Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACJ67_RS05040 Genome accession   NZ_CP012020
Coordinates   1042174..1043217 (+) Length   347 a.a.
NCBI ID   WP_018985802.1    Uniprot ID   -
Organism   Methylophilus sp. TWE2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1037174..1048217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ67_RS05015 (ACJ67_05015) - 1037873..1038445 (+) 573 WP_049638133.1 nucleoside triphosphate pyrophosphatase -
  ACJ67_RS05020 (ACJ67_05020) rng 1038487..1039953 (+) 1467 WP_197080691.1 ribonuclease G -
  ACJ67_RS05025 (ACJ67_05025) - 1039983..1040519 (-) 537 WP_231587258.1 YggT family protein -
  ACJ67_RS05030 (ACJ67_05030) proC 1040531..1041364 (-) 834 WP_049638136.1 pyrroline-5-carboxylate reductase -
  ACJ67_RS05035 (ACJ67_05035) - 1041369..1042085 (-) 717 WP_049638137.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJ67_RS05040 (ACJ67_05040) pilT 1042174..1043217 (+) 1044 WP_018985802.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJ67_RS05045 (ACJ67_05045) pilU 1043222..1044382 (+) 1161 WP_049638138.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJ67_RS05050 (ACJ67_05050) - 1044449..1045741 (-) 1293 WP_049638139.1 dihydroorotase -
  ACJ67_RS05055 (ACJ67_05055) - 1045738..1046694 (-) 957 WP_018985799.1 aspartate carbamoyltransferase catalytic subunit -
  ACJ67_RS05060 (ACJ67_05060) pyrR 1046687..1047244 (-) 558 WP_049638140.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACJ67_RS05065 (ACJ67_05065) ruvX 1047257..1047760 (-) 504 WP_049638141.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38395.01 Da        Isoelectric Point: 6.5055

>NTDB_id=150419 ACJ67_RS05040 WP_018985802.1 1042174..1043217(+) (pilT) [Methylophilus sp. TWE2]
MDISDLLAFSVKNKASDLHLSSGLPPMIRVHGDVRKINLPAMEHAEVHRMLYDIMNDSQRKVYEETLECDFSFEIPDLAR
FRVNAFNQQRGAGAVFRTIPSKVLTLDELNAPKVFKDIADTPRGIVLVTGPTGSGKSTTLAAMIDYINETQMSHVLTVED
PIEFVHTSKKSLINQREVGPHTQSFENALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLCKTKDEQGRVAAHEIMMGTPAIRNLIRENKIAQMYSAIQTGQSMGMQTLDQN
LQDLVKRNVISAAEARTKAANRDAIIG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=150419 ACJ67_RS05040 WP_018985802.1 1042174..1043217(+) (pilT) [Methylophilus sp. TWE2]
GTGGATATTTCAGATTTATTGGCATTTTCAGTTAAAAACAAAGCCTCGGACTTGCATTTGTCCTCCGGTTTGCCGCCGAT
GATTCGCGTACATGGCGATGTGCGTAAAATCAATTTACCTGCCATGGAGCATGCTGAAGTACACCGCATGCTGTATGACA
TCATGAACGACAGCCAGCGCAAGGTGTATGAAGAAACACTGGAATGCGACTTTTCGTTTGAAATTCCTGACCTCGCACGC
TTCCGGGTTAACGCGTTTAACCAGCAACGCGGTGCCGGTGCGGTATTCCGTACCATTCCAAGCAAAGTGCTAACACTGGA
TGAGCTAAATGCCCCCAAGGTGTTCAAGGACATTGCGGATACGCCACGCGGGATTGTACTGGTGACCGGTCCGACTGGTT
CTGGTAAGTCCACCACATTGGCGGCGATGATTGATTACATCAATGAAACGCAGATGTCGCACGTGCTGACTGTGGAAGAC
CCGATTGAATTTGTGCATACCTCGAAAAAGTCATTGATTAACCAGCGCGAAGTCGGTCCGCATACGCAATCGTTTGAAAA
TGCGCTGCGCTCTGCCTTGCGTGAAGACCCGGACGTGATCCTGGTGGGTGAGATGCGTGACCTGGAAACCATCCGCCTGG
CACTGACCGCTGCGGAAACCGGCCACCTGGTGTTTGGTACCTTGCATACCAGCTCCGCCGCCAAAACCATAGACCGGGTG
GTGGACGTGTTCCCGGCTGCTGAAAAAGAAATGGTACGTTCCATGTTATCTGAGTCTTTGCGCGCCGTTATTTCACAAAC
CCTGTGTAAAACCAAAGATGAGCAGGGTCGTGTGGCTGCACATGAAATCATGATGGGTACACCCGCCATCCGTAACCTGA
TTCGTGAAAACAAGATTGCACAGATGTATTCAGCCATCCAGACCGGGCAGAGCATGGGTATGCAAACCCTGGACCAGAAC
CTGCAAGACCTGGTGAAACGAAATGTGATCAGCGCTGCTGAAGCACGCACCAAGGCCGCCAACCGTGACGCTATCATCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.301

99.712

0.761
  pilT Acinetobacter baumannii D1279779

76.301

99.712

0.761
  pilT Acinetobacter baumannii strain A118

76.301

99.712

0.761
  pilT Pseudomonas aeruginosa PAK

76.106

97.695

0.744
  pilT Pseudomonas stutzeri DSM 10701

75.811

97.695

0.741
  pilT Acinetobacter baylyi ADP1

74.631

97.695

0.729
  pilT Neisseria meningitidis 8013

71.884

99.424

0.715
  pilT Neisseria gonorrhoeae MS11

71.594

99.424

0.712
  pilT Legionella pneumophila strain Lp02

71.637

98.559

0.706
  pilT Legionella pneumophila strain ERS1305867

71.637

98.559

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.456

97.406

0.657
  pilT Vibrio cholerae strain A1552

67.456

97.406

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.172

95.389

0.507
  pilU Acinetobacter baylyi ADP1

40.882

97.983

0.401
  pilU Vibrio cholerae strain A1552

41.194

96.542

0.398
  pilU Pseudomonas stutzeri DSM 10701

40

96.542

0.386

Multiple sequence alignment