Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CMT41_RS12655 Genome accession   NZ_CP013145
Coordinates   2952261..2953388 (-) Length   375 a.a.
NCBI ID   WP_057181634.1    Uniprot ID   A0A0S2JHX3
Organism   Colwellia sp. MT41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2947261..2958388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CMT41_RS12630 (CMT41_12630) - 2947503..2948423 (-) 921 WP_232299957.1 histidine phosphatase family protein -
  CMT41_RS12635 (CMT41_12635) cobU 2948459..2949025 (-) 567 WP_058027463.1 bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase -
  CMT41_RS12640 (CMT41_12640) - 2949022..2949861 (-) 840 WP_232299958.1 adenosylcobinamide-GDP ribazoletransferase -
  CMT41_RS12645 (CMT41_12645) cobT 2949858..2951024 (-) 1167 WP_058027464.1 nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase -
  CMT41_RS12650 (CMT41_12650) - 2951259..2951810 (+) 552 WP_057181633.1 hypothetical protein -
  CMT41_RS12655 (CMT41_12655) pilU 2952261..2953388 (-) 1128 WP_057181634.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CMT41_RS12660 (CMT41_12660) pilT 2953489..2954532 (-) 1044 WP_057181635.1 type IV pilus twitching motility protein PilT Machinery gene
  CMT41_RS12665 (CMT41_12665) - 2954651..2955370 (+) 720 WP_057181636.1 YggS family pyridoxal phosphate-dependent enzyme -
  CMT41_RS12670 (CMT41_12670) proC 2955387..2956208 (+) 822 WP_058027465.1 pyrroline-5-carboxylate reductase -
  CMT41_RS12675 (CMT41_12675) - 2956267..2956812 (+) 546 WP_057181638.1 YggT family protein -
  CMT41_RS12680 (CMT41_12680) - 2957185..2957625 (+) 441 WP_057181639.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41500.54 Da        Isoelectric Point: 5.5301

>NTDB_id=160304 CMT41_RS12655 WP_057181634.1 2952261..2953388(-) (pilU) [Colwellia sp. MT41]
MKLHQFLERMVKQDASDMFVTAKMPVSAKINGELQPIDDVVLTAEESLELVHAAMNEKQQQQFHTEKECNFAIEIDDIGR
FRVSAFWQRDMAGMVIRRIVTVIPDVDDLGLPPILKDVIMSKRGLVLFVGGTGTGKSTSMASLIGYRNRNSCGHILTIED
PVEFVHEHGKSMITQREVGLDTVSFDAALQSSLRQAPDVILIGEIRNQEVMEHALSFAETGHLCIATLHANNANQAIDRI
MHLVPADQHGKLLFDLALNLRAIIAQQLVPTKDGHSRVAVIEVLLNSPYVSELIKKGDIGSIKEVMEKSNELGMKTFDQA
LFSHYQQGMITYADAIHHADSPNDLRLMIKLRNNEQGGTGGLSGVTLDGFEPTDN

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=160304 CMT41_RS12655 WP_057181634.1 2952261..2953388(-) (pilU) [Colwellia sp. MT41]
ATGAAACTGCATCAATTTTTAGAAAGAATGGTAAAACAAGACGCTTCTGATATGTTTGTTACCGCCAAAATGCCAGTGAG
TGCAAAAATAAATGGTGAATTACAACCGATAGATGATGTGGTTTTAACTGCTGAAGAGTCTTTAGAACTCGTTCATGCTG
CCATGAATGAAAAGCAGCAGCAGCAATTTCATACCGAAAAAGAATGTAACTTTGCCATAGAAATTGATGATATTGGTCGT
TTTCGGGTCTCAGCTTTTTGGCAGCGGGATATGGCTGGTATGGTTATTCGTCGAATAGTTACCGTAATCCCCGATGTTGA
TGACTTAGGTCTACCGCCGATATTGAAAGACGTCATTATGTCTAAACGTGGCTTAGTGTTATTTGTTGGTGGTACGGGTA
CGGGTAAATCAACCTCGATGGCATCACTTATCGGTTATCGTAACCGTAACTCTTGTGGCCATATTTTAACCATTGAAGAT
CCGGTGGAATTTGTCCACGAACATGGTAAAAGTATGATCACTCAACGCGAAGTTGGCTTAGATACCGTGTCATTTGATGC
CGCACTGCAAAGTTCATTACGACAGGCGCCCGATGTTATCTTAATTGGTGAAATTCGTAACCAAGAGGTGATGGAGCATG
CGCTTAGTTTTGCTGAGACCGGGCACTTATGTATCGCTACCTTACATGCTAACAACGCCAACCAAGCCATTGATCGGATT
ATGCATCTAGTACCTGCGGATCAACACGGTAAACTATTATTTGATTTGGCCTTAAACCTGCGTGCTATTATTGCGCAACA
ACTAGTACCGACTAAAGATGGTCATAGCCGAGTTGCCGTCATTGAGGTCTTACTGAACTCTCCTTATGTAAGTGAACTTA
TCAAAAAAGGTGACATTGGTAGTATTAAAGAAGTTATGGAAAAGTCCAATGAGTTGGGTATGAAAACGTTCGATCAGGCG
CTATTTAGCCATTATCAACAGGGGATGATTACGTATGCGGATGCCATACATCATGCTGATTCACCGAATGATTTACGGTT
AATGATCAAATTACGTAATAATGAGCAAGGTGGCACCGGTGGTTTATCTGGCGTAACGCTTGATGGTTTTGAGCCGACAG
ATAACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0S2JHX3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.824

95.2

0.56
  pilU Acinetobacter baylyi ADP1

59.091

93.867

0.555
  pilU Vibrio cholerae strain A1552

52.717

98.133

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.165

93.6

0.395

Multiple sequence alignment