Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AK825_RS01715 Genome accession   NZ_CP012533
Coordinates   398541..399596 (-) Length   351 a.a.
NCBI ID   WP_068034206.1    Uniprot ID   -
Organism   Psychrobacter sp. P11G5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 393541..404596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AK825_RS01685 (AK825_01685) - 393727..394395 (+) 669 WP_068034196.1 c-type cytochrome -
  AK825_RS01690 (AK825_01690) - 394545..395687 (+) 1143 WP_068034198.1 hypothetical protein -
  AK825_RS01695 (AK825_01695) - 395675..396028 (+) 354 WP_227514099.1 RnfH family protein -
  AK825_RS01700 (AK825_01700) bamE 396039..396473 (-) 435 WP_082787136.1 outer membrane protein assembly factor BamE -
  AK825_RS01705 (AK825_01705) fur 396797..397231 (+) 435 WP_068034202.1 ferric iron uptake transcriptional regulator -
  AK825_RS01710 (AK825_01710) pilU 397334..398455 (-) 1122 WP_068034204.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AK825_RS01715 (AK825_01715) pilT 398541..399596 (-) 1056 WP_068034206.1 type IV pilus twitching motility protein PilT Machinery gene
  AK825_RS01720 (AK825_01720) - 399945..400688 (+) 744 WP_068038889.1 YggS family pyridoxal phosphate-dependent enzyme -
  AK825_RS01725 (AK825_01725) - 400766..402040 (-) 1275 WP_068034208.1 hemolysin family protein -
  AK825_RS01730 (AK825_01730) - 402276..403967 (-) 1692 WP_068034210.1 NAD+ synthase -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38878.67 Da        Isoelectric Point: 6.6706

>NTDB_id=155009 AK825_RS01715 WP_068034206.1 398541..399596(-) (pilT) [Psychrobacter sp. P11G5]
MAEKGNLSIEDLLRFTVKHKASDLHLSAGMPAVIRADGEITRINMPVMSHQAVHQLIYDIMNDKQRADFEEFFETDFSFE
IPNLARFRVNAFNQNRGAGAVFRTIPSEVLTLEDLGMGEVFKRVSNFKRGVVLVTGPTGSGKSTTLAAMIDYINDTRKEH
ILTIEDPIEFVHQSKKSLINQREVHRDTLGFEPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAA
KTIDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLPKQTGGRVAAHEIMLGTPAIRNLIRENKIAQMYSAIQTGAGDGMI
TLDQTLKNLVTKGTISKEVARPYAKQPEAFI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=155009 AK825_RS01715 WP_068034206.1 398541..399596(-) (pilT) [Psychrobacter sp. P11G5]
ATGGCTGAAAAAGGCAATTTAAGCATCGAAGATCTGTTGCGATTCACTGTTAAACATAAAGCGTCAGATTTACATCTCTC
AGCTGGAATGCCGGCCGTTATTCGAGCCGATGGTGAGATTACCCGTATTAACATGCCAGTGATGAGTCATCAAGCAGTAC
ATCAGTTAATTTATGACATCATGAATGATAAACAGCGTGCAGATTTTGAGGAATTCTTTGAGACGGATTTTTCTTTTGAG
ATTCCAAATCTAGCTCGCTTTCGGGTAAACGCTTTTAACCAAAATCGCGGTGCAGGTGCTGTATTTCGTACTATTCCATC
AGAAGTTCTAACCTTAGAAGATCTAGGCATGGGAGAGGTATTTAAGCGTGTATCGAACTTCAAACGCGGTGTGGTTCTTG
TCACTGGTCCTACTGGTTCTGGTAAATCTACGACACTTGCTGCGATGATCGACTATATCAATGATACGCGTAAAGAACAT
ATTTTGACCATTGAAGATCCTATCGAATTTGTCCATCAATCCAAAAAAAGCCTTATTAACCAGCGTGAAGTTCACCGTGA
TACCTTAGGTTTTGAGCCTGCATTGCGCTCAGCGCTACGTGAAGATCCAGATGTTATCTTGGTTGGTGAGCTACGTGACC
TTGAAACTATTCGTTTGGCCTTAACCGCTGCTGAAACTGGTCACTTAGTATTTGGCACCTTGCACACCAGCTCCGCTGCC
AAGACGATTGACCGTGTGATTGATGTTTTTCCTGCTGCTGAAAAAGACATGATTCGCGCAATGTTATCTGAATCTCTACA
GGCCGTTATTTCACAGACGTTATTACCAAAACAAACCGGCGGACGTGTTGCTGCTCACGAAATTATGTTAGGTACACCAG
CTATTCGTAACTTAATACGCGAAAACAAAATTGCCCAGATGTATTCCGCTATCCAGACTGGTGCTGGTGACGGCATGATT
ACCCTCGATCAAACGCTCAAAAATTTAGTGACTAAAGGTACTATTAGTAAAGAGGTTGCCCGACCTTATGCTAAGCAGCC
TGAAGCGTTTATATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

78.261

98.291

0.769
  pilT Acinetobacter baumannii strain A118

78.261

98.291

0.769
  pilT Acinetobacter nosocomialis M2

78.261

98.291

0.769
  pilT Pseudomonas aeruginosa PAK

77.616

98.006

0.761
  pilT Pseudomonas stutzeri DSM 10701

76.453

98.006

0.749
  pilT Acinetobacter baylyi ADP1

76.163

98.006

0.746
  pilT Legionella pneumophila strain Lp02

71.512

98.006

0.701
  pilT Legionella pneumophila strain ERS1305867

71.512

98.006

0.701
  pilT Neisseria meningitidis 8013

64.928

98.291

0.638
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.169

94.587

0.635
  pilT Vibrio cholerae strain A1552

67.169

94.587

0.635
  pilT Neisseria gonorrhoeae MS11

64.348

98.291

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.445

98.575

0.507
  pilU Acinetobacter baylyi ADP1

41.399

97.721

0.405
  pilU Pseudomonas stutzeri DSM 10701

41.593

96.581

0.402
  pilU Vibrio cholerae strain A1552

42.105

92.023

0.387

Multiple sequence alignment