Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CMT41_RS12660 Genome accession   NZ_CP013145
Coordinates   2953489..2954532 (-) Length   347 a.a.
NCBI ID   WP_057181635.1    Uniprot ID   A0A0S2JHJ4
Organism   Colwellia sp. MT41     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2948489..2959532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CMT41_RS12640 (CMT41_12640) - 2949022..2949861 (-) 840 WP_232299958.1 adenosylcobinamide-GDP ribazoletransferase -
  CMT41_RS12645 (CMT41_12645) cobT 2949858..2951024 (-) 1167 WP_058027464.1 nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase -
  CMT41_RS12650 (CMT41_12650) - 2951259..2951810 (+) 552 WP_057181633.1 hypothetical protein -
  CMT41_RS12655 (CMT41_12655) pilU 2952261..2953388 (-) 1128 WP_057181634.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CMT41_RS12660 (CMT41_12660) pilT 2953489..2954532 (-) 1044 WP_057181635.1 type IV pilus twitching motility protein PilT Machinery gene
  CMT41_RS12665 (CMT41_12665) - 2954651..2955370 (+) 720 WP_057181636.1 YggS family pyridoxal phosphate-dependent enzyme -
  CMT41_RS12670 (CMT41_12670) proC 2955387..2956208 (+) 822 WP_058027465.1 pyrroline-5-carboxylate reductase -
  CMT41_RS12675 (CMT41_12675) - 2956267..2956812 (+) 546 WP_057181638.1 YggT family protein -
  CMT41_RS12680 (CMT41_12680) - 2957185..2957625 (+) 441 WP_057181639.1 DUF4426 domain-containing protein -
  CMT41_RS12685 (CMT41_12685) rdgB 2957775..2958389 (+) 615 WP_232300073.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38523.30 Da        Isoelectric Point: 6.5034

>NTDB_id=160305 CMT41_RS12660 WP_057181635.1 2953489..2954532(-) (pilT) [Colwellia sp. MT41]
MDITELLAFSVQHNASDLHLSTGTPPSIRVDGDIRKLNIPAFDAKDVNALVYDIMNDRQRKEYEEKLEVDFSFEVPSLAR
FRVNAFHQHRGPAAVFRTIPSKILSLDDLGCPEIFREISDTPRGLVLVTGPTGSGKSTTLAAMVDYINSTKHDHILTIED
PIEFVHENKKCLINQREVHRDTLSFSAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKAMVRSMLSESLRAVISQTLIKKVGGGRVAAHEIMIGVPAIRNLIREDKIAQMYSAIQTGMQHGMQTMDQCL
QGLVNRGIITKQAAMEKAVDKNQFIGL

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=160305 CMT41_RS12660 WP_057181635.1 2953489..2954532(-) (pilT) [Colwellia sp. MT41]
ATGGATATTACCGAACTACTTGCATTTAGCGTACAGCATAATGCATCAGATTTACATTTATCAACGGGGACACCGCCGAG
TATTCGAGTGGATGGCGATATACGTAAACTCAATATCCCAGCTTTTGATGCTAAAGATGTCAATGCCTTAGTGTATGACA
TTATGAATGATCGTCAGCGTAAAGAATATGAAGAAAAGTTAGAAGTCGATTTCTCTTTTGAGGTGCCCAGTTTAGCGCGT
TTTAGGGTTAATGCCTTCCATCAACATCGCGGTCCAGCCGCTGTTTTTCGTACCATTCCTAGTAAAATCCTCTCCCTTGA
TGACTTAGGTTGTCCGGAAATATTTCGTGAAATTTCCGATACCCCACGCGGCTTAGTGTTGGTTACTGGACCTACCGGCT
CAGGTAAGTCAACCACCTTAGCGGCCATGGTTGATTATATCAACAGTACTAAACATGATCATATTCTCACCATAGAAGAT
CCTATTGAATTTGTTCATGAGAATAAAAAATGCCTAATAAACCAAAGGGAAGTACATCGTGATACTTTAAGCTTTTCAGC
GGCATTGCGCTCAGCCTTACGTGAAGACCCCGATGTAATTCTTGTTGGTGAAATGCGTGATTTAGAAACTATTCGTTTAG
CCATGACAGCAGCAGAAACGGGTCATCTGGTCTTTGGTACACTGCATACCACTTCAGCACCGAAAACCATTGACCGTATT
ATTGATGTTTTTCCCGGAGAAGAAAAAGCTATGGTGCGCTCTATGCTGTCTGAGTCACTGCGTGCGGTTATTTCACAAAC
CTTGATTAAAAAGGTGGGTGGTGGTCGTGTTGCCGCCCATGAAATTATGATTGGTGTACCCGCTATTCGTAACCTTATTC
GCGAGGATAAAATAGCGCAAATGTATTCTGCTATCCAAACGGGTATGCAACACGGTATGCAAACCATGGATCAATGTCTA
CAAGGCCTAGTCAATCGCGGCATCATTACTAAACAAGCCGCCATGGAAAAAGCGGTTGATAAAAATCAATTCATTGGTTT
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0S2JHJ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.326

99.135

0.767
  pilT Pseudomonas aeruginosa PAK

77.811

97.406

0.758
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.872

99.135

0.752
  pilT Legionella pneumophila strain Lp02

73.837

99.135

0.732
  pilT Legionella pneumophila strain ERS1305867

73.837

99.135

0.732
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.118

0.706
  pilT Vibrio cholerae strain A1552

72.7

97.118

0.706
  pilT Neisseria gonorrhoeae MS11

68.142

97.695

0.666
  pilT Neisseria meningitidis 8013

68.142

97.695

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.623

99.424

0.533
  pilU Vibrio cholerae strain A1552

41.983

98.847

0.415
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.233

98.847

0.398

Multiple sequence alignment