Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CAter282_RS03360 Genome accession   NZ_CP013235
Coordinates   716123..717259 (-) Length   378 a.a.
NCBI ID   WP_061532285.1    Uniprot ID   -
Organism   Collimonas arenae strain Ter282     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 711123..722259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAter282_RS03340 (CAter282_0718) glpD 711565..713109 (+) 1545 WP_061536964.1 glycerol-3-phosphate dehydrogenase -
  CAter282_RS03345 (CAter282_0719) glpK 713230..714726 (+) 1497 WP_061532282.1 glycerol kinase GlpK -
  CAter282_RS03350 (CAter282_0720) - 714830..715543 (+) 714 WP_061532283.1 MIP/aquaporin family protein -
  CAter282_RS03355 (CAter282_0721) - 715629..716126 (-) 498 WP_061532284.1 glutathione peroxidase -
  CAter282_RS03360 (CAter282_0722) pilU 716123..717259 (-) 1137 WP_061532285.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CAter282_RS03365 (CAter282_0723) pilT 717334..718377 (-) 1044 WP_061532286.1 type IV pilus twitching motility protein PilT Machinery gene
  CAter282_RS03370 (CAter282_0724) - 718557..719300 (+) 744 WP_061532287.1 YggS family pyridoxal phosphate-dependent enzyme -
  CAter282_RS03375 (CAter282_0725) proC 719314..720138 (+) 825 WP_061532288.1 pyrroline-5-carboxylate reductase -
  CAter282_RS03380 (CAter282_0726) - 720277..720597 (-) 321 WP_061532289.1 DUF883 family protein -
  CAter282_RS03385 (CAter282_0727) ubiA 720669..721523 (-) 855 WP_061532290.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42092.55 Da        Isoelectric Point: 6.4202

>NTDB_id=160917 CAter282_RS03360 WP_061532285.1 716123..717259(-) (pilU) [Collimonas arenae strain Ter282]
MERDQATKFMHDLLRLMVSKRGSDLFITADFPPAFKIDGKITPVSNQALTAVHTLELARAIMSDKQAADFEETKECNFAI
SPNGLGRFRASAFIQQGRVGMVLRVITTDIPKFEDLGLPPTLKDVAMTKRGLVIMVGATGSGKSTTLAAMVGYRNENSYG
HIITIEDPVEYIHPHKNCIVTQREVGVDTESWSVALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRPQLLMDLSLNLKGIVSQRLIPLKTVKGRCAAVEILLNSPLISDLIFKGDVHEIKEIMKKSRELG
MQTFDQALFDLYEDDKITYEDALRNADSINDLRLSIKLRGKEAKDRDLAAGTEHLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=160917 CAter282_RS03360 WP_061532285.1 716123..717259(-) (pilU) [Collimonas arenae strain Ter282]
ATGGAAAGAGATCAAGCGACCAAATTCATGCACGACTTGCTGCGCCTGATGGTCAGCAAGCGCGGCTCCGATCTGTTCAT
CACCGCCGACTTCCCGCCGGCCTTCAAGATCGACGGCAAGATCACGCCGGTGTCCAACCAGGCACTGACCGCAGTGCATA
CGCTGGAGCTGGCGCGCGCCATCATGAGTGACAAGCAAGCTGCCGACTTTGAAGAAACCAAAGAGTGCAATTTTGCGATC
AGCCCCAACGGACTGGGCCGTTTCCGCGCTTCGGCATTCATCCAGCAGGGCCGGGTCGGCATGGTGCTGCGGGTCATCAC
CACCGACATCCCGAAATTTGAAGACCTGGGCTTGCCGCCGACGTTGAAAGATGTCGCCATGACCAAGCGCGGCCTGGTGA
TCATGGTCGGCGCCACAGGCTCTGGCAAATCGACTACGCTGGCGGCTATGGTTGGCTACCGTAACGAAAACAGTTACGGC
CACATCATCACTATCGAGGACCCGGTCGAATATATCCACCCGCACAAGAACTGCATCGTTACCCAGCGCGAAGTCGGCGT
CGATACGGAAAGCTGGAGCGTGGCCTTGAAAAATACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCG
ATCGCGAAACCATGGACTACGCCATCGCCTTCGCCGAAACAGGTCACCTGTGCCTGGCTACTCTGCATGCCAACAGCGCC
AACCAGGCGCTTGACCGTATCATCAACTTCTTCCCGGAAGAGCGTCGGCCGCAATTGCTGATGGACTTGTCGCTGAACCT
GAAAGGGATCGTCTCGCAGCGGCTGATTCCGTTGAAAACCGTCAAGGGCCGCTGCGCCGCGGTCGAGATCTTGCTCAATT
CGCCGCTGATTTCCGACCTGATCTTCAAGGGCGACGTCCATGAAATCAAGGAAATCATGAAGAAGTCGCGCGAACTCGGC
ATGCAGACTTTCGACCAGGCGCTGTTCGATTTGTACGAAGACGATAAAATCACTTACGAAGACGCGTTGCGCAACGCCGA
TTCGATCAACGATTTGCGCCTGTCCATCAAGCTGCGCGGCAAAGAAGCCAAGGATCGCGACCTTGCCGCCGGCACTGAAC
ATCTGGGGATTGTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.014

96.032

0.624
  pilU Acinetobacter baylyi ADP1

59.61

94.974

0.566
  pilU Vibrio cholerae strain A1552

57.726

90.741

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.179

88.624

0.392
  pilT Pseudomonas aeruginosa PAK

41.791

88.624

0.37
  pilT Acinetobacter nosocomialis M2

41.39

87.566

0.362
  pilT Legionella pneumophila strain Lp02

41.39

87.566

0.362
  pilT Legionella pneumophila strain ERS1305867

41.39

87.566

0.362

Multiple sequence alignment