Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CPter91_RS04105 Genome accession   NZ_CP013234
Coordinates   904948..905991 (-) Length   347 a.a.
NCBI ID   WP_061937271.1    Uniprot ID   A0A127QSW7
Organism   Collimonas pratensis strain Ter91     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 899948..910991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPter91_RS04085 (CPter91_0871) glpK 900793..902289 (+) 1497 WP_061937260.1 glycerol kinase GlpK -
  CPter91_RS04090 (CPter91_0872) - 902384..903097 (+) 714 WP_061937263.1 MIP/aquaporin family protein -
  CPter91_RS04095 (CPter91_0873) - 903188..903688 (-) 501 WP_061937266.1 glutathione peroxidase -
  CPter91_RS04100 (CPter91_0874) pilU 903685..904821 (-) 1137 WP_061937269.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CPter91_RS04105 (CPter91_0875) pilT 904948..905991 (-) 1044 WP_061937271.1 type IV pilus twitching motility protein PilT Machinery gene
  CPter91_RS04110 (CPter91_0876) - 906177..906923 (+) 747 WP_061937274.1 YggS family pyridoxal phosphate-dependent enzyme -
  CPter91_RS04115 (CPter91_0877) proC 906926..907750 (+) 825 WP_061937277.1 pyrroline-5-carboxylate reductase -
  CPter91_RS04120 (CPter91_0878) - 907912..908232 (-) 321 WP_061937280.1 DUF883 family protein -
  CPter91_RS04125 (CPter91_0879) ubiA 908303..909157 (-) 855 WP_061937283.1 4-hydroxybenzoate octaprenyltransferase -
  CPter91_RS04130 (CPter91_0880) - 909255..910202 (-) 948 WP_061937286.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38055.62 Da        Isoelectric Point: 6.6929

>NTDB_id=160897 CPter91_RS04105 WP_061937271.1 904948..905991(-) (pilT) [Collimonas pratensis strain Ter91]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHSMIYDIMNDGQRKAFEEHLECDFSFEIPGLAR
FRVNAFNQDRGAAAVLRTIPSTVLTLEQLNAPRIFAELSLKPRGLVLVTGPTGSGKSTTLAAMVNHVNENEYAHILTIED
PIEFVHQSNKCLINQREVGPHTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRAMLSESLQAVISQSLLKTKDGSGRVAAHEIMLGTPAIRNLIRESKIAQMYSAIQTGSNMGMQTLDSN
LTDLVKRNVISLATARAAAKTPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=160897 CPter91_RS04105 WP_061937271.1 904948..905991(-) (pilT) [Collimonas pratensis strain Ter91]
ATGGACATTTCCGAACTTCTGGCTTTTTCGGTGAAGAACAAGGCATCCGATTTGCATCTATCCGCAGGCTTGCCGCCGAT
GATCCGGGTTCATGGAGATGTGCGCCGCATCAACTTGCCGCCGCTCGAGCACAAAGACGTGCACTCGATGATCTATGACA
TCATGAACGACGGCCAGCGCAAGGCTTTCGAAGAACACCTGGAGTGCGATTTCTCGTTCGAGATCCCGGGCCTGGCGCGC
TTTCGCGTCAATGCATTCAACCAGGACCGGGGCGCGGCCGCCGTATTGCGGACCATTCCTTCCACCGTGCTGACGCTGGA
ACAGCTCAATGCGCCGCGCATCTTTGCCGAACTGTCGCTCAAGCCACGTGGCCTGGTGCTAGTCACCGGCCCGACCGGCT
CCGGCAAATCGACCACGCTGGCGGCGATGGTCAACCACGTCAATGAAAACGAATACGCGCACATCCTGACCATTGAAGAT
CCGATCGAATTCGTCCATCAGTCCAACAAGTGCCTGATCAACCAGCGCGAAGTCGGTCCGCATACCCATTCGTTCAGCAA
TGCGCTGCGCTCGGCCTTGCGCGAAGACCCGGATGTCATCCTGGTCGGCGAGTTGCGCGACCTGGAGACCATCCGCCTGG
CCATGACTGCCGCAGAAACCGGTCACCTGGTGTTCGGCACCTTGCACACATCCTCCGCCGCCAAGACCATCGACCGTATC
GTCGACGTCTTTCCGGCGGAAGAAAAAGAAATGGTGCGCGCCATGCTGTCGGAATCGCTGCAGGCGGTGATTTCGCAAAG
CCTGCTGAAAACCAAGGATGGCTCCGGCCGCGTCGCCGCCCATGAAATCATGCTGGGCACGCCGGCGATCCGCAACCTGA
TCCGCGAAAGCAAGATCGCCCAGATGTATTCGGCGATCCAGACCGGCAGCAACATGGGCATGCAAACCCTGGACAGCAAC
CTGACCGACCTGGTCAAGCGCAACGTGATTTCGCTGGCGACAGCGCGCGCCGCGGCCAAGACGCCGGATAATTTCCCGGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127QSW7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.101

99.424

0.767
  pilT Acinetobacter baumannii D1279779

77.101

99.424

0.767
  pilT Acinetobacter baumannii strain A118

77.101

99.424

0.767
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

75.221

97.695

0.735
  pilT Legionella pneumophila strain ERS1305867

75.221

97.695

0.735
  pilT Acinetobacter baylyi ADP1

73.623

99.424

0.732
  pilT Neisseria meningitidis 8013

68.696

99.424

0.683
  pilT Neisseria gonorrhoeae MS11

68.406

99.424

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.909

95.101

0.674
  pilT Vibrio cholerae strain A1552

70.909

95.101

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

99.424

0.516
  pilU Pseudomonas stutzeri DSM 10701

40.87

99.424

0.406
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406
  pilU Vibrio cholerae strain A1552

40

99.424

0.398

Multiple sequence alignment