Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FORC31_RS04485 Genome accession   NZ_CP013190
Coordinates   884605..885585 (+) Length   326 a.a.
NCBI ID   WP_001355637.1    Uniprot ID   A0A8T5WCU8
Organism   Escherichia coli strain FORC_031     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 879605..890585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC31_RS04455 (FORC31_0912) - 879983..880969 (-) 987 WP_000783998.1 TRAP transporter substrate-binding protein -
  FORC31_RS04460 (FORC31_0913) hemW 881282..882418 (-) 1137 WP_000239952.1 radical SAM family heme chaperone HemW -
  FORC31_RS04465 (FORC31_0914) rdgB 882411..883004 (-) 594 WP_001174736.1 XTP/dITP diphosphatase -
  FORC31_RS04470 (FORC31_0915) yggU 883012..883302 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  FORC31_RS04475 (FORC31_0916) yggT 883299..883865 (-) 567 WP_001094830.1 osmotic shock tolerance protein YggT -
  FORC31_RS04480 (FORC31_0917) yggS 883883..884587 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  FORC31_RS04485 (FORC31_0918) pilT 884605..885585 (+) 981 WP_001355637.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FORC31_RS04490 (FORC31_0919) ruvX 885760..886176 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  FORC31_RS04495 (FORC31_0920) yqgE 886176..886739 (-) 564 WP_001355636.1 YqgE/AlgH family protein -
  FORC31_RS04500 (FORC31_0921) gshB 886848..887798 (-) 951 WP_000593266.1 glutathione synthase -
  FORC31_RS04505 (FORC31_0922) rsmE 887811..888542 (-) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FORC31_RS04510 (FORC31_0923) endA 888622..889329 (-) 708 WP_000286500.1 deoxyribonuclease I -
  FORC31_RS04515 (FORC31_0924) yggI 889424..889921 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36002.22 Da        Isoelectric Point: 5.7980

>NTDB_id=160545 FORC31_RS04485 WP_001355637.1 884605..885585(+) (pilT) [Escherichia coli strain FORC_031]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPLILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=160545 FORC31_RS04485 WP_001355637.1 884605..885585(+) (pilT) [Escherichia coli strain FORC_031]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACTGATATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGTAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
TGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTACGTGACAGCGAGACAATCCGTCTGGCGCTTACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTAGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACGCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.063

97.239

0.39
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilB Legionella pneumophila strain ERS1305867

31.152

100

0.365
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment