Detailed information    

insolico Bioinformatically predicted

Overview

Name   comC   Type   Machinery gene
Locus tag   AFK25_RS10985 Genome accession   NZ_CP012152
Coordinates   2083698..2084447 (-) Length   249 a.a.
NCBI ID   WP_035065523.1    Uniprot ID   A0AAW7TFG3
Organism   Anoxybacillus gonensis strain G2     
Function   processing and translocation of ComGC; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2078698..2089447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AFK25_RS10950 (AFK25_10970) radC 2078808..2079482 (-) 675 WP_006320431.1 RadC family protein -
  AFK25_RS10955 (AFK25_10975) - 2079511..2080080 (-) 570 WP_035065539.1 Maf family protein -
  AFK25_RS10960 (AFK25_10980) - 2080195..2080968 (-) 774 WP_035065536.1 SPOR domain-containing protein -
  AFK25_RS10965 (AFK25_10985) - 2081075..2081515 (-) 441 WP_009361738.1 type II secretion system protein -
  AFK25_RS10970 (AFK25_10990) pilO 2081529..2082155 (-) 627 WP_035065532.1 type 4a pilus biogenesis protein PilO -
  AFK25_RS10975 (AFK25_10995) - 2082152..2082733 (-) 582 WP_035065529.1 PilN domain-containing protein -
  AFK25_RS10980 (AFK25_11000) pilM 2082717..2083685 (-) 969 WP_035065526.1 type IV pilus biogenesis protein PilM -
  AFK25_RS10985 (AFK25_11005) comC 2083698..2084447 (-) 750 WP_035065523.1 prepilin peptidase Machinery gene
  AFK25_RS10990 (AFK25_11010) - 2084504..2085019 (-) 516 WP_035065520.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AFK25_RS10995 (AFK25_11015) - 2085095..2086306 (-) 1212 WP_035065517.1 type II secretion system F family protein -
  AFK25_RS11000 (AFK25_11020) pilT 2086312..2087346 (-) 1035 WP_035065514.1 type IV pilus twitching motility protein PilT Machinery gene
  AFK25_RS11005 (AFK25_11025) - 2087357..2089018 (-) 1662 WP_035065511.1 GspE/PulE family protein -

Sequence

Protein


Download         Length: 249 a.a.        Molecular weight: 27150.94 Da        Isoelectric Point: 9.6704

>NTDB_id=151923 AFK25_RS10985 WP_035065523.1 2083698..2084447(-) (comC) [Anoxybacillus gonensis strain G2]
MLLSISFLLGLFLGSFYNVVGLRVPKGESIVAPRSHCPHCKRTLTALELIPVISYVLQKGKCRACSARISFVYPLIELST
AILFTLAPLFVGWSAEVAVSWTLISLFVIIFVSDVHYMIIPNRVLLFFTPLLLVERLWVAQLTPWWDSLLGSVVGFMILF
VIALVSKGGMGGGDIKLFSLIGLALGAKLTVLAFFLSTLVGTIFGLIGMAIGRVKRGEPMPFGPAIVVGTLIAYFFGDTI
IDAYVKVMM

Nucleotide


Download         Length: 750 bp        

>NTDB_id=151923 AFK25_RS10985 WP_035065523.1 2083698..2084447(-) (comC) [Anoxybacillus gonensis strain G2]
ATGTTGCTTTCTATTTCTTTCCTCCTCGGTCTTTTTCTCGGCTCTTTTTATAATGTTGTTGGGTTAAGGGTACCGAAAGG
GGAGTCGATTGTTGCGCCGCGTTCGCATTGTCCGCATTGTAAGCGGACGTTGACGGCGTTAGAGTTAATTCCGGTTATTT
CGTATGTGTTGCAAAAAGGGAAATGTCGCGCGTGTTCGGCGCGCATTTCCTTTGTTTATCCGTTGATTGAGTTGTCCACG
GCTATATTGTTTACGCTCGCCCCGCTTTTTGTCGGTTGGTCAGCGGAAGTAGCCGTTAGTTGGACGCTCATTTCTTTATT
CGTTATTATTTTTGTGTCTGATGTGCATTATATGATTATCCCAAACCGTGTTTTATTATTTTTTACTCCACTTCTTCTTG
TTGAACGTTTATGGGTTGCTCAGCTTACTCCTTGGTGGGATAGTTTACTCGGTAGTGTTGTTGGATTTATGATATTGTTT
GTGATAGCGCTTGTGAGTAAAGGTGGCATGGGTGGTGGAGATATTAAACTATTTTCGTTAATCGGTTTAGCGCTTGGTGC
AAAGCTTACCGTTCTAGCTTTCTTTTTATCGACGCTCGTCGGGACAATATTCGGACTGATTGGTATGGCAATTGGCCGTG
TGAAACGAGGCGAACCGATGCCGTTCGGACCAGCGATCGTCGTTGGGACGTTAATCGCTTATTTTTTCGGAGATACGATC
ATCGATGCGTACGTAAAGGTTATGATGTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(8-87)

Type IV leader peptidase family (Peptidase_A24)

(103-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comC Bacillus subtilis subsp. subtilis str. 168

49.597

99.598

0.494
  pilD Vibrio cholerae strain A1552

32.042

100

0.365

Multiple sequence alignment