACTCGATGATCTATGACA
TCATGAACGACGGCCAGCGCAAGGCTTTCGAAGAACACCTGGAGTGCGATTTCTCGTTCGAGATCCCGGGCCTGGCGCGC
TTTCGCGTCAATGCCTTCAACCAGGACCGCGGCGCTGCCGCCGTATTGCGGACCATTCCTTCCACCGTGCTGACGCTGGA
ACAGCTCAACGCGCCGCGCATCTTTGCCGAACTGTCGCTCAAGCCACGTGGCCTGGTGCTGGTCACCGGCCCGACCGGCT
CCGGCAAATCGACCACGCTGGCGGCGATGGTCAACCACGTCAATGAAAACGAATACGCCCACATCCTGACCATCGAAGAT
CCGATCGAATTCGTCCATCAGTCCAACAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCGCATACCCATTCGTTCAGCAA
TGCGCTGCGCTCGGCCTTGCGTGAAGACCCGGATGTCATCCTGGTGGGCGAGTTGCGCGACCTGGAGACCATCCGCCTGG
CCATGACTGCCGCCGAAACCGGTCACCTGGTGTTCGGCACCTTGCACACATCCTCCGCCGCCAAGACCATCGACCGTATC
GTCGATGTCTTTCCGGCGGAAGAAAAAGAAATGGTGCGCGCCATGCTGTCGGAATCGCTGCAGGCGGTGATTTCGCAAAG
CCTGCTGAAAACCAAGGATGGCTCCGGCCGCGTCGCCGCCCATGAAATCATGCTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAAAGCAAGATCGCCCAGATGTATTCGGCGATCCAGACCGGCAGCAACATGGGCATGCAAACCCTGGACAGCAAC
CTGACCGACCTGGTCAAGCGCAACGTGATTTCGCTGGCGACAGCGCGCGCCGCGGCCAAGACGCCGGATAATTTCCCGGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127QSW7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.101

99.424

0.767
  pilT Acinetobacter baumannii D1279779

77.101

99.424

0.767
  pilT Acinetobacter baumannii strain A118

77.101

99.424

0.767
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

75.221

97.695

0.735
  pilT Legionella pneumophila strain ERS1305867

75.221

97.695

0.735
  pilT Acinetobacter baylyi ADP1

73.623

99.424

0.732
  pilT Neisseria meningitidis 8013

68.696

99.424

0.683
  pilT Neisseria gonorrhoeae MS11

68.406

99.424

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.909

95.101

0.674
  pilT Vibrio cholerae strain A1552

70.909

95.101

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

99.424

0.516
  pilU Pseudomonas stutzeri DSM 10701

40.87

99.424

0.406
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406
  pilU Vibrio cholerae strain A1552

40

99.424

0.398

Multiple sequence alignment