Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AMQ28_RS11565 Genome accession   NZ_CP012608
Coordinates   2423449..2424486 (-) Length   345 a.a.
NCBI ID   WP_053579733.1    Uniprot ID   A0A0M4GVS7
Organism   Acinetobacter sp. TTH0-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2418449..2429486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMQ28_RS11535 (AMQ28_11540) - 2419258..2419707 (+) 450 WP_053579727.1 bacteriohemerythrin -
  AMQ28_RS11540 (AMQ28_11545) - 2419848..2420930 (+) 1083 WP_053579728.1 hypothetical protein -
  AMQ28_RS11545 (AMQ28_11550) - 2420930..2421253 (+) 324 WP_053579729.1 RnfH family protein -
  AMQ28_RS11550 (AMQ28_11555) - 2421298..2421696 (-) 399 WP_053579730.1 outer membrane protein assembly factor BamE -
  AMQ28_RS11555 (AMQ28_11560) fur 2421809..2422246 (+) 438 WP_053579731.1 ferric iron uptake transcriptional regulator -
  AMQ28_RS11560 (AMQ28_11565) pilU 2422302..2423423 (-) 1122 WP_053579732.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AMQ28_RS11565 (AMQ28_11570) pilT 2423449..2424486 (-) 1038 WP_053579733.1 type IV pilus twitching motility protein PilT Machinery gene
  AMQ28_RS11570 (AMQ28_11575) - 2424611..2425297 (+) 687 WP_053579734.1 YggS family pyridoxal phosphate-dependent enzyme -
  AMQ28_RS11575 (AMQ28_11580) - 2425647..2429255 (-) 3609 WP_053579735.1 AAA family ATPase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38263.00 Da        Isoelectric Point: 6.7273

>NTDB_id=155774 AMQ28_RS11565 WP_053579733.1 2423449..2424486(-) (pilT) [Acinetobacter sp. TTH0-4]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDFEEKLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTIEDLGMGQIFKDICNFPRGIVLVTGPTGSGKSTTLAAMVDYINDHRYDHILTVED
PIEFVHESKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVAKGMISPQTARTMAKQPEAFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=155774 AMQ28_RS11565 WP_053579733.1 2423449..2424486(-) (pilT) [Acinetobacter sp. TTH0-4]
ATGGACATTACAGAGTTACTGGCCTTTTCGGCTAAAAATGGAGCATCTGACTTACATTTATCCGCGGGCATGCCCCCCCT
CATTCGAGTCGACGGTGAAGTGCGCCGTATTAATTTACCAGCTTTGGAACATAAAGAAGTACACAAATTGGTGTATGACA
TTATGAATGATAAACAACGCCGTGATTTTGAAGAAAAATTAGAAACTGACTTTTCTTTTGAAGTTCCCGGTGTAGCACGT
TTTCGTGTCAATGCTTTTAACCAAAACCGTGGTGCAGGTGCAGTATTCCGTACCATTCCATCAAAAGTTTTAACCATTGA
AGACTTGGGCATGGGGCAAATCTTTAAAGATATTTGTAATTTCCCACGTGGTATTGTTTTGGTTACAGGGCCGACAGGTT
CGGGGAAATCGACCACACTCGCAGCAATGGTCGACTATATTAATGATCATCGTTATGACCATATTTTAACTGTAGAAGAT
CCGATTGAATTTGTACACGAATCAAAAAAATGTCTGGTCAATCAGCGTGAAGTCCATCGTGATACACATGGTTTTAACGA
AGCATTACGCTCAGCTTTGCGTGAAGATCCTGACATTATTTTGGTCGGTGAGATGCGTGATCTTGAAACCATCCGTTTGG
CTTTAACGGCTGCTGAAACAGGCCACTTGGTCTTTGGCACGCTACATACTACTTCTGCGGCAAAAACCATTGACCGTGTG
ATTGACGTCTTCCCTGCTGAAGAAAAAGATATGGTTCGTGCCATGTTATCTGAATCCTTACAAGCCGTGATTTCTCAAAC
TTTACTGAAGAAAAATGGGGGTGGACGTGTGGCTGCACATGAAATCATGATTGGAATTCCAGCGATTCGTAACTTAATTC
GTGAAAATAAAGTGGCTCAAATGTATTCTGCCATTCAAACAGGTGCCAACTACGGCATGACCACGCTGGATCAAAGTTTA
AAAACGTTAGTGGCTAAAGGCATGATTAGTCCCCAAACAGCACGGACAATGGCAAAACAACCAGAAGCCTTCTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0M4GVS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

93.913

100

0.939
  pilT Acinetobacter baumannii D1279779

93.913

100

0.939
  pilT Acinetobacter nosocomialis M2

93.623

100

0.936
  pilT Acinetobacter baylyi ADP1

86.919

99.71

0.867
  pilT Pseudomonas aeruginosa PAK

84.884

99.71

0.846
  pilT Pseudomonas stutzeri DSM 10701

84.593

99.71

0.843
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.345

97.391

0.675
  pilT Vibrio cholerae strain A1552

69.345

97.391

0.675
  pilT Neisseria meningitidis 8013

66.667

100

0.667
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.664
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

42.687

97.101

0.414
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

39.65

99.42

0.394

Multiple sequence alignment