Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CPter91_RS04100 Genome accession   NZ_CP013234
Coordinates   903685..904821 (-) Length   378 a.a.
NCBI ID   WP_061937269.1    Uniprot ID   A0A2A9KH81
Organism   Collimonas pratensis strain Ter91     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 898685..909821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPter91_RS04080 (CPter91_0870) glpD 899151..900698 (+) 1548 WP_061937257.1 glycerol-3-phosphate dehydrogenase -
  CPter91_RS04085 (CPter91_0871) glpK 900793..902289 (+) 1497 WP_061937260.1 glycerol kinase GlpK -
  CPter91_RS04090 (CPter91_0872) - 902384..903097 (+) 714 WP_061937263.1 MIP/aquaporin family protein -
  CPter91_RS04095 (CPter91_0873) - 903188..903688 (-) 501 WP_061937266.1 glutathione peroxidase -
  CPter91_RS04100 (CPter91_0874) pilU 903685..904821 (-) 1137 WP_061937269.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CPter91_RS04105 (CPter91_0875) pilT 904948..905991 (-) 1044 WP_061937271.1 type IV pilus twitching motility protein PilT Machinery gene
  CPter91_RS04110 (CPter91_0876) - 906177..906923 (+) 747 WP_061937274.1 YggS family pyridoxal phosphate-dependent enzyme -
  CPter91_RS04115 (CPter91_0877) proC 906926..907750 (+) 825 WP_061937277.1 pyrroline-5-carboxylate reductase -
  CPter91_RS04120 (CPter91_0878) - 907912..908232 (-) 321 WP_061937280.1 DUF883 family protein -
  CPter91_RS04125 (CPter91_0879) ubiA 908303..909157 (-) 855 WP_061937283.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42034.40 Da        Isoelectric Point: 6.4216

>NTDB_id=160896 CPter91_RS04100 WP_061937269.1 903685..904821(-) (pilU) [Collimonas pratensis strain Ter91]
MERDQATKFMHDLLRLMVSKRGSDLFITADFPPAFKIDGKITPVSNQALTAVHTLELARAIMSDKQAADFEETKECNFAI
SPGGLGRFRASAFIQQGRVGMVLRTITTDIPKFEDLGLPPTLKEVAMTKRGLVIMVGATGSGKSTTLAAMIGYRNENSYG
HIITIEDPVEYIHPHKNCIVTQREVGVDTENWGVALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCLATLHANSS
NQALDRIINFFPEERRPQLLMDLSLNLKGIVSQRLVPLKTVKGRAVAVEILLNSPLISDLIFKGDVHEIKEIMKKSRELG
MQTFDQALFDLYEDDKISYEDALRNADSVNDLRLSIKLRGKDAKDRDLSAGTEHLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=160896 CPter91_RS04100 WP_061937269.1 903685..904821(-) (pilU) [Collimonas pratensis strain Ter91]
ATGGAACGCGATCAAGCAACCAAATTCATGCACGACCTGCTGCGCCTGATGGTCAGCAAACGGGGCTCAGATCTATTCAT
TACCGCGGACTTCCCGCCGGCCTTCAAGATCGACGGTAAGATCACGCCGGTCTCGAACCAGGCGCTGACCGCCGTCCATA
CGCTGGAACTGGCGCGCGCCATCATGAGCGACAAGCAGGCTGCGGATTTCGAAGAAACCAAGGAATGCAATTTCGCCATC
AGCCCTGGCGGCCTGGGGCGCTTCCGTGCTTCCGCCTTCATCCAGCAGGGCCGGGTCGGCATGGTGTTGCGGACGATTAC
CACCGACATCCCTAAATTCGAAGACCTGGGCCTGCCGCCGACGCTCAAGGAAGTCGCCATGACCAAGCGCGGCCTGGTGA
TCATGGTCGGCGCCACCGGCTCCGGCAAATCGACCACGCTGGCGGCGATGATCGGCTACCGCAACGAAAACAGCTACGGT
CATATCATCACGATTGAAGATCCGGTCGAATACATCCATCCGCACAAGAACTGTATCGTGACCCAGCGCGAAGTCGGCGT
CGATACCGAAAACTGGGGTGTGGCCCTGAAGAACACCTTGCGCCAGGCGCCAGACGTGATCCTGATCGGTGAAATCCGCG
ACCGTGAAACCATGGACTACGCCATCGCTTTCGCCGAAACCGGGCACTTGTGCCTGGCCACGCTGCATGCCAACAGCTCC
AACCAGGCGCTCGACCGTATCATCAACTTTTTCCCGGAAGAACGCCGGCCGCAACTGCTGATGGACTTGTCGCTCAATCT
TAAAGGGATCGTCTCGCAACGCCTGGTACCGCTAAAAACGGTCAAGGGCCGTGCGGTCGCGGTGGAGATCCTGCTGAATT
CACCTTTGATTTCCGACCTGATCTTCAAGGGCGATGTCCATGAAATCAAGGAAATCATGAAGAAGTCGCGCGAGCTCGGC
ATGCAGACTTTTGACCAGGCCCTGTTCGATTTGTACGAAGACGATAAAATCAGCTATGAAGACGCTTTGCGCAATGCCGA
TTCGGTCAACGACCTGCGGCTTTCCATCAAACTGCGCGGCAAGGACGCAAAAGACCGCGACCTGTCTGCCGGCACTGAAC
ATCTGGGGATCGTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A9KH81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.474

91.534

0.608
  pilU Acinetobacter baylyi ADP1

60.286

92.593

0.558
  pilU Vibrio cholerae strain A1552

57.637

91.799

0.529
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.582

88.624

0.386
  pilT Legionella pneumophila strain Lp02

41.692

87.566

0.365
  pilT Legionella pneumophila strain ERS1305867

41.692

87.566

0.365
  pilT Pseudomonas aeruginosa PAK

41.194

88.624

0.365

Multiple sequence alignment