Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CPter291_RS03780 Genome accession   NZ_CP013236
Coordinates   841617..842753 (-) Length   378 a.a.
NCBI ID   WP_061937269.1    Uniprot ID   A0A2A9KH81
Organism   Collimonas pratensis strain Ter291     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 836617..847753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPter291_RS03760 (CPter291_0787) glpD 837109..838656 (+) 1548 WP_062112070.1 glycerol-3-phosphate dehydrogenase -
  CPter291_RS03765 (CPter291_0788) glpK 838751..840247 (+) 1497 WP_061937260.1 glycerol kinase GlpK -
  CPter291_RS03770 (CPter291_0789) - 840342..841055 (+) 714 WP_061937263.1 MIP/aquaporin family protein -
  CPter291_RS03775 (CPter291_0790) - 841120..841620 (-) 501 WP_062112072.1 glutathione peroxidase -
  CPter291_RS03780 (CPter291_0791) pilU 841617..842753 (-) 1137 WP_061937269.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CPter291_RS03785 (CPter291_0792) pilT 842880..843923 (-) 1044 WP_061937271.1 type IV pilus twitching motility protein PilT Machinery gene
  CPter291_RS03790 (CPter291_0793) - 844109..844855 (+) 747 WP_062112074.1 YggS family pyridoxal phosphate-dependent enzyme -
  CPter291_RS03795 (CPter291_0794) proC 844858..845682 (+) 825 WP_062112076.1 pyrroline-5-carboxylate reductase -
  CPter291_RS03800 (CPter291_0795) - 845842..846162 (-) 321 WP_061937280.1 DUF883 family protein -
  CPter291_RS03805 (CPter291_0796) ubiA 846233..847087 (-) 855 WP_061937283.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42034.40 Da        Isoelectric Point: 6.4216

>NTDB_id=160939 CPter291_RS03780 WP_061937269.1 841617..842753(-) (pilU) [Collimonas pratensis strain Ter291]
MERDQATKFMHDLLRLMVSKRGSDLFITADFPPAFKIDGKITPVSNQALTAVHTLELARAIMSDKQAADFEETKECNFAI
SPGGLGRFRASAFIQQGRVGMVLRTITTDIPKFEDLGLPPTLKEVAMTKRGLVIMVGATGSGKSTTLAAMIGYRNENSYG
HIITIEDPVEYIHPHKNCIVTQREVGVDTENWGVALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCLATLHANSS
NQALDRIINFFPEERRPQLLMDLSLNLKGIVSQRLVPLKTVKGRAVAVEILLNSPLISDLIFKGDVHEIKEIMKKSRELG
MQTFDQALFDLYEDDKISYEDALRNADSVNDLRLSIKLRGKDAKDRDLSAGTEHLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=160939 CPter291_RS03780 WP_061937269.1 841617..842753(-) (pilU) [Collimonas pratensis strain Ter291]
ATGGAACGCGATCAAGCAACCAAATTCATGCACGACCTGCTGCGCCTGATGGTCAGCAAGCGAGGCTCCGATCTGTTCAT
CACCGCAGACTTCCCGCCGGCCTTCAAGATCGACGGCAAGATCACGCCGGTCTCGAACCAGGCGCTGACCGCCGTCCATA
CGCTGGAACTGGCGCGCGCCATCATGAGCGACAAGCAGGCTGCGGATTTCGAAGAAACCAAGGAATGCAATTTCGCCATC
AGCCCTGGCGGCCTGGGGCGCTTCCGCGCTTCCGCCTTCATCCAGCAGGGCCGGGTCGGCATGGTGTTGCGGACGATTAC
CACCGACATCCCTAAATTCGAAGACCTGGGCCTGCCGCCGACGCTCAAGGAAGTCGCCATGACCAAGCGCGGCCTGGTGA
TCATGGTCGGCGCCACCGGTTCCGGCAAATCGACCACGCTGGCGGCGATGATCGGCTACCGCAACGAAAACAGCTACGGT
CATATCATCACGATTGAAGATCCGGTCGAATACATCCATCCGCACAAGAACTGCATCGTGACCCAGCGCGAAGTCGGCGT
CGATACCGAAAACTGGGGCGTGGCCCTGAAGAACACCTTGCGCCAGGCGCCAGACGTGATCCTGATCGGTGAAATCCGCG
ACCGTGAAACCATGGACTACGCCATCGCTTTCGCCGAAACCGGGCACTTGTGCCTGGCCACGCTGCATGCCAACAGCTCC
AACCAGGCGCTCGACCGTATCATCAACTTTTTCCCGGAAGAACGCCGGCCGCAACTGCTGATGGACTTGTCGCTCAATCT
TAAAGGGATCGTCTCGCAACGCCTAGTACCGCTGAAAACGGTCAAGGGCCGTGCGGTCGCGGTGGAGATCCTGCTGAACT
CACCTTTGATTTCCGACCTGATCTTCAAGGGCGATGTCCATGAAATCAAGGAAATCATGAAGAAGTCGCGCGAGCTCGGC
ATGCAGACTTTTGACCAGGCCCTGTTCGATTTGTACGAAGACGATAAAATCAGCTATGAAGACGCTTTGCGCAATGCCGA
TTCGGTCAACGACCTGCGGCTTTCCATCAAACTGCGCGGCAAGGACGCAAAAGACCGCGACCTGTCTGCCGGCACTGAAC
ATCTGGGGATCGTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A9KH81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.474

91.534

0.608
  pilU Acinetobacter baylyi ADP1

60.286

92.593

0.558
  pilU Vibrio cholerae strain A1552

57.637

91.799

0.529
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.582

88.624

0.386
  pilT Legionella pneumophila strain Lp02

41.692

87.566

0.365
  pilT Legionella pneumophila strain ERS1305867

41.692

87.566

0.365
  pilT Pseudomonas aeruginosa PAK

41.194

88.624

0.365

Multiple sequence alignment