Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AOC03_RS07950 Genome accession   NZ_CP012678
Coordinates   1842975..1844030 (-) Length   351 a.a.
NCBI ID   WP_062534879.1    Uniprot ID   -
Organism   Psychrobacter urativorans strain R310.10B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1837975..1849030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOC03_RS07925 (AOC03_07910) - 1838871..1840022 (+) 1152 WP_062534871.1 hypothetical protein -
  AOC03_RS07930 (AOC03_07915) - 1840010..1840348 (+) 339 WP_227514214.1 RnfH family protein -
  AOC03_RS07935 (AOC03_07920) - 1840410..1840832 (-) 423 WP_062536612.1 outer membrane protein assembly factor BamE -
  AOC03_RS07940 (AOC03_07925) fur 1841157..1841591 (+) 435 WP_062534875.1 ferric iron uptake transcriptional regulator -
  AOC03_RS07945 (AOC03_07930) pilU 1841719..1842840 (-) 1122 WP_062534877.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AOC03_RS07950 (AOC03_07935) pilT 1842975..1844030 (-) 1056 WP_062534879.1 type IV pilus twitching motility protein PilT Machinery gene
  AOC03_RS07955 (AOC03_07940) - 1844405..1845106 (+) 702 WP_062534881.1 YggS family pyridoxal phosphate-dependent enzyme -
  AOC03_RS07960 (AOC03_07945) - 1845123..1846397 (-) 1275 WP_062534883.1 hemolysin family protein -
  AOC03_RS07965 (AOC03_07950) - 1846611..1848302 (-) 1692 WP_062534885.1 NAD+ synthase -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38914.84 Da        Isoelectric Point: 7.0898

>NTDB_id=156276 AOC03_RS07950 WP_062534879.1 1842975..1844030(-) (pilT) [Psychrobacter urativorans strain R310.10B]
MTGKSHLSIEDLLRFTVKHKASDLHISAGMPAMIRVDGEMTRINMPVMTHQVVHQLIYDIMNDKQRADFEEFLETDFSFE
IPGLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGDVFKRVSDFKRGVVLVTGPTGSGKSTTLAAMIDYINETRKEH
ILTIEDPIEFVHESKKSLINQREVHRDTLGFDAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAA
KTIDRVIDVFPAAEKDMIRAMVSESLQAVISQTLLKRQTGGRVAAHEIMLGTPAIRNLIRENKIAQMYSSIQTGAGDGMI
TLDQTLKNLVAKGTISKDVARPYAKQPEAFI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=156276 AOC03_RS07950 WP_062534879.1 1842975..1844030(-) (pilT) [Psychrobacter urativorans strain R310.10B]
ATGACCGGAAAAAGCCACTTAAGTATTGAAGATTTGCTGCGTTTTACGGTTAAGCACAAGGCATCAGATTTACATATATC
GGCTGGAATGCCAGCAATGATTCGGGTCGATGGTGAGATGACGCGCATCAATATGCCAGTGATGACGCATCAGGTGGTGC
ATCAGCTGATTTACGATATTATGAATGATAAGCAACGTGCTGACTTTGAAGAATTTCTTGAGACCGACTTTTCGTTCGAG
ATTCCAGGACTTGCGCGCTTTCGAGTCAATGCTTTTAATCAAAATCGGGGTGCAGGCGCAGTATTTCGTACCATTCCATC
CAAGGTTTTGACCATGGAAGATTTAGGCATGGGCGACGTATTCAAGCGCGTCTCAGATTTCAAGCGTGGCGTCGTGTTGG
TCACGGGTCCTACGGGCTCAGGAAAATCAACGACGCTCGCGGCGATGATTGATTATATTAACGAGACTCGTAAAGAGCAT
ATTTTAACGATTGAAGATCCTATTGAATTTGTGCATGAATCTAAAAAAAGCCTAATCAATCAGCGTGAGGTGCACCGCGA
TACCTTAGGTTTTGATGCCGCATTGCGCTCAGCATTACGTGAGGATCCCGACGTTATCTTAGTCGGTGAGCTACGTGACC
TTGAAACGATTCGTCTGGCACTAACGGCTGCTGAAACAGGGCACTTAGTGTTTGGTACATTGCACACCAGCTCTGCTGCC
AAGACTATTGACCGCGTTATTGACGTGTTCCCTGCGGCTGAAAAAGACATGATTCGCGCGATGGTGTCAGAATCTCTACA
AGCCGTTATCTCACAAACGCTATTAAAAAGACAAACGGGTGGTCGTGTCGCGGCACATGAAATCATGCTAGGAACACCTG
CTATTCGTAACTTAATTCGCGAAAACAAAATTGCGCAAATGTACTCTTCTATCCAGACAGGTGCAGGCGACGGCATGATT
ACCCTTGATCAAACACTCAAAAATCTAGTCGCAAAAGGCACTATCAGTAAAGACGTAGCACGACCTTATGCTAAGCAACC
TGAAGCCTTTATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

79.13

98.291

0.778
  pilT Acinetobacter baumannii D1279779

79.13

98.291

0.778
  pilT Acinetobacter nosocomialis M2

78.841

98.291

0.775
  pilT Pseudomonas stutzeri DSM 10701

78.779

98.006

0.772
  pilT Pseudomonas aeruginosa PAK

78.779

98.006

0.772
  pilT Acinetobacter baylyi ADP1

77.035

98.006

0.755
  pilT Legionella pneumophila strain Lp02

71.802

98.006

0.704
  pilT Legionella pneumophila strain ERS1305867

71.802

98.006

0.704
  pilT Neisseria meningitidis 8013

64.638

98.291

0.635
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.781

93.732

0.635
  pilT Vibrio cholerae strain A1552

67.781

93.732

0.635
  pilT Neisseria gonorrhoeae MS11

64.058

98.291

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.009

98.86

0.504
  pilU Acinetobacter baylyi ADP1

41.108

97.721

0.402
  pilU Pseudomonas stutzeri DSM 10701

40.708

96.581

0.393
  pilU Vibrio cholerae strain A1552

41.796

92.023

0.385

Multiple sequence alignment