Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AFK25_RS11000 Genome accession   NZ_CP012152
Coordinates   2086312..2087346 (-) Length   344 a.a.
NCBI ID   WP_035065514.1    Uniprot ID   A0AAW7TF70
Organism   Anoxybacillus gonensis strain G2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2081312..2092346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AFK25_RS10970 (AFK25_10990) pilO 2081529..2082155 (-) 627 WP_035065532.1 type 4a pilus biogenesis protein PilO -
  AFK25_RS10975 (AFK25_10995) - 2082152..2082733 (-) 582 WP_035065529.1 PilN domain-containing protein -
  AFK25_RS10980 (AFK25_11000) pilM 2082717..2083685 (-) 969 WP_035065526.1 type IV pilus biogenesis protein PilM -
  AFK25_RS10985 (AFK25_11005) comC 2083698..2084447 (-) 750 WP_035065523.1 prepilin peptidase Machinery gene
  AFK25_RS10990 (AFK25_11010) - 2084504..2085019 (-) 516 WP_035065520.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AFK25_RS10995 (AFK25_11015) - 2085095..2086306 (-) 1212 WP_035065517.1 type II secretion system F family protein -
  AFK25_RS11000 (AFK25_11020) pilT 2086312..2087346 (-) 1035 WP_035065514.1 type IV pilus twitching motility protein PilT Machinery gene
  AFK25_RS11005 (AFK25_11025) - 2087357..2089018 (-) 1662 WP_035065511.1 GspE/PulE family protein -
  AFK25_RS11010 (AFK25_11030) - 2089015..2090256 (-) 1242 WP_035065508.1 VanW family protein -
  AFK25_RS11015 (AFK25_11035) - 2090270..2091016 (-) 747 WP_035065505.1 PRC-barrel domain-containing protein -
  AFK25_RS11020 (AFK25_11040) - 2091013..2092209 (-) 1197 WP_035065502.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38187.17 Da        Isoelectric Point: 7.5478

>NTDB_id=151924 AFK25_RS11000 WP_035065514.1 2086312..2087346(-) (pilT) [Anoxybacillus gonensis strain G2]
MRQKVDAMLRAAFELKASDIHLTVGVPPIFRVNGDLKRYGQDVLSPTDTEQMARAIVPEHMWTRFETDGELDLSYSLSGV
SRFRVNVFKQRGCISLAIRIVPTKIPTLEELQLPDVLKKMVTKPQGLILVTGPTGSGKSTTLAAMIDYMNKTMRKHIITL
EDPIEYVHKHGGCIIDQREIGTDTNNFANGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVFGTLHTSSAPATID
RIIDVFQPEQQTQIRIQLATVLVAIISQRLFPRAQQNGRIAATEILINNAAIANLIRNGKIHQIPSIMQTNRALGMHTLE
ANIKELVQQGFIAKESVEPYLQEG

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=151924 AFK25_RS11000 WP_035065514.1 2086312..2087346(-) (pilT) [Anoxybacillus gonensis strain G2]
ATGAGACAAAAAGTAGATGCGATGTTGCGAGCTGCATTTGAGTTAAAAGCATCTGATATTCATTTAACGGTCGGCGTTCC
CCCGATTTTTCGCGTGAACGGAGATTTAAAACGATACGGTCAAGATGTGTTATCGCCAACCGATACAGAACAAATGGCAA
GAGCGATCGTTCCAGAGCATATGTGGACGCGCTTTGAAACAGATGGCGAGTTAGATTTGTCGTATAGCCTTTCCGGTGTT
TCACGCTTCCGTGTGAATGTGTTTAAACAACGTGGATGCATATCGCTTGCCATTCGTATCGTACCGACAAAAATTCCGAC
GCTTGAAGAATTGCAATTGCCAGATGTATTAAAAAAAATGGTAACAAAACCACAAGGGCTTATTCTTGTTACCGGACCGA
CAGGAAGCGGAAAATCGACGACGTTAGCTGCGATGATCGATTATATGAATAAAACGATGCGCAAACATATTATTACGCTT
GAAGATCCAATTGAATACGTGCATAAGCATGGAGGGTGTATTATTGACCAGCGCGAGATCGGGACGGATACAAACAACTT
CGCAAATGGACTTCGAGCGGCGCTTCGGCAAGATCCAGACGTCATTTTAGTTGGAGAGATGCGCGATCTAGAAACGATTC
AAACAGCCATTACCGCCGCGGAAACAGGGCATCTTGTATTCGGGACGCTTCATACGTCCAGCGCACCGGCGACAATTGAT
CGTATCATTGATGTATTTCAACCTGAGCAACAAACACAAATTCGCATTCAATTGGCAACTGTGTTAGTTGCGATTATTTC
GCAACGATTATTTCCACGCGCTCAACAAAACGGACGCATTGCAGCGACAGAAATATTAATTAACAATGCGGCCATTGCAA
ATTTAATTCGCAACGGAAAAATACATCAAATTCCGAGCATTATGCAAACGAACCGCGCGCTCGGTATGCATACGCTTGAA
GCGAACATTAAAGAACTCGTCCAACAAGGATTCATTGCAAAAGAATCGGTTGAGCCGTATTTGCAGGAAGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.923

94.477

0.5
  pilT Vibrio cholerae strain A1552

49.405

97.674

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.405

97.674

0.483
  pilT Pseudomonas aeruginosa PAK

50.153

95.058

0.477
  pilT Pseudomonas stutzeri DSM 10701

49.24

95.64

0.471
  pilT Acinetobacter baumannii D1279779

48.93

95.058

0.465
  pilT Acinetobacter nosocomialis M2

48.93

95.058

0.465
  pilT Acinetobacter baumannii strain A118

48.93

95.058

0.465
  pilT Neisseria meningitidis 8013

50

93.023

0.465
  pilT Neisseria gonorrhoeae MS11

49.533

93.314

0.462
  pilT Acinetobacter baylyi ADP1

48.615

94.477

0.459
  pilT Legionella pneumophila strain ERS1305867

47.975

93.314

0.448
  pilT Legionella pneumophila strain Lp02

47.975

93.314

0.448
  pilU Pseudomonas stutzeri DSM 10701

42.47

96.512

0.41
  pilU Vibrio cholerae strain A1552

40.673

95.058

0.387
  pilU Acinetobacter baylyi ADP1

40.439

92.733

0.375

Multiple sequence alignment