Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACJ67_RS05045 Genome accession   NZ_CP012020
Coordinates   1043222..1044382 (+) Length   386 a.a.
NCBI ID   WP_049638138.1    Uniprot ID   A0A0K0SUC2
Organism   Methylophilus sp. TWE2     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1038222..1049382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ67_RS05020 (ACJ67_05020) rng 1038487..1039953 (+) 1467 WP_197080691.1 ribonuclease G -
  ACJ67_RS05025 (ACJ67_05025) - 1039983..1040519 (-) 537 WP_231587258.1 YggT family protein -
  ACJ67_RS05030 (ACJ67_05030) proC 1040531..1041364 (-) 834 WP_049638136.1 pyrroline-5-carboxylate reductase -
  ACJ67_RS05035 (ACJ67_05035) - 1041369..1042085 (-) 717 WP_049638137.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJ67_RS05040 (ACJ67_05040) pilT 1042174..1043217 (+) 1044 WP_018985802.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJ67_RS05045 (ACJ67_05045) pilU 1043222..1044382 (+) 1161 WP_049638138.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJ67_RS05050 (ACJ67_05050) - 1044449..1045741 (-) 1293 WP_049638139.1 dihydroorotase -
  ACJ67_RS05055 (ACJ67_05055) - 1045738..1046694 (-) 957 WP_018985799.1 aspartate carbamoyltransferase catalytic subunit -
  ACJ67_RS05060 (ACJ67_05060) pyrR 1046687..1047244 (-) 558 WP_049638140.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACJ67_RS05065 (ACJ67_05065) ruvX 1047257..1047760 (-) 504 WP_049638141.1 Holliday junction resolvase RuvX -
  ACJ67_RS05070 (ACJ67_05070) - 1047753..1048358 (-) 606 WP_049638142.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 386 a.a.        Molecular weight: 43132.61 Da        Isoelectric Point: 6.6612

>NTDB_id=150420 ACJ67_RS05045 WP_049638138.1 1043222..1044382(+) (pilU) [Methylophilus sp. TWE2]
MESTQTVGDHAQTEKFVNDLLKLMITRKASDLFITAGFPPAMKIDGKLTPVSSQMLNTQQTAEIARTVMNDKQAQEFKET
LECNFAISMPSVGRFRVNAFIQRSAVGLVFRVINTNIPKFDDLKLPPILKDVVMAKRGLIIFVGGTGSGKSTSMAAMLGH
RNESSYGHIITIEDPVEFIHGHKNCIVTQREVGVDTESWHAALKNTLRQAPDVIMIGEIRDRETMDYAIAFSETGHLCLA
TLHANSTNQALDRIINFFPEERRQQLLMDLSLNLRCIASQRLIPKLDSSGRVAAVEVLLHSPLISDLIYKGDVHAIKEIM
AKSRELGMQTFDQALFDLFESGQISYDDALRNADSVNDLRLKFKLNSKLMNPENQERGQISNLDIL

Nucleotide


Download         Length: 1161 bp        

>NTDB_id=150420 ACJ67_RS05045 WP_049638138.1 1043222..1044382(+) (pilU) [Methylophilus sp. TWE2]
ATGGAGAGCACGCAAACCGTGGGCGATCACGCCCAGACGGAAAAATTCGTCAATGACTTGCTCAAGCTCATGATCACGCG
CAAGGCCTCTGACTTGTTCATCACGGCGGGTTTCCCACCAGCCATGAAGATAGACGGCAAGCTGACCCCCGTCTCATCGC
AAATGCTCAATACACAGCAAACCGCAGAAATTGCGCGTACTGTCATGAACGACAAGCAGGCGCAGGAGTTCAAGGAGACA
CTGGAGTGTAACTTTGCCATCAGCATGCCTTCTGTAGGCCGCTTCCGCGTTAATGCTTTTATCCAGCGCAGTGCAGTCGG
CCTGGTGTTCCGCGTCATTAATACCAACATCCCCAAATTTGACGACCTCAAGCTGCCGCCCATCCTCAAGGATGTGGTCA
TGGCCAAGCGTGGCCTGATCATTTTTGTGGGTGGCACCGGCTCCGGTAAGTCGACCTCCATGGCTGCGATGCTAGGTCAC
CGCAATGAAAGCAGTTACGGCCATATTATTACGATTGAAGACCCGGTCGAATTTATTCATGGCCACAAAAATTGCATTGT
GACGCAGCGTGAAGTCGGAGTGGATACCGAGAGCTGGCATGCTGCGCTCAAGAACACTTTGCGTCAGGCGCCGGATGTGA
TCATGATCGGCGAGATCCGCGACCGTGAAACCATGGATTACGCGATTGCTTTCTCTGAGACTGGGCATTTGTGCCTGGCA
ACCCTGCATGCCAACAGCACCAACCAGGCACTGGACCGGATTATCAACTTTTTCCCCGAAGAGCGCCGCCAGCAGTTACT
CATGGATTTGTCGCTTAACCTGCGTTGTATCGCTTCACAACGCCTTATTCCCAAACTGGATAGCAGTGGCCGTGTGGCGG
CAGTAGAAGTGTTACTGCATTCGCCACTGATCTCAGACCTGATTTACAAGGGGGATGTGCATGCCATCAAGGAAATCATG
GCCAAATCCCGTGAGCTGGGCATGCAAACCTTTGATCAGGCCTTGTTTGATTTATTTGAGTCTGGCCAGATCAGTTATGA
CGATGCTTTGCGTAACGCAGACTCGGTCAACGACTTGCGGCTTAAATTCAAGCTGAACAGCAAGCTGATGAACCCTGAAA
ACCAGGAACGCGGGCAGATTTCTAACCTGGATATCCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K0SUC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

61.326

93.782

0.575
  pilU Pseudomonas stutzeri DSM 10701

62.178

90.415

0.562
  pilU Vibrio cholerae strain A1552

55.46

90.155

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.136

87.306

0.368

Multiple sequence alignment