Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CAter282_RS03365 Genome accession   NZ_CP013235
Coordinates   717334..718377 (-) Length   347 a.a.
NCBI ID   WP_061532286.1    Uniprot ID   A0A127QEV5
Organism   Collimonas arenae strain Ter282     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 712334..723377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAter282_RS03345 (CAter282_0719) glpK 713230..714726 (+) 1497 WP_061532282.1 glycerol kinase GlpK -
  CAter282_RS03350 (CAter282_0720) - 714830..715543 (+) 714 WP_061532283.1 MIP/aquaporin family protein -
  CAter282_RS03355 (CAter282_0721) - 715629..716126 (-) 498 WP_061532284.1 glutathione peroxidase -
  CAter282_RS03360 (CAter282_0722) pilU 716123..717259 (-) 1137 WP_061532285.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CAter282_RS03365 (CAter282_0723) pilT 717334..718377 (-) 1044 WP_061532286.1 type IV pilus twitching motility protein PilT Machinery gene
  CAter282_RS03370 (CAter282_0724) - 718557..719300 (+) 744 WP_061532287.1 YggS family pyridoxal phosphate-dependent enzyme -
  CAter282_RS03375 (CAter282_0725) proC 719314..720138 (+) 825 WP_061532288.1 pyrroline-5-carboxylate reductase -
  CAter282_RS03380 (CAter282_0726) - 720277..720597 (-) 321 WP_061532289.1 DUF883 family protein -
  CAter282_RS03385 (CAter282_0727) ubiA 720669..721523 (-) 855 WP_061532290.1 4-hydroxybenzoate octaprenyltransferase -
  CAter282_RS03390 (CAter282_0728) - 721658..722605 (-) 948 WP_061536965.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38036.56 Da        Isoelectric Point: 6.5672

>NTDB_id=160918 CAter282_RS03365 WP_061532286.1 717334..718377(-) (pilT) [Collimonas arenae strain Ter282]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPALEHQEVHAMIYDIMNDGQRKAYEEHLEVDFSFEIPGLAR
FRVNAFNQDRGAAAVLRTIPSTVLTLEQLNAPRIFAELSLKPRGLVLVTGPTGSGKSTTLAAMVNHVNENEYAHILTIED
PIEFVHQSNKCLINQREVGPHTHSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRAMLSESLQAVISQSLLKTKDGSGRVAAHEIMLGTPAIRNLIRESKIAQMYSAIQTGSNMGMQTLDSN
LTELVKRNVISMAAARAAAKSPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=160918 CAter282_RS03365 WP_061532286.1 717334..718377(-) (pilT) [Collimonas arenae strain Ter282]
ATGGACATTTCCGAACTTCTGGCTTTTTCGGTAAAGAATAAGGCATCCGATTTGCATTTATCCGCAGGCTTGCCGCCGAT
GATCCGGGTTCATGGAGATGTACGCCGCATCAACCTGCCGGCGCTTGAGCATCAAGAAGTGCATGCGATGATCTATGACA
TCATGAACGACGGCCAGCGCAAGGCCTACGAAGAACACCTGGAAGTCGATTTTTCGTTCGAGATCCCCGGACTGGCGCGC
TTCCGTGTCAACGCATTCAACCAGGACCGCGGCGCCGCTGCCGTGCTGCGGACCATTCCGTCGACCGTGCTGACGCTGGA
ACAACTCAACGCGCCGCGCATTTTTGCCGAACTGTCACTGAAGCCGCGCGGCCTGGTGTTGGTTACCGGTCCAACTGGCT
CCGGCAAATCGACCACGCTGGCGGCGATGGTCAATCACGTCAACGAAAACGAATACGCGCACATCCTGACCATCGAAGAT
CCGATCGAATTCGTCCACCAATCCAACAAATGCCTGATCAACCAGCGTGAAGTCGGGCCGCACACGCACTCATTCAATAA
TGCGCTGCGCTCCGCATTGCGCGAAGATCCGGACGTGATTCTGGTCGGCGAATTGCGCGATCTGGAAACCATTCGTCTGG
CGCTGACCGCCGCCGAAACCGGCCATCTGGTGTTCGGCACGCTGCACACTTCCTCGGCCGCCAAGACCATCGATCGTATC
GTCGACGTGTTCCCGGCAGAAGAAAAGGAAATGGTGCGGGCGATGTTGTCCGAATCACTCCAGGCAGTGATTTCACAAAG
TCTGCTCAAGACCAAGGATGGTTCGGGCCGTGTCGCTGCCCATGAAATCATGCTGGGCACGCCGGCGATCCGTAACCTGA
TCCGCGAAAGCAAGATCGCCCAGATGTATTCGGCGATTCAGACCGGCAGCAACATGGGAATGCAGACGCTCGACAGCAAC
CTGACCGAACTGGTCAAACGCAATGTCATTTCAATGGCCGCCGCGCGCGCTGCGGCCAAATCGCCGGATAACTTCCCTGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127QEV5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.391

99.424

0.769
  pilT Acinetobacter baumannii D1279779

77.391

99.424

0.769
  pilT Acinetobacter baumannii strain A118

77.391

99.424

0.769
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

74.631

97.695

0.729
  pilT Legionella pneumophila strain ERS1305867

74.631

97.695

0.729
  pilT Neisseria gonorrhoeae MS11

69.855

99.424

0.695
  pilT Neisseria meningitidis 8013

69.855

99.424

0.695
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.818

95.101

0.683
  pilT Vibrio cholerae strain A1552

71.818

95.101

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.29

99.424

0.401
  pilU Vibrio cholerae strain A1552

40

99.424

0.398

Multiple sequence alignment