Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AMQ28_RS11560 Genome accession   NZ_CP012608
Coordinates   2422302..2423423 (-) Length   373 a.a.
NCBI ID   WP_053579732.1    Uniprot ID   -
Organism   Acinetobacter sp. TTH0-4     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2417302..2428423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMQ28_RS11530 (AMQ28_11535) - 2418072..2418920 (+) 849 WP_053579726.1 class II glutamine amidotransferase -
  AMQ28_RS11535 (AMQ28_11540) - 2419258..2419707 (+) 450 WP_053579727.1 bacteriohemerythrin -
  AMQ28_RS11540 (AMQ28_11545) - 2419848..2420930 (+) 1083 WP_053579728.1 hypothetical protein -
  AMQ28_RS11545 (AMQ28_11550) - 2420930..2421253 (+) 324 WP_053579729.1 RnfH family protein -
  AMQ28_RS11550 (AMQ28_11555) - 2421298..2421696 (-) 399 WP_053579730.1 outer membrane protein assembly factor BamE -
  AMQ28_RS11555 (AMQ28_11560) fur 2421809..2422246 (+) 438 WP_053579731.1 ferric iron uptake transcriptional regulator -
  AMQ28_RS11560 (AMQ28_11565) pilU 2422302..2423423 (-) 1122 WP_053579732.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AMQ28_RS11565 (AMQ28_11570) pilT 2423449..2424486 (-) 1038 WP_053579733.1 type IV pilus twitching motility protein PilT Machinery gene
  AMQ28_RS11570 (AMQ28_11575) - 2424611..2425297 (+) 687 WP_053579734.1 YggS family pyridoxal phosphate-dependent enzyme -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 42015.33 Da        Isoelectric Point: 6.7815

>NTDB_id=155773 AMQ28_RS11560 WP_053579732.1 2422302..2423423(-) (pilU) [Acinetobacter sp. TTH0-4]
MDFNDLLNLMIQKQASDLFLTADVEPSMKINGQILPIAKTKLTGEIVGQLLNSIMSDKQRKEFAETRECNFAINNRDKTA
RFRVSAFQQRDHPGMVLRRIETVIPTMDELKLPPILKELAMTKRGIIIFVGATGTGKSTSLASIIGYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNLRAMVAQQLIPTIDGNSRRAAIEILINSPLISDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGADRLMHAGTNITFDGQS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=155773 AMQ28_RS11560 WP_053579732.1 2422302..2423423(-) (pilU) [Acinetobacter sp. TTH0-4]
ATGGATTTTAACGACTTACTCAATTTGATGATTCAAAAACAAGCATCAGACTTATTTCTCACTGCTGATGTTGAACCTTC
AATGAAAATTAATGGTCAGATTCTTCCGATTGCAAAAACAAAGCTGACAGGAGAAATAGTGGGTCAGCTTTTAAATTCAA
TCATGAGTGACAAGCAACGTAAAGAATTTGCTGAAACTCGTGAATGTAACTTTGCCATTAATAATCGTGATAAAACTGCG
CGTTTTCGTGTCAGTGCTTTTCAACAGCGTGATCATCCTGGCATGGTATTACGTCGTATCGAAACGGTTATTCCAACCAT
GGATGAGCTCAAGTTACCGCCTATTTTAAAAGAACTGGCTATGACTAAACGCGGTATTATCATCTTTGTAGGAGCAACGG
GAACAGGTAAATCAACATCTTTGGCTTCTATTATTGGTTATCGTAACCATAATTCCAAAGGTCATATCATTACCATTGAA
GATCCGATTGAATTTATTCATGAACATGCGGGCTGTATTATTACCCAGCGTGAAGTCGGTATTGATACAGACTCTTTTGA
AATTGCGTTAAAGAATACCTTACGTCAAGCACCCGATGTAATTTTGATTGGGGAAATTCGTTCACGTGAAACCATGGACT
ATGCCATTGCCTTTGCTGAAACGGGTCACTTAGTATTTGCAACGCTGCATGCCAACAACGCCAACCAAGCCATTGATCGT
ATTATTCATTTCTTTGAAGCTGACCGTCATAACCAACTCTTTATGGACTTGTCGCTTAATTTAAGAGCCATGGTGGCTCA
GCAGTTGATTCCGACCATAGATGGCAATTCACGTCGAGCGGCCATCGAAATTCTGATTAACTCTCCATTAATTTCAGACC
TGATCCGCAAAGGAGATGTGCATGAAATTAAGGATTTGATGAAGCGCTCACGCGAACTGGGTATGCAAACTTTTGACCAA
GCTTTATTTGATTTATATAAGGCCAAGCAAATTACCTACAAAGATGCACTGAAACATGCGGACTCACCAAACGATTTGCG
TTTACAAATCAAACTCTCAGAAGAAGGGGCTGATCGTTTAATGCATGCTGGCACCAATATTACCTTTGATGGTCAATCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.397

100

0.764
  pilU Pseudomonas stutzeri DSM 10701

70.141

95.174

0.668
  pilU Vibrio cholerae strain A1552

53.243

99.196

0.528
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.353

92.761

0.402
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.886

94.102

0.375
  pilT Vibrio cholerae strain A1552

39.886

94.102

0.375
  pilT Pseudomonas aeruginosa PAK

40.462

92.761

0.375
  pilT Acinetobacter baumannii strain A118

40.525

91.957

0.373
  pilT Acinetobacter baumannii D1279779

40.525

91.957

0.373
  pilT Acinetobacter baylyi ADP1

40.525

91.957

0.373
  pilT Acinetobacter nosocomialis M2

40.525

91.957

0.373
  pilT Legionella pneumophila strain Lp02

41.846

87.131

0.365
  pilT Legionella pneumophila strain ERS1305867

41.846

87.131

0.365
  pilT Neisseria meningitidis 8013

39.306

92.761

0.365
  pilT Neisseria gonorrhoeae MS11

39.306

92.761

0.365
  pilT Pseudomonas stutzeri DSM 10701

39.017

92.761

0.362

Multiple sequence alignment