Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AOC03_RS07945 Genome accession   NZ_CP012678
Coordinates   1841719..1842840 (-) Length   373 a.a.
NCBI ID   WP_062534877.1    Uniprot ID   A0A0M3V941
Organism   Psychrobacter urativorans strain R310.10B     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1836719..1847840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOC03_RS07915 (AOC03_07900) - 1837172..1837507 (+) 336 WP_062534868.1 c-type cytochrome -
  AOC03_RS07920 (AOC03_07905) - 1837944..1838612 (+) 669 WP_062534869.1 c-type cytochrome -
  AOC03_RS07925 (AOC03_07910) - 1838871..1840022 (+) 1152 WP_062534871.1 hypothetical protein -
  AOC03_RS07930 (AOC03_07915) - 1840010..1840348 (+) 339 WP_227514214.1 RnfH family protein -
  AOC03_RS07935 (AOC03_07920) - 1840410..1840832 (-) 423 WP_062536612.1 outer membrane protein assembly factor BamE -
  AOC03_RS07940 (AOC03_07925) fur 1841157..1841591 (+) 435 WP_062534875.1 ferric iron uptake transcriptional regulator -
  AOC03_RS07945 (AOC03_07930) pilU 1841719..1842840 (-) 1122 WP_062534877.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AOC03_RS07950 (AOC03_07935) pilT 1842975..1844030 (-) 1056 WP_062534879.1 type IV pilus twitching motility protein PilT Machinery gene
  AOC03_RS07955 (AOC03_07940) - 1844405..1845106 (+) 702 WP_062534881.1 YggS family pyridoxal phosphate-dependent enzyme -
  AOC03_RS07960 (AOC03_07945) - 1845123..1846397 (-) 1275 WP_062534883.1 hemolysin family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 42007.04 Da        Isoelectric Point: 6.3715

>NTDB_id=156275 AOC03_RS07945 WP_062534877.1 1841719..1842840(-) (pilU) [Psychrobacter urativorans strain R310.10B]
MDFDKLLQLMIKNNGSDLFITADVAPSMKINGTIRPVGKMPLTPENTMALVKSVMTKSQQHEFEETNECQFAITDNAQQA
RFRVSAFMQRDMAGMILRKIENKIPTVEELRLPPSLKELAMRKRGIILLVGATGTGKSTTLAAMVGYRNQNSHGHIITIE
DPIEFVHQHHGCIITQREVGIDTLTFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFESDRHNQLFMDLSLNLQAVIAQQLIPTPDGKSRRAAIEILLNSQLISDYIRKGEVHEIKDVMTRSRDSGMQTFDQ
ALFDLYENGEITYQDAISHADSPNDLRLKIKLNSKNINQNLDEGADRLSLDIN

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=156275 AOC03_RS07945 WP_062534877.1 1841719..1842840(-) (pilU) [Psychrobacter urativorans strain R310.10B]
ATGGATTTTGATAAACTGCTGCAACTGATGATCAAAAATAATGGTTCTGACTTATTTATTACCGCTGATGTAGCACCGTC
TATGAAAATAAATGGCACTATTCGTCCGGTTGGTAAAATGCCATTAACGCCTGAAAATACCATGGCGTTGGTCAAAAGTG
TGATGACAAAAAGTCAGCAACACGAGTTTGAAGAAACGAACGAGTGCCAATTTGCCATCACCGATAATGCCCAACAAGCG
CGCTTTCGGGTCAGCGCCTTTATGCAGCGTGATATGGCAGGGATGATTTTACGGAAAATCGAAAATAAGATTCCGACGGT
TGAAGAGTTACGCCTACCGCCCTCTTTAAAAGAGCTGGCGATGCGCAAGCGTGGCATTATTTTATTAGTGGGTGCTACAG
GTACAGGTAAATCGACGACCCTTGCGGCAATGGTTGGCTATCGCAATCAGAACTCGCATGGTCATATCATCACCATCGAA
GACCCTATTGAATTCGTGCATCAACATCATGGCTGTATCATCACCCAACGCGAGGTTGGCATTGATACTTTAACCTTTGA
GGCTGGTCTAAAAAACACCTTGCGCCAAGCACCTGATGTCATCTTAATTGGTGAGATTCGTAACCGCGAGGTTATGAGTT
ATGCCATTCAATATGCTGAAACCGGACATTTGGTGTTTGCCACTTTGCACGCCAATAATGCCAACCAAGCCATTGACCGC
ATCATTCACTTTTTTGAGAGCGATCGTCATAATCAGCTGTTTATGGATTTATCGCTGAACTTACAAGCGGTTATCGCACA
ACAGCTTATCCCAACCCCTGACGGTAAAAGTCGTCGGGCTGCTATCGAGATTTTATTAAACTCGCAGCTCATCAGTGACT
ATATCCGTAAAGGTGAGGTGCATGAAATCAAAGATGTCATGACGCGCTCACGTGATAGCGGTATGCAAACCTTTGATCAG
GCGCTGTTCGATTTATATGAAAATGGTGAAATTACCTACCAAGATGCTATCAGTCATGCCGACTCACCAAATGACTTGCG
CCTCAAAATTAAATTGAATAGCAAAAATATCAATCAAAATCTTGATGAAGGCGCCGATCGCTTGTCATTAGACATTAACT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(41-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0M3V941

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

67.403

97.051

0.654
  pilU Pseudomonas stutzeri DSM 10701

64.879

100

0.649
  pilU Vibrio cholerae strain A1552

53.846

94.102

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.908

92.761

0.389
  pilT Acinetobacter nosocomialis M2

41.108

91.957

0.378
  pilT Acinetobacter baumannii D1279779

41.108

91.957

0.378
  pilT Acinetobacter baumannii strain A118

41.108

91.957

0.378
  pilT Legionella pneumophila strain ERS1305867

41.667

90.08

0.375
  pilT Legionella pneumophila strain Lp02

41.667

90.08

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.124

90.617

0.373
  pilT Vibrio cholerae strain A1552

41.124

90.617

0.373
  pilT Pseudomonas aeruginosa PAK

39.884

92.761

0.37
  pilT Acinetobacter baylyi ADP1

39.65

91.957

0.365

Multiple sequence alignment